Zobrazeno 1 - 10
of 37
pro vyhledávání: '"MARK E. GUSTAFSON"'
Autor:
Alan K. Hunter, Natraj Ramasubramanyan, Mark E. Gustafson, Eric J. Suda, Paul Mensah, Thomas Kristin Elizabeth, Timothy M. Pabst
Publikováno v:
Journal of Chromatography A. 1216:7950-7956
This work provides a broad survey of binding and elution behavior of proteins on strong cation exchangers. Four proteins comprising two monoclonal antibodies, lysozyme, and cytochrome c were used as models in the investigation. Seven chromatography r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b36d8ab83e4da0604e21e25c262b5232
https://doi.org/10.1016/j.chroma.2009.09.040
https://doi.org/10.1016/j.chroma.2009.09.040
Publikováno v:
Biotechnology and Applied Biochemistry. 54:85-92
Optimization of refolding conditions for progenipoietin was performed. The molecule has five disulfide bonds and, hence, is a challenge to refold. Variables studied included pH, DTT (dithiothreitol) concentration, cystine concentration, urea concentr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54bbd8baad318d47dd81fcdee5f0d78b
https://doi.org/10.1042/ba20080268
https://doi.org/10.1042/ba20080268
Autor:
Eric J. Suda, Thomas Kristin Elizabeth, Mark E. Gustafson, Natraj Ramasubramanyan, Paul Mensah, Alan K. Hunter, Timothy M. Pabst
Publikováno v:
Journal of Chromatography A. 1216:5256-5264
The control of aggregate levels in recombinant protein based drugs is a primary concern during process development and manufacture. In recent years, a novel class of dextran-grafted ion exchange matrices has gained popularity for process scale protei
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1190d673f560ba85c61a92b4a8a3c347
https://doi.org/10.1016/j.chroma.2009.05.021
https://doi.org/10.1016/j.chroma.2009.05.021
Autor:
Natrajan Ramasubramanyan, Paul Mensah, Mark E. Gustafson, Alan K. Hunter, Timothy M. Pabst, Giorgio Carta
Publikováno v:
Biotechnology Progress. 24:1096-1106
This work concerns the chromatographic separation of protein charge variants using pH gradients generated by step changes in buffer composition with weak base anion exchange columns. A local equilibrium model is first developed to describe pH transit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd0c3a90cb9de7f347eacfcbec84a2d1
https://doi.org/10.1002/btpr.53
https://doi.org/10.1002/btpr.53
Publikováno v:
Journal of Chromatography A. 1202:107-110
This work demonstrates that cyclohexanedimethanol is an effective nonflammable organic solvent for the pilot scale reversed phase chromatography of recombinant apolipoprotein A-I(Milano). Cyclohexanedimethanol has low viscosity in water, enabling the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cef7200d000e951405b623674790a9d4
https://doi.org/10.1016/j.chroma.2008.06.032
https://doi.org/10.1016/j.chroma.2008.06.032
Autor:
Judy A Diaz-Collier, Mark O Palmier, Kuniko K Kretzmer, Bruce F Bishop, Rodney G Combs, Mark G Obukowicz, Ronald B Frazier, Gary S Bild, William D Joy, Steven R Hill, Kevin L Duffin, Mark E Gustafson, Kurt D Junger, Roy W Grabner, Gerald R Galluppi, Tze-Chein Wun
Publikováno v:
Thrombosis and Haemostasis. 71:339-346
SummaryHuman tissue factor pathway inhibitor (TFPI) was expressed in E. coli as a non-glycosylated protein with an additional alanine attached to the aminoterminus of the wild type molecule. High-level expression was obtained with pMON6875, a plasmid
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d487d2cf3fa01b602b11748ce7d00869
https://doi.org/10.1055/s-0038-1642440
https://doi.org/10.1055/s-0038-1642440
Autor:
Tze-Chein Wun, Kuniko K Kretzmer, Mark O Palmier, Kathleen C Day, Margaret D Huang, Dean J Welsch, Charles Lewis, Richard A Wolfe, James F Zobel, Gary W Lange, Ronald B Frazier, Gary S Bild, Michelle A Peel, Robert E Shell, Nancy A Horn, Kurt D Junger, Barbara A Foy, Mark E Gustafson, Richard M Leimgruber, William F Novotny, George J Broze, Yvonne E Pyla, Paul J Hippenmeyer, Thomas G Warren
Publikováno v:
Thrombosis and Haemostasis. 68:054-059
SummaryRecombinant tissue factor pathway inhibitor (rTFPI) has been expressed in four mammalian expression systems using human SK hepatoma, mouse C127, baby hamster kidney (BHK), and Chinese hamster ovary (CHO) cells as hosts. On sodium dodecyl sulfa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1d63389b2c57bc358d4345af1819cf2f
https://doi.org/10.1055/s-0038-1656317
https://doi.org/10.1055/s-0038-1656317
Autor:
Eric J. Suda, Sa V. Ho, Robert L. Dufield, Xing Wang, Alan K. Hunter, Robert E. Shell, Mark E. Gustafson, Ned M. Mozier, John T. Herberg, Thomas Kristin Elizabeth
Publikováno v:
Biotechnology progress. 25(2)
We have shown how product associating E. coli host cell proteins (HCPs) OppA and DppA can be substantially separated from apolipoprotein A-I(Milano) (apo A-I(M)) using Butyl Sepharose hydrophobic interaction chromatography (HIC). This work illustrate
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::091ab56ebf84da55a45b5b5e1d2cbabc
https://pubmed.ncbi.nlm.nih.gov/19291803
https://pubmed.ncbi.nlm.nih.gov/19291803
Autor:
Alan K. Hunter, Sa V. Ho, Robert E. Shell, Eric J. Suda, John T. Herberg, Mark E. Gustafson, Thomas Kristin Elizabeth
Publikováno v:
Journal of chromatography. A. 1204(1)
We have shown how protein self-association impacts the ion-exchange separation of modified forms and aggregates for apolipoprotein A-I(Milano). It is well known that reversible self-association of a protein can lead to chromatographic band broadening
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22eb02df86f8bce2acaa8857d6fec897
https://pubmed.ncbi.nlm.nih.gov/18701112
https://pubmed.ncbi.nlm.nih.gov/18701112
Autor:
Felipe A. Donate, Jefry E. Shields, Jeffrey Allen, Mark E. Gustafson, Yuk Chun Chiu, Anant Patkar, Timothy C. Frank
Publikováno v:
Biotechnology progress. 23(5)
Genetic modification of Gram-negative bacteria to express a desired protein within the cell's periplasmic space, located between the inner cytoplasmic membrane and the outer cell wall, can offer an attractive strategy for commercial production of the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b85a30eedeadfdbdf94938b54a4ca86
https://pubmed.ncbi.nlm.nih.gov/17760459
https://pubmed.ncbi.nlm.nih.gov/17760459