DNA-binding mechanism and evolution of replication protein A

Autor: Clément Madru, Markel Martínez-Carranza, Sébastien Laurent, Alessandra C. Alberti, Maelenn Chevreuil, Bertrand Raynal, Ahmed Haouz, Rémy A. Le Meur, Marc Delarue, Ghislaine Henneke, Didier Flament, Mart Krupovic, Pierre Legrand, Ludovic Sauguet

Publikováno v: Nature Communications
Nature Communications, 2023, 14 (1), pp.2326. ⟨10.1038/s41467-023-38048-w⟩
Nature Communications (2041-1723) (Springer Science and Business Media LLC), 2023-04, Vol. 14, N. 1, P. 2326 (14p.)

Replication Protein A (RPA) is a heterotrimeric single stranded DNA-binding protein with essential roles in DNA replication, recombination and repair. Little is known about the structure of RPA in Archaea, the third domain of life. By using an integr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd2b10211d6b8211b7c6c5fbe1236951
https://doi.org/10.1038/s41467-023-38048-w

The coordinated replication of Vibrio cholerae’s two chromosomes required the acquisition of a unique domain by the RctB initiator

Autor: Fournes, Florian, Niault, Theophile, Czarnecki, Jakub, Tissier-Visconti, Alvise, Mazel, Didier, Val, Marie-Eve

Publikováno v: Nucleic Acids Research
Nucleic Acids Research, 2021, 49 (19), pp.11119-11133. ⟨10.1093/nar/gkab903⟩
Nucleic Acids Research, Oxford University Press, 2021, 49 (19), pp.11119-11133. ⟨10.1093/nar/gkab903⟩

International audience; Vibrio cholerae, the pathogenic bacterium that causes cholera, has two chromosomes (Chr1, Chr2) that replicate in a well-orchestrated sequence. Chr2 initiation is triggered only after the replication of the crtS site on Chr1.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::78989246a70ed6b9797197547822388d
http://europepmc.org/articles/PMC8565311

Transcription-dependent regulation of replication dynamics modulates genome stability

Autor: Marion Blin, Caroline Brossas, Gaël A. Millot, Mélanie Schmidt, Viola Nähse, Marie-Noëlle Prioleau, Michelle Debatisse, Benoît Le Tallec

Publikováno v: Nature Structural and Molecular Biology
Nature Structural and Molecular Biology, Nature Publishing Group, 2019, 26 (1), pp.58-66. ⟨10.1038/s41594-018-0170-1⟩
Nature Structural and Molecular Biology, 2019, 26 (1), pp.58-66. ⟨10.1038/s41594-018-0170-1⟩

International audience; Common fragile sites (CFSs) are loci that are hypersensitive to replication stress and hotspots for chromosomal rearrangements in cancers. CFSs replicate late in S phase, are cell-type specific and nest in large genes. The rel

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43eeaf52d0908fdafd2f3542152ba98e
https://doi.org/10.1038/s41594-018-0170-1

Noncanonical DNA polymerization by aminoadenine-based siphoviruses

Autor: Faten Jaziri, Philippe Marliere, Dominique Louis, Sylvie Pochet, Graham F. Hatfull, Jef Rozenski, Pierre-Yves Bourguignon, Piet Herdewijn, Deborah Jacobs-Sera, Pierre-Alexandre Kaminski, Valérie Pezo

Publikováno v: Science
Science, American Association for the Advancement of Science, 2021, 372 (6541), pp.520-524. ⟨10.1126/science.abe6542⟩
Science, 2021, 372 (6541), pp.520-524. ⟨10.1126/science.abe6542⟩

Biosynthesis and replication, from A to Z Four nucleobases. adenine (A), cytosine (C), guanine (G), and thymine (T), are usually thought to be invariable in DNA. In bacterial viruses, however, each of the DNA bases have variations that help them to e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4fbb84b511d1fb1228b14533a9f925c
https://hal-pasteur.archives-ouvertes.fr/pasteur-03228903

Novel ribonucleotide discrimination in the RNA polymerase-like two-barrel catalytic core of Family D DNA polymerases

Autor: Thomas C. Evans, Andrew F. Gardner, Ece Alpaslan, Kelly M Zatopek, Ludovic Sauguet

Publikováno v: Nucleic Acids Research
Nucleic Acids Research, Oxford University Press, 2020, 48 (21), pp.12204-12218. ⟨10.1093/nar/gkaa986⟩
Nucleic Acids Research, 2020, 48 (21), pp.12204-12218. ⟨10.1093/nar/gkaa986⟩

Family D DNA polymerase (PolD) is the essential replicative DNA polymerase for duplication of most archaeal genomes. PolD contains a unique two-barrel catalytic core absent from all other DNA polymerase families but found in RNA polymerases (RNAPs).

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85fbf1b87ad50a09fe8aa5c33da49784
https://hal-pasteur.archives-ouvertes.fr/pasteur-03086156/document