Human iPSC Derived Enamel Organoid Guided by Single-Cell Atlas of Human Tooth Development

Autor: A Alghadeer, S Hanson-Drury, D Ehnes, YT Zhao, AP Patni, D O’Day, CH Spurrell, AA Gogate, A Phal, H Zhang, A Devi, Y Wang, L Starita, D Doherty, I Glass, J Shendure, D Baker, MC Regier, J Mathieu, H Ruohola-Baker

Tooth enamel secreted by ameloblasts is the hardest material in the human body, acting as a shield protecting the teeth. However, the enamel is gradually damaged or partially lost in over 90% of adults and cannot be regenerated due to a lack of amelo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::25f2862a8f66fe6c67ea0338aa32e5e9
https://doi.org/10.1101/2022.08.09.503399

Disruption of perinatal myeloid niches impacts the aging clock of pancreatic β cells.

Autor: O'Sell J; Department of Medicine, Diabetes Institute, and Institute of Stem Cells and Regenerative Medicine, University of Washington, Seattle WA 98109, USA., Cirulli V; Department of Medicine, Diabetes Institute, and Institute of Stem Cells and Regenerative Medicine, University of Washington, Seattle WA 98109, USA., Pardike S; Department of Medicine, Diabetes Institute, and Institute of Stem Cells and Regenerative Medicine, University of Washington, Seattle WA 98109, USA., Aare-Bentsen M; Department of Medicine, Diabetes Institute, and Institute of Stem Cells and Regenerative Medicine, University of Washington, Seattle WA 98109, USA., Sdek P; Department of Medicine, Diabetes Institute, and Institute of Stem Cells and Regenerative Medicine, University of Washington, Seattle WA 98109, USA., Anderson J; Department of Medicine, Diabetes Institute, and Institute of Stem Cells and Regenerative Medicine, University of Washington, Seattle WA 98109, USA., Hailey DW; Department of Laboratory Medicine and Pathology, and Institute of Stem Cells and Regenerative Medicine, University of Washington, Seattle WA 98109, USA., Regier MC; Institute of Stem Cells and Regenerative Medicine, University of Washington, Seattle WA 98109, USA., Gharib SA; Computational Medicine Core at Center for Lung Biology, Division of Pulmonary, Critical Care and Sleep Medicine, University of Washington, Seattle, WA 98109, USA., Crisa L; Department of Medicine, Diabetes Institute, and Institute of Stem Cells and Regenerative Medicine, University of Washington, Seattle WA 98109, USA.

Publikováno v: IScience [iScience] 2024 Aug 07; Vol. 27 (9), pp. 110644. Date of Electronic Publication: 2024 Aug 07 (Print Publication: 2024).

Single-cell census of human tooth development enables generation of human enamel.

Autor: Alghadeer A; Department of Biomedical Dental Sciences, Imam Abdulrahman bin Faisal University, College of Dentistry, Dammam 31441, Saudi Arabia; Department of Oral Health Sciences University of Washington, School of Dentistry, Seattle, WA 98109, USA; Department of Biochemistry, University of Washington School of Medicine, Seattle, WA 98195, USA; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109, USA., Hanson-Drury S; Department of Oral Health Sciences University of Washington, School of Dentistry, Seattle, WA 98109, USA; Department of Biochemistry, University of Washington School of Medicine, Seattle, WA 98195, USA; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109, USA., Patni AP; Department of Oral Health Sciences University of Washington, School of Dentistry, Seattle, WA 98109, USA; Department of Biochemistry, University of Washington School of Medicine, Seattle, WA 98195, USA; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109, USA; Cancer Biology and Stem Cell Biology Laboratory, Department of Genetic Engineering, School of Bioengineering, College of Engineering and Technology, SRM Institute of Science and Technology, Chennai 603203, India., Ehnes DD; Department of Biochemistry, University of Washington School of Medicine, Seattle, WA 98195, USA; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109, USA., Zhao YT; Department of Oral Health Sciences University of Washington, School of Dentistry, Seattle, WA 98109, USA; Department of Biochemistry, University of Washington School of Medicine, Seattle, WA 98195, USA; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109, USA., Li Z; Department of Biochemistry, University of Washington School of Medicine, Seattle, WA 98195, USA; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109, USA., Phal A; Department of Biochemistry, University of Washington School of Medicine, Seattle, WA 98195, USA; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109, USA; Department of Bioengineering, University of Washington, Seattle, WA 98195, USA., Vincent T; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109, USA; Department of Bioengineering, University of Washington, Seattle, WA 98195, USA., Lim YC; Department of Biochemistry, University of Washington School of Medicine, Seattle, WA 98195, USA; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109, USA., O'Day D; Department of Pediatrics, University of Washington, Seattle, WA 98195, USA; Brotman Baty Institute for Precision Medicine, Seattle, WA 98195, USA., Spurrell CH; Brotman Baty Institute for Precision Medicine, Seattle, WA 98195, USA., Gogate AA; Brotman Baty Institute for Precision Medicine, Seattle, WA 98195, USA; Seattle Children's Research Institute, Seattle, WA 98195, USA., Zhang H; Department of Restorative Dentistry, University of Washington, School of Dentistry, Seattle, WA 98195, USA., Devi A; Cancer Biology and Stem Cell Biology Laboratory, Department of Genetic Engineering, School of Bioengineering, College of Engineering and Technology, SRM Institute of Science and Technology, Chennai 603203, India., Wang Y; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109, USA; Paul G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA 98195, USA., Starita L; Brotman Baty Institute for Precision Medicine, Seattle, WA 98195, USA; Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA., Doherty D; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109, USA; Department of Pediatrics, University of Washington, Seattle, WA 98195, USA; Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, WA 98195, USA., Glass IA; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109, USA; Department of Pediatrics, University of Washington, Seattle, WA 98195, USA; Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, WA 98195, USA., Shendure J; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109, USA; Brotman Baty Institute for Precision Medicine, Seattle, WA 98195, USA; Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA., Freedman BS; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109, USA; Department of Bioengineering, University of Washington, Seattle, WA 98195, USA; Division of Nephrology, Department of Medicine, University of Washington School of Medicine, Seattle WA 98109., Baker D; Department of Biochemistry, University of Washington School of Medicine, Seattle, WA 98195, USA; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109, USA; Brotman Baty Institute for Precision Medicine, Seattle, WA 98195, USA; Department of Bioengineering, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Regier MC; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109, USA., Mathieu J; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109, USA; Department of Comparative Medicine, University of Washington School of Medicine, Seattle, WA 98195, USA., Ruohola-Baker H; Department of Biomedical Dental Sciences, Imam Abdulrahman bin Faisal University, College of Dentistry, Dammam 31441, Saudi Arabia; Department of Oral Health Sciences University of Washington, School of Dentistry, Seattle, WA 98109, USA; Department of Biochemistry, University of Washington School of Medicine, Seattle, WA 98195, USA; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109, USA; Brotman Baty Institute for Precision Medicine, Seattle, WA 98195, USA; Department of Bioengineering, University of Washington, Seattle, WA 98195, USA. Electronic address: hannele@uw.edu.

Publikováno v: Developmental cell [Dev Cell] 2023 Oct 23; Vol. 58 (20), pp. 2163-2180.e9. Date of Electronic Publication: 2023 Aug 14.

Embryo-scale, single-cell spatial transcriptomics.

Autor: Srivatsan SR; Department of Genome Sciences, University of Washington, Seattle, WA, USA., Regier MC; Department of Bioengineering. University of Washington, Seattle, WA, USA.; Institute for Stem Cell and Regenerative Medicine, Seattle, WA, USA., Barkan E; Department of Genome Sciences, University of Washington, Seattle, WA, USA.; Molecular and Cellular Biology Program, University of Washington, Seattle, WA, USA., Franks JM; Department of Genome Sciences, University of Washington, Seattle, WA, USA., Packer JS; Foresite Labs, Boston, MA, USA., Grosjean P; Department of Bioengineering. University of Washington, Seattle, WA, USA., Duran M; Department of Genome Sciences, University of Washington, Seattle, WA, USA., Saxton S; Department of Bioengineering. University of Washington, Seattle, WA, USA., Ladd JJ; Translational Research Program, Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA, USA., Spielmann M; Institute of Human Genetics, University of Lübeck, Lübeck, Germany.; Human Molecular Genomics Group, Max Planck Institute for Molecular Genetics, Berlin, Germany., Lois C; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA., Lampe PD; Translational Research Program, Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA, USA., Shendure J; Department of Genome Sciences, University of Washington, Seattle, WA, USA. shendure@uw.edu ksteve@uw.edu coletrap@uw.edu.; Allen Discovery Center for Cell Lineage Tracing, Seattle, WA, USA.; Howard Hughes Medical Institute, University of Washington, Seattle, WA, USA.; Brotman Baty Institute for Precision Medicine, Seattle, WA, USA., Stevens KR; Department of Bioengineering. University of Washington, Seattle, WA, USA. shendure@uw.edu ksteve@uw.edu coletrap@uw.edu.; Institute for Stem Cell and Regenerative Medicine, Seattle, WA, USA.; Brotman Baty Institute for Precision Medicine, Seattle, WA, USA.; Department of Laboratory Medicine and Pathology, Seattle, WA, USA., Trapnell C; Department of Genome Sciences, University of Washington, Seattle, WA, USA. shendure@uw.edu ksteve@uw.edu coletrap@uw.edu.; Allen Discovery Center for Cell Lineage Tracing, Seattle, WA, USA.; Brotman Baty Institute for Precision Medicine, Seattle, WA, USA.

Publikováno v: Science (New York, N.Y.) [Science] 2021 Jul 02; Vol. 373 (6550), pp. 111-117.

A Rainbow Reporter Tracks Single Cells and Reveals Heterogeneous Cellular Dynamics among Pluripotent Stem Cells and Their Differentiated Derivatives.

Autor: El-Nachef D; Department of Pathology, University of Washington, Seattle, WA 98109, USA; The Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA 98109, USA; Center for Cardiovascular Biology, University of Washington, Seattle, WA 98109, USA., Shi K; Department of Pathology, University of Washington, Seattle, WA 98109, USA., Beussman KM; The Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA 98109, USA; Department of Bioengineering, University of Washington, Seattle, WA 98105, USA; Department of Mechanical Engineering, University of Washington, Seattle, WA 98195, USA; Center for Cardiovascular Biology, University of Washington, Seattle, WA 98109, USA., Martinez R; Department of Pathology, University of Washington, Seattle, WA 98109, USA; The Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA 98109, USA., Regier MC; The Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA 98109, USA; Department of Bioengineering, University of Washington, Seattle, WA 98105, USA; Center for Cardiovascular Biology, University of Washington, Seattle, WA 98109, USA., Everett GW; The Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA 98109, USA; Department of Bioengineering, University of Washington, Seattle, WA 98105, USA; Center for Cardiovascular Biology, University of Washington, Seattle, WA 98109, USA., Murry CE; Department of Pathology, University of Washington, Seattle, WA 98109, USA; The Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA 98109, USA; Department of Bioengineering, University of Washington, Seattle, WA 98105, USA; Department of Medicine, Cardiology, University of Washington, Seattle, WA 98195, USA; Center for Cardiovascular Biology, University of Washington, Seattle, WA 98109, USA., Stevens KR; Department of Pathology, University of Washington, Seattle, WA 98109, USA; The Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA 98109, USA; Department of Bioengineering, University of Washington, Seattle, WA 98105, USA; Center for Cardiovascular Biology, University of Washington, Seattle, WA 98109, USA., Young JE; Department of Pathology, University of Washington, Seattle, WA 98109, USA; The Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA 98109, USA., Sniadecki NJ; The Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA 98109, USA; Department of Bioengineering, University of Washington, Seattle, WA 98105, USA; Department of Mechanical Engineering, University of Washington, Seattle, WA 98195, USA; Center for Cardiovascular Biology, University of Washington, Seattle, WA 98109, USA., Davis J; Department of Pathology, University of Washington, Seattle, WA 98109, USA; The Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA 98109, USA; Department of Bioengineering, University of Washington, Seattle, WA 98105, USA; Center for Cardiovascular Biology, University of Washington, Seattle, WA 98109, USA. Electronic address: jendavis@uw.edu.

Publikováno v: Stem cell reports [Stem Cell Reports] 2020 Jul 14; Vol. 15 (1), pp. 226-241. Date of Electronic Publication: 2020 Jul 02.