Zobrazeno 1 - 10 of 402 pro vyhledávání: '"MESH: Recombinant Fusion Proteins"'
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Developing a tetO/TetR system in Neurospora crassa
Autor: Eugene Gladyshev, Tinh-Suong Nguyen
Publikováno v: Fungal Genetics and Biology
Fungal Genetics and Biology, 2020, 136, pp.103316. ⟨10.1016/j.fgb.2019.103316⟩
Fungal Genetics and Biology, Elsevier, 2020, 136, pp.103316. ⟨10.1016/j.fgb.2019.103316⟩
International audience; The development of a tetO/TetR system in the fungus Neurospora crassa is described. The system includes (i) a synthetic gene encoding a TetR variant fused to GFP, and (ii) a standard tetO array integrated homologously, as a pr
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e81a2b1fb27dab10d68f603a8b275d78
https://hal-pasteur.archives-ouvertes.fr/pasteur-03261456/document
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3
Design of multi-epitope peptides containing HLA class-I and class-II-restricted epitopes derived from immunogenic Leishmania proteins, and evaluation of CD4+ and CD8+ T cell responses induced in cured cutaneous leishmaniasis subjects
Autor: Yasmine Messaoudi, Elodie Petitdidier, A. Kidar, Karim Aoun, Jean-Loup Lemesre, Julie Pagniez, Sarra Hamrouni, Rym Chamakh-Ayari, Rachel Bras-Gonçalves, Amel Meddeb-Garnaoui
Publikováno v: PLoS Neglected Tropical Diseases
PLoS Neglected Tropical Diseases, 2020, 14 (3), pp.e0008093. ⟨10.1371/journal.pntd.0008093⟩
PLoS Neglected Tropical Diseases, Vol 14, Iss 3, p e0008093 (2020)
Human leishmaniasis is a public health problem worldwide for which the development of a vaccine remains a challenge. T cell-mediated immune responses are crucial for protection. Peptide vaccines based on the identification of immunodominant T cell ep
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c589b954ab74a2c665af6d9e7878fc8
https://pubmed.ncbi.nlm.nih.gov/32176691
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4
Analysis of Membrane Protein Interactions with a Bacterial Adenylate Cyclase-Based Two-Hybrid (BACTH) Technique
Autor: Daniel Ladant, Marilyne Davi, Gouzel Karimova, Scot P. Ouellette
Publikováno v: Current protocols in molecular biology
Current protocols in molecular biology, Wiley, 2017, 118, pp.20.12.1-20.12.24. ⟨10.1002/cpmb.36⟩
Current Protocols in Molecular Biology
Current Protocols in Molecular Biology, 2017, 118, pp.20.12.1-20.12.24. ⟨10.1002/cpmb.36⟩
The bacterial two-hybrid (BACTH, for "Bacterial Adenylate Cyclase-based Two-Hybrid") technique is a simple and fast genetic approach to analyze protein-protein interactions in vivo. In this system, the proteins of interest are genetically fused to tw
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27c585240cadd70c79abdbcc7c66e044
https://hal-pasteur.archives-ouvertes.fr/pasteur-02879386
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5
Flagellar incorporation of proteins follows at least two different routes in trypanosomes
Autor: Vincensini, Laetitia, Blisnick, Thierry, Bertiaux, Eloise, Hutchinson, Sebastian, Georgikou, Christina, Ooi, Cher-Pheng, Bastin, Philippe
Publikováno v: Biology of the Cell
Biology of the Cell, Wiley, 2018, 110 (2), pp.33-47. ⟨10.1111/boc.201700052⟩
Biology of the Cell, 2018, 110 (2), pp.33-47. ⟨10.1111/boc.201700052⟩
International audience; Background information: Eukaryotic cilia and flagella are sophisticated organelles composed of several hundreds of proteins that need to be incorporated at the right time and the right place during assembly.Results: Two method
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::958b10bb071ba0217846d0c60233a4da
https://hal-pasteur.archives-ouvertes.fr/pasteur-03112358
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6
A factor VIII-nanobody fusion protein forming an ultrastable complex with VWF: effect on clearance and antibody formation
Autor: Vincent Muczynski, Valérie Proulle, Peter J. Lenting, François Moreau, Paulette Legendre, Charlotte Kawecki, Gabriel Aymé, Caterina Casari, Olivier D. Christophe, Cécile V. Denis
Publikováno v: Blood
Blood, American Society of Hematology, 2018, 132 (11), pp.1193-1197. ⟨10.1182/blood-2018-01-829523⟩
International audience; Von Willebrand factor (VWF) modulates factor VIII (FVIII) clearance and the anti-FVIII immune response. Despite the high affinity that defines the FVIII/VWF interaction, association/dissociation kinetics dictates 2% to 5% FVII
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3a2041fb5c4d594c4eedd5d312757f2
https://pubmed.ncbi.nlm.nih.gov/30064978
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7
Visualization of single endogenous polysomes reveals the dynamics of translation in live human cells
Autor: Aubin Samacoits, Adham Safieddine, Racha Chouaib, Marion Peter, Florian Mueller, Eugenia Basyuk, Xavier Pichon, Amandine Bastide, Edouard Bertrand
Publikováno v: Journal of Cell Biology
Journal of Cell Biology, Rockefeller University Press, 2016, 214 (6), pp.769-781. ⟨10.1083/jcb.201605024⟩
Journal of Cell Biology, 2016, 214 (6), pp.769-781. ⟨10.1083/jcb.201605024⟩
Journal of Cell Biology, Rockefeller University Press, 2016, 214 (6), pp.769-781. 〈10.1083/jcb.201605024〉
The Journal of Cell Biology
International audience; Translation is an essential step in gene expression. In this study, we used an improved SunTag system to label nascent proteins and image translation of single messenger ribonucleoproteins (mRNPs) in human cells. Using a dedic
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b2011333d9a2df815ffa8e39a6644f9
https://hal-pasteur.archives-ouvertes.fr/pasteur-01622667
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8
Investigation of rabies virus glycoprotein carboxyl terminus as an in vitro predictive tool of neurovirulence. A 3R approach
Autor: Monique Lafon, Zakir Khan, Claude T. Sabeta, Christophe Prehaud, Wonhyo Seo
Publikováno v: Microbes and Infection
Microbes and Infection, Elsevier, 2017, 19 (9-10), pp.476-484. ⟨10.1016/j.micinf.2017.05.006⟩
Microbes and Infection, 2017, 19 (9-10), pp.476-484. ⟨10.1016/j.micinf.2017.05.006⟩
International audience; In the field of live viral vaccines production, there is an unmet need for in vitro tests complying a 3R approach (Refine, Replace and Reduce the use of animal experimentation) to replace the post-licensing safety tests curren
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11df13638a67bf3895f57baa0f3a6e9c
https://hal-pasteur.archives-ouvertes.fr/pasteur-01783671
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9
Defining Membrane Protein Topology Using pho-lac Reporter Fusions
Autor: Gouzel Karimova, Daniel Ladant
Publikováno v: Bacterial Protein Secretion Systems
Laure Journet; Eric Cascales. Bacterial Protein Secretion Systems, 1615, Humana Press; Springer Science, pp.129-142, 2017, Methods in Molecular Biology, 978-1-4939-7033-9. ⟨10.1007/978-1-4939-7033-9_10⟩
Methods in Molecular Biology ISBN: 9781493970315
International audience; Experimental determination of membrane protein topology can be achieved using various techniques. Here we present the pho-lac dual reporter system, a simple, convenient, and reliable tool to analyze the topology of membrane pr
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8744dea405523c14c0fe1525d9edd55
https://hal-pasteur.archives-ouvertes.fr/pasteur-02878965
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10
Flagellin-Mediated Protection against Intestinal Yersinia pseudotuberculosis Infection Does Not Require Interleukin-22
Autor: Mohamed Lamkanfi, Michel Simonet, Nicolas Jonckheere, Rémi Porte, Delphine Cayet, Benoît Foligné, Laure Dumoutier, Jean-Claude Sirard, Isabelle Van Seuningen, Jean-Christophe Renauld, José A. Chabalgoity, Julien Tabareau, Pierre Gosset, Natalia Muñoz-Wolf, Christophe Carnoy, Laurye Van Maele
Publikováno v: Infection and Immunity
Infection and Immunity, American Society for Microbiology, 2017, 85 (2), pp.e00806-16. ⟨10.1128/IAI.00806-16⟩
Infection and Immunity, Vol. 85, no. 2, p. IAI.00806-16 [1-12] (2017)
Infection and Immunity, 2017, 85 (2), pp.e00806-16. ⟨10.1128/IAI.00806-16⟩
Signaling through Toll-like receptors (TLRs), the main receptors in innate immunity, is essential for the defense of mucosal surfaces. It was previously shown that systemic TLR5 stimulation by bacterial flagellin induces an immediate, transient inter
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1be2a1fcd9a01996b539cd115a9c8c0a
https://europepmc.org/articles/PMC5278166/
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