Structure-function-dynamics relationships in the peculiar Planktothrix PCC7805 OCP1: impact of his-tagging and carotenoid type

Autor: Adjélé Wilson, Elena A. Andreeva, Stanislaw J. Nizinski, Léa Talbot, Elisabeth Hartmann, Ilme Schlichting, Gotard Burdzinski, Michel Sliwa, Diana Kirilovsky, Jacques-Philippe Colletier

Publikováno v: Biochimica et Biophysica Acta, Bioenergetics
Biochimica biophysica acta (BBA)-Bioenergetics
Biochimica biophysica acta (BBA)-Bioenergetics, Elsevier, 2022, 1863 (7), pp.148584. ⟨10.1016/j.bbabio.2022.148584⟩
Biochimica biophysica acta (BBA)-Bioenergetics, 2022, 1863 (7), pp.148584. ⟨10.1016/j.bbabio.2022.148584⟩

The orange carotenoid protein (OCP) is a photoactive protein involved in cyanobacterial photoprotection. Here, we report on the functional, spectral and structural characteristics of the peculiar Planktothrix PCC7805 OCP (Plankto-OCP). We show that t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::45027c70d8e60469ff88d974ac463d0a
https://hdl.handle.net/21.11116/0000-000B-3427-F21.11116/0000-0009-BF2A-221.11116/0000-0009-BF2C-0

Stealth fluorescence labeling for live microscopy imaging of mRNA delivery

Autor: Elin K. Esbjörner, Jesper R. Nilsson, Audrey Gallud, Marcel Hollenstein, Cécile Gasse, Emanuele Celauro, Anders Dahlén, Fabienne Levi-Acobas, Tom Baladi, Ivo Sarac, L. Marcus Wilhelmsson

Publikováno v: Journal of the American Chemical Society
Journal of the American Chemical Society, 2021, 143 (14), pp.5413-5424. ⟨10.1021/jacs.1c00014⟩
Journal of the American Chemical Society, American Chemical Society, 2021, 143 (14), pp.5413-5424. ⟨10.1021/jacs.1c00014⟩

International audience; Methods for tracking RNA molecules inside living cells without perturbing their natural interactions and functions are critical within biology and, in particular, to facilitate studies of therapeutic RNA delivery. We present a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b7d2187355619e1957adc2defc6f7db
https://doi.org/10.1101/2020.07.01.172767

Association of Autofluorescence-Based Detection of the Parathyroid Glands During Total Thyroidectomy With Postoperative Hypocalcemia Risk : Results of the PARAFLUO Multicenter Randomized Clinical Trial

Autor: Nicolas Turrin, Fares Benmiloud, Laurent Chiche, Régis Gras, Nathalie Chereau, Gaelle Godiris-Petit, Séverine Noullet, Jean-Charles Gillot, Stanislas Rebaudet, Guillaume Penaranda, Jean Gaudart

Publikováno v: JAMA Surgery
JAMA Surgery, American Medical Association, 2020, 155 (2), pp.106. ⟨10.1001/jamasurg.2019.4613⟩

International audience; Importance: Because inadvertent damage of parathyroid glands can lead to postoperative hypocalcemia, their identification and preservation, which can be challenging, are pivotal during total thyroidectomy.Objective: To determi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a0e8b605bd20e43fcae320d29a6e983
https://www.hal.inserm.fr/inserm-03026078

Optimization of Advanced Live-Cell Imaging through Red/Near-Infrared Dye Labeling and Fluorescence Lifetime-Based Strategies

Autor: Komuro, Yutaro, Galas, Ludovic, Morozov, Yury, Fahrion, Jennifer, Raoult, Emilie, Lebon, Alexis, Tilot, Amanda, Kikuchi, Shin, Ohno, Nobuhiko, Vaudry, David, Rakic, Pasko, Komuro, Hitoshi

Publikováno v: International Journal of Molecular Sciences
International Journal of Molecular Sciences, 2021, 22 (20), pp.11092. ⟨10.3390/ijms222011092⟩
Volume 22
Issue 20
International Journal of Molecular Sciences, Vol 22, Iss 11092, p 11092 (2021)

Fluorescence microscopy is essential for a detailed understanding of cellular processes
however, live-cell preservation during imaging is a matter of debate. In this study, we proposed a guide to optimize advanced light microscopy approaches by

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c98400f83e27a48f2bef19ad54e3d2f7
https://doi.org/10.3390/ijms222011092

In Vivo Monitoring of Calpain Activity by Forster Resonance Energy Transfer

Autor: Israeli, David, Cosette, Jérémie, Corre, Guillaume, Amor, Fatima, Poupiot, Jérôme, Montus, Marie, Gjata, Bernard, Hoch, Lucile, Henriques, Sara, Bruge, Celine, Marsolier, Justine, Benabides, Manon, Bourg, Nathalie, Tournois, Johana, Mahé, Gurvan, Morizur, Lise, Jarrige, Margot, Bigot, Anne, Nissan, Xavier, Lostal, William, Stockholm, Daniel, Richard, Isabelle

Publikováno v: Calpain: Methods and Protocols, Methods in Molecular Biology
Calpain: Methods and Protocols, Methods in Molecular Biology, pp.57-66, 2019, ⟨10.1007/978-1-4939-8988-1_5⟩
Methods Mol Biol.
Methods Mol Biol., 1915 (1), pp.57-66, 2019, ⟨10.1007/978-1-4939-8988-1_5⟩
Methods in Molecular Biology ISBN: 9781493989874

International audience; Calpains are a 15-member class of calcium-activated nonlysosomal neutral proteases. They are involved in many cellular processes and are highly upregulated in pathological conditions. Some are ubiquitously expressed (CAPN1, CA

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c9090e711d836d66797360ffea454b1b
https://hal.archives-ouvertes.fr/hal-03097121