An antibody raised against a pathogenic serpin variant induces mutant-like behaviour in the wild-type protein

Autor: Irving, J. A., MIRANDA BANOS, MARIA ELENA, Haq, I., Perez, J., Kotov, V. R., Faull, S. V., Motamedi Shad, N., Lomas, D. A.

Publikováno v: Biochemical Journal

A monoclonal antibody (mAb) that binds to a transient intermediate may act as a catalyst for the corresponding reaction; here we show this principle can extend on a macro molecular scale to the induction of mutant-like oligomerization in a wild-type

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::8557a4112b4d3b5087bf6b308b2a138f
http://europepmc.org/articles/PMC4422257

An antibody that prevents serpin polymerisation acts by inducing a novel allosteric behaviour

Autor: Motamedi-Shad, N, Jagger, Am, Liedtke, M, Faull, Sv, Nanda, As, Salvadori, E, Wort, Jl, Kay, Cwm, Heyer-Chauhan, N, Miranda, E, Perez, J, Ordonez, A, Haq, I, Irving, Ja, Lomas, Da

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od_______970::10b117894b5a7870b211fa20548af895
http://hdl.handle.net/2318/1690235

Kinetic analysis of amyloid formation in the presence of heparan sulfate. Faster unfolding and change of pathway

Autor: MOTAMEDI SHAD, N., Monsellier, E., Torrassa, S., Relini, Annalisa, Chiti, F.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od______3710::5ada2f7a6ac2d71a95cfdd49ad8bab90
http://hdl.handle.net/11567/219857

An antibody that prevents serpin polymerisation acts by inducing a novel allosteric behaviour.

Autor: Motamedi-Shad N; Centre for Respiratory Biology, UCL Respiratory, University College London, London WC1E 6JF, U.K. Institute of Structural and Molecular Biology/Birkbeck, University of London, London WC1E 7HX, U.K., Jagger AM; Centre for Respiratory Biology, UCL Respiratory, University College London, London WC1E 6JF, U.K. Institute of Structural and Molecular Biology/Birkbeck, University of London, London WC1E 7HX, U.K., Liedtke M; Centre for Respiratory Biology, UCL Respiratory, University College London, London WC1E 6JF, U.K., Faull SV; Centre for Respiratory Biology, UCL Respiratory, University College London, London WC1E 6JF, U.K. Institute of Structural and Molecular Biology/Birkbeck, University of London, London WC1E 7HX, U.K. Department of Medicine, University of Cambridge, Cambridge Institute for Medical Research, Wellcome Trust/Medical Research Council Building, Hills Road, Cambridge CB2 0XY, U.K., Nanda AS; Institute of Structural and Molecular Biology/Birkbeck, University of London, London WC1E 7HX, U.K., Salvadori E; Institute of Structural and Molecular Biology/Birkbeck, University of London, London WC1E 7HX, U.K. London Centre for Nanotechnology, 17-19 Gordon Street, London WC1H 0AH, U.K. School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, U.K., Wort JL; Institute of Structural and Molecular Biology/Birkbeck, University of London, London WC1E 7HX, U.K., Kay CW; Institute of Structural and Molecular Biology/Birkbeck, University of London, London WC1E 7HX, U.K. London Centre for Nanotechnology, 17-19 Gordon Street, London WC1H 0AH, U.K., Heyer-Chauhan N; Centre for Respiratory Biology, UCL Respiratory, University College London, London WC1E 6JF, U.K. Institute of Structural and Molecular Biology/Birkbeck, University of London, London WC1E 7HX, U.K., Miranda E; Department of Biology and Biotechnologies 'Charles Darwin', Sapienza University of Rome, Rome 00185, Italy., Perez J; Departamento de Biologia Celular, Genetica y Fisiologia, Facultad de Ciencias, Campus Teatinos, Universidad de Malaga, Malaga 29071, Spain., Ordóñez A; Department of Medicine, University of Cambridge, Cambridge Institute for Medical Research, Wellcome Trust/Medical Research Council Building, Hills Road, Cambridge CB2 0XY, U.K., Haq I; Centre for Respiratory Biology, UCL Respiratory, University College London, London WC1E 6JF, U.K. Institute of Structural and Molecular Biology/Birkbeck, University of London, London WC1E 7HX, U.K., Irving JA; Centre for Respiratory Biology, UCL Respiratory, University College London, London WC1E 6JF, U.K. Institute of Structural and Molecular Biology/Birkbeck, University of London, London WC1E 7HX, U.K., Lomas DA; Centre for Respiratory Biology, UCL Respiratory, University College London, London WC1E 6JF, U.K. Institute of Structural and Molecular Biology/Birkbeck, University of London, London WC1E 7HX, U.K.

Publikováno v: The Biochemical journal [Biochem J] 2016 Oct 01; Vol. 473 (19), pp. 3269-90. Date of Electronic Publication: 2016 Jul 12.

A single-chain variable fragment intrabody prevents intracellular polymerization of Z α1-antitrypsin while allowing its antiproteinase activity.

Autor: Ordóñez A; Department of Medicine, University of Cambridge, Cambridge Institute for Medical Research, Cambridge, United Kingdom; Department of Cell Biology, Genetics and Physiology, University of Malaga, Malaga, Spain; Wolfson Institute for Biomedical Research, University College London, London, United Kingdom; and Department of Biology and Biotechnologies, 'Charles Darwin,' and Pasteur Institute, Cenci Bolognetti Foundation, Sapienza University, Rome, Italy., Pérez J; Department of Medicine, University of Cambridge, Cambridge Institute for Medical Research, Cambridge, United Kingdom; Department of Cell Biology, Genetics and Physiology, University of Malaga, Malaga, Spain; Wolfson Institute for Biomedical Research, University College London, London, United Kingdom; and Department of Biology and Biotechnologies, 'Charles Darwin,' and Pasteur Institute, Cenci Bolognetti Foundation, Sapienza University, Rome, Italy., Tan L; Department of Medicine, University of Cambridge, Cambridge Institute for Medical Research, Cambridge, United Kingdom; Department of Cell Biology, Genetics and Physiology, University of Malaga, Malaga, Spain; Wolfson Institute for Biomedical Research, University College London, London, United Kingdom; and Department of Biology and Biotechnologies, 'Charles Darwin,' and Pasteur Institute, Cenci Bolognetti Foundation, Sapienza University, Rome, Italy., Dickens JA; Department of Medicine, University of Cambridge, Cambridge Institute for Medical Research, Cambridge, United Kingdom; Department of Cell Biology, Genetics and Physiology, University of Malaga, Malaga, Spain; Wolfson Institute for Biomedical Research, University College London, London, United Kingdom; and Department of Biology and Biotechnologies, 'Charles Darwin,' and Pasteur Institute, Cenci Bolognetti Foundation, Sapienza University, Rome, Italy., Motamedi-Shad N; Department of Medicine, University of Cambridge, Cambridge Institute for Medical Research, Cambridge, United Kingdom; Department of Cell Biology, Genetics and Physiology, University of Malaga, Malaga, Spain; Wolfson Institute for Biomedical Research, University College London, London, United Kingdom; and Department of Biology and Biotechnologies, 'Charles Darwin,' and Pasteur Institute, Cenci Bolognetti Foundation, Sapienza University, Rome, Italy., Irving JA; Department of Medicine, University of Cambridge, Cambridge Institute for Medical Research, Cambridge, United Kingdom; Department of Cell Biology, Genetics and Physiology, University of Malaga, Malaga, Spain; Wolfson Institute for Biomedical Research, University College London, London, United Kingdom; and Department of Biology and Biotechnologies, 'Charles Darwin,' and Pasteur Institute, Cenci Bolognetti Foundation, Sapienza University, Rome, Italy., Haq I; Department of Medicine, University of Cambridge, Cambridge Institute for Medical Research, Cambridge, United Kingdom; Department of Cell Biology, Genetics and Physiology, University of Malaga, Malaga, Spain; Wolfson Institute for Biomedical Research, University College London, London, United Kingdom; and Department of Biology and Biotechnologies, 'Charles Darwin,' and Pasteur Institute, Cenci Bolognetti Foundation, Sapienza University, Rome, Italy., Ekeowa U; Department of Medicine, University of Cambridge, Cambridge Institute for Medical Research, Cambridge, United Kingdom; Department of Cell Biology, Genetics and Physiology, University of Malaga, Malaga, Spain; Wolfson Institute for Biomedical Research, University College London, London, United Kingdom; and Department of Biology and Biotechnologies, 'Charles Darwin,' and Pasteur Institute, Cenci Bolognetti Foundation, Sapienza University, Rome, Italy., Marciniak SJ; Department of Medicine, University of Cambridge, Cambridge Institute for Medical Research, Cambridge, United Kingdom; Department of Cell Biology, Genetics and Physiology, University of Malaga, Malaga, Spain; Wolfson Institute for Biomedical Research, University College London, London, United Kingdom; and Department of Biology and Biotechnologies, 'Charles Darwin,' and Pasteur Institute, Cenci Bolognetti Foundation, Sapienza University, Rome, Italy., Miranda E; Department of Medicine, University of Cambridge, Cambridge Institute for Medical Research, Cambridge, United Kingdom; Department of Cell Biology, Genetics and Physiology, University of Malaga, Malaga, Spain; Wolfson Institute for Biomedical Research, University College London, London, United Kingdom; and Department of Biology and Biotechnologies, 'Charles Darwin,' and Pasteur Institute, Cenci Bolognetti Foundation, Sapienza University, Rome, Italy mariaelena.mirandabanos@uniroma1.it d.lomas@ucl.ac.uk., Lomas DA; Department of Medicine, University of Cambridge, Cambridge Institute for Medical Research, Cambridge, United Kingdom; Department of Cell Biology, Genetics and Physiology, University of Malaga, Malaga, Spain; Wolfson Institute for Biomedical Research, University College London, London, United Kingdom; and Department of Biology and Biotechnologies, 'Charles Darwin,' and Pasteur Institute, Cenci Bolognetti Foundation, Sapienza University, Rome, Italy mariaelena.mirandabanos@uniroma1.it d.lomas@ucl.ac.uk.

Publikováno v: FASEB journal : official publication of the Federation of American Societies for Experimental Biology [FASEB J] 2015 Jun; Vol. 29 (6), pp. 2667-78. Date of Electronic Publication: 2015 Mar 10.