Single molecule imaging simulations with advanced fluorophore photophysics

Autor: Dominique Bourgeois

Publikováno v: Communications Biology
Communications Biology, 2023, 6 (1), pp.53. ⟨10.1038/s42003-023-04432-x⟩

Advanced fluorescence imaging techniques such as single-molecule localization microscopy (SMLM) fundamentally rely on the photophysical behavior of the employed fluorophores. This behavior is generally complex and impacts data quality in a subtle man

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64a4dc86362688a00bba117db6968656
https://doi.org/10.1038/s42003-023-04432-x

NSs amyloid formation is associated with the virulence of Rift Valley fever virus in mice

Autor: Susann Kummer, Hans-Georg Kräusslich, Psylvia Léger, Steeve Boulant, Jana Koch, Pierre-Yves Lozach, Megan L. Stanifer, Qilin Xin, Michèle Bouloy, Robin Burk, Carmen Nussbaum-Krammer, Carole Tamietti, Marie Flamand, Karsten Richter, Eliana Nachman, Xavier Montagutelli

Publikováno v: Nature Communications, Vol 11, Iss 1, Pp 1-19 (2020)
Nature Communications
Nature Communications, 2020, 11 (1), pp.3281. ⟨10.1038/s41467-020-17101-y⟩
Nature Communications, Nature Publishing Group, 2020, 11 (1), pp.3281. ⟨10.1038/s41467-020-17101-y⟩

Amyloid fibrils result from the aggregation of host cell-encoded proteins, many giving rise to specific human illnesses such as Alzheimer’s disease. Here we show that the major virulence factor of Rift Valley fever virus, the protein NSs, forms fil

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dcefe0a320669003cafef628cf559b3d
https://doaj.org/article/74f1f51789f34696aeebabd489877a8c

mEos4b photoconversion efficiency depends on laser illumination conditions used in PALM

Autor: Jip Wulffele, Daniel Thédié, Oleksandr Glushonkov, Dominique Bourgeois

Publikováno v: Journal of Physical Chemistry Letters
Journal of Physical Chemistry Letters, 2022, 13 (22), pp.5075-5080. ⟨10.1021/acs.jpclett.2c00933⟩

Green-to-red photoconvertible fluorescent proteins (PCFPs) are widely employed as markers in photoactivated localization microscopy (PALM). However, their highly complex photophysical behavior complicates their usage. The fact that only a limited fra

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db215f72e17af5ef63f68221871d52e9
https://doi.org/10.1101/2022.03.31.486573

Easing batch image processing from OMERO: a new toolbox for ImageJ

Autor: Pierre Pouchin, Rayan Zoghlami, Rémi Valarcher, Maxence Delannoy, Manon Carvalho, Clémence Belle, Marc Mongy, Sophie Desset, Frédéric Brau

Publikováno v: F1000Research
F1000Research, 2022, 11, pp.392. ⟨10.12688/f1000research.110385.1⟩

International audience; The Open Microscopy Environment Remote Objects (OMERO) is an open source image manager used by many biologists to store, organize, view and share microscopy images, while the open source software ImageJ/Fiji is a very popular

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d33e23a4a5adfd868e518e94790003d
https://hal.science/hal-03716131

Targeting Tn-Antigen-Positive Human Tumors with a Recombinant Human Macrophage Galactose C-Type Lectin

Autor: François Bulteau, Michel Thépaut, Maxime Henry, Amandine Hurbin, Laetitia Vanwonterghem, Corinne Vivès, Aline Le Roy, Christine Ebel, Olivier Renaudet, Franck Fieschi, Jean-Luc Coll

Publikováno v: Molecular Pharmaceutics
Molecular Pharmaceutics, 2022, 19 (1), pp.235-245. ⟨10.1021/acs.molpharmaceut.1c00744⟩

International audience; Alterations in glycosylation cause the emergence of tumor-associated carbohydrate antigens (TACAs) during tumorigenesis. Truncation of O-glycans reveals the Thomsen nouveau (Tn) antigen, an N-acetylgalactosamine (GalNAc) frequ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4d8115bd4c42ccb6ab3a6a3fa5e5ab90
https://pubmed.ncbi.nlm.nih.gov/34927439