The genomic copy number determination of improved Cre in transgenic mice using standards derived from megaprimer PCR constructed tandem repeats

Autor: Ge Diao, Min Tian, Runbo Li, Jie Huang, Jian Han, Jianxin Guo

Publikováno v: All Life, Vol 17, Iss 1 (2024)

Determination of the genomic copy number of Cre transgene is critical for the establishment of transgenic animal models. We demonstrate a real-time quantitative polymerase chain reaction (RT-qPCR) based method for genomic copy number determination of

Externí odkaz: https://doaj.org/article/60dccc766b2d42349721418f13dcbb07

Engineering a thermostable iron superoxide dismutase based on manganese superoxide dismutase from Thermus thermophilus

Autor: Li, Hui ^a, Feng, Zhi-mei ^a, Sun, Ya-jie ^a, Ning, Shou-jiao ^a, Zhou, Wan-long ^a, Liu, Ao ^a, Pan, Fang ^b, Zhao, Xiu-bo ^b, Zhu, Hu ^{a, ⁎}, Lu, Jian-R. ^{b, ⁎⁎}

Publikováno v: In Process Biochemistry January 2016 51(1):39-47

Recombination-assisted megaprimer (RAM) cloning

Autor: Mathieu, Jacques ^⁎, Alvarez, Emilia, Alvarez, Pedro J.J.

Publikováno v: In MethodsX 2014 1:23-29

Aislamiento de genes concretos de una genoteca

Autor: Regueira Amara, María Soledad

Publikováno v: RUC. Repositorio da Universidade da Coruña
Universidad de Alicante (UA)

[Resumen]: Las librerías genómicas o genotecas contienen el genoma completo de un organismo determinado, digerido en fragmentos de distinta longitud e insertado en vectores de clonación, siendo un recurso continuo para el estudio de genes concreto

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::8a3a1d95cc08269d210bd82e637c2a49
https://hdl.handle.net/2183/29263

An Optimized Metabarcoding Method for Mimiviridae

Autor: Keizo Nagasaki, Tetsuya Hayashi, Takashi Yoshida, Florian Prodinger, Hiroyuki Ogata, Yoshihito Takano, Romain Blanc-Mathieu, Kento Tominaga, Hisashi Endo, Yanze Li, Yasuhiro Gotoh, Daichi Morimoto, Kimiho Omae

Publikováno v: Microorganisms
Microorganisms, MDPI, 2020, 8 (4), pp.506. ⟨10.3390/microorganisms8040506⟩
Microorganisms, Vol 8, Iss 506, p 506 (2020)
Microorganisms, 2020, 8 (4), pp.506. ⟨10.3390/microorganisms8040506⟩
Volume 8
Issue 4

Mimiviridae is a group of viruses with large genomes and virions. Ecological relevance of Mimiviridae in marine environments has been increasingly recognized through the discoveries of novel isolates and metagenomic studies. To facilitate ecological

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98b797f70a4087c2fee66243c7f17a60
https://doi.org/10.3390/microorganisms8040506