Region-specific RNA m6A methylation represents a new layer of control in the gene regulatory network in the mouse brain

Autor: Mengqi Chang, Hongyi Lv, Weilong Zhang, Chunhui Ma, Xue He, Shunli Zhao, Zhi-Wei Zhang, Yi-Xin Zeng, Shuhui Song, Yamei Niu, Wei-Min Tong

Publikováno v: Open Biology, Vol 7, Iss 9 (2017)

N6-methyladenosine (m6A) is the most abundant epitranscriptomic mark found on mRNA and has important roles in various physiological processes. Despite the relatively high m6A levels in the brain, its potential functions in the brain remain largely un

Externí odkaz: https://doaj.org/article/29326f8e71fb4c81af111e0c3e0019d0

Isolation of myeloid cells from mouse brain tumors for single-cell RNA-seq analysis

Autor: Fernanda Abani Mafra, Malay Haldar, Mai T Dang

Publikováno v: STAR Protocols
STAR Protocols, Vol 2, Iss 4, Pp 100957-(2021)

Summary Current single-cell RNA sequencing (scRNA-seq) protocols are limited by the number of cells that can be simultaneously sequenced, restricting the ability to resolve heterogeneity of rare cell types. We describe here a protocol for rapid isola

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43da2ebe7aaf47298c0e95e3f52473bd
http://europepmc.org/articles/PMC8605103

Generation of excitatory and inhibitory neurons from common progenitors via Notch signaling in the cerebellum

Autor: Andi H. Hansen, Natalia Mora, Natalia Danda, Justine Guegan, Luca Tiberi, Simon Hippenmeyer, Mathilde Bertrand, Marica Anderle, Tingting Zhang, Carmen Streicher, Tengyuan Liu, Ximena Contreras, Bassem A. Hassan

Publikováno v: bioRxiv
Cell Reports
Cell Reports, Elsevier Inc, 2021, 35 (10), pp.109208. ⟨10.1016/j.celrep.2021.109208⟩
Cell Reports, 2021, 35 (10), pp.109208. ⟨10.1016/j.celrep.2021.109208⟩

SUMMARYBrain neurons arise from relatively few progenitors capable of giving rise to an enormous diversity of neuronal types. Nonetheless, a cardinal feature of mammalian brain neurogenesis in both the cortex and the cerebellum is that excitatory neu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e97ae2bf51261f4e2ca98fa37657f906
https://lirias.kuleuven.be/handle/20.500.12942/688187

Notch1 switches progenitor competence in inducing medulloblastoma

Autor: Matteo Gianesello, Tingting Zhang, Giuseppe Aiello, Francesca Gianno, Luca Tiberi, Francesco Antonica, Bassem A. Hassan, Felice Giangaspero, Konstantin Okonechnikov, Claudio Ballabio, Chiara Lago, Stefan M. Pfister, Marica Anderle

Publikováno v: Science Advances
Science Advances, American Association for the Advancement of Science (AAAS), 2021, 7 (26), pp.eabd2781. ⟨10.1126/sciadv.abd2781⟩
Science Advances, 2021, 7 (26), pp.eabd2781. ⟨10.1126/sciadv.abd2781⟩

Notch1 pathway activation is required for group 3 medulloblastoma initiation.
The identity of the cell of origin is a key determinant of cancer subtype, progression, and prognosis. Group 3 medulloblastoma (MB) is a malignant childhood brain canc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d67f50fb612bcf0113692e78522c247f
https://hal.sorbonne-universite.fr/hal-03272112/document

Advanced Light-Sheet Microscopy to Explore Brain Structure on an Organ-Wide Scale

Autor: Ludovico Silvestri, Francesco S. Pavone

Publikováno v: Handbook of Neurophotonics ISBN: 9780429194702

Light-sheet microscopy has emerged as the method of choice to perform brain studies the at whole-organ scale. The brain is an extremely complex organ, whose structure and function spans many orders of magnitude. Single-cell mapping in entire cleared

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ca518361aab3ce0403abe5bdc885b240
https://doi.org/10.1201/9780429194702-11