ReaL-MGE is a tool for enhanced multiplex genome engineering and application to malonyl-CoA anabolism.

Autor: Zheng W; State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, P. R. China.; Suzhou Research Institute of Shandong University, Room607, Building B of NUSP, NO.388 Ruoshui Road, SIP, Suzhou, Jiangsu, P. R. China.; Shenzhen Research Institute of Shandong University, A301 Virtual University Park in South District of Shenzhen, Guangdong, P. R. China., Wang Y; State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, P. R. China., Cui J; State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, P. R. China., Guo G; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, P. R. China., Li Y; State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, P. R. China., Hou J; State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, P. R. China., Tu Q; State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, P. R. China., Yin Y; Yuelushan Laboratory, Changsha, China., Stewart F; State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, P. R. China. francis.stewart@tu-dresden.de.; Genomics, Biotechnology Center, Center for Molecular and Cellular Bioengineering, Technische Universität Dresden, Tatzberg 47-51, Dresden, Germany. francis.stewart@tu-dresden.de.; School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia. francis.stewart@tu-dresden.de., Zhang Y; State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, P. R. China. zhangyouming@sdu.edu.cn., Bian X; State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, P. R. China. bianxiaoying@sdu.edu.cn., Wang X; State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, P. R. China. wangxue@sdu.edu.cn.

Publikováno v: Nature communications [Nat Commun] 2024 Nov 12; Vol. 15 (1), pp. 9790. Date of Electronic Publication: 2024 Nov 12.

FASN regulates STING palmitoylation via malonyl-CoA in macrophages to alleviate sepsis-induced liver injury.

Autor: Kang J; Research Institute of General Surgery, Nanjing Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, PR China., Wu J; Department of General Surgery, BenQ Medical Center, The Affiliated BenQ Hospital of Nanjing Medical University, Nanjing, PR China; Jiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Affiliated Zhongda Hospital, School of Medicine, Southeast University, Nanjing, PR China., Liu Q; Department of General Surgery, Affiliated Zhongda Hospital, School of Medicine, Southeast University, Nanjing, PR China., Jiang H; Department of General Surgery, BenQ Medical Center, The Affiliated BenQ Hospital of Nanjing Medical University, Nanjing, PR China., Li W; Department of Emergency Surgery, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, PR China., Li Y; Research Institute of General Surgery, Nanjing Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, PR China., Li X; Research Institute of General Surgery, Nanjing Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, PR China., Ni C; Surgical Research Laboratory, BenQ Medical Center, The Affiliated BenQ Hospital of Nanjing Medical University, Nanjing, PR China., Wu L; Research Institute of General Surgery, Jinling Hospital, Nanjing Medical University, Nanjing, PR China., Liu M; The Core Laboratory, Nanjing BenQ Medical Center, The Affiliated BenQ Hospital of Nanjing Medical University, Nanjing, PR China., Liu H; Surgical Research Laboratory, BenQ Medical Center, The Affiliated BenQ Hospital of Nanjing Medical University, Nanjing, PR China., Deng L; Research Institute of General Surgery, Jinling Hospital, School of Medicine, Southeast University, Nanjing, China., Lin Z; Department of General Surgery, BenQ Medical Center, The Affiliated BenQ Hospital of Nanjing Medical University, Nanjing, PR China., Wu X; Research Institute of General Surgery, Nanjing Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, PR China. Electronic address: wuxiuwen@nju.edu.cn., Zhao Y; Department of General Surgery, BenQ Medical Center, The Affiliated BenQ Hospital of Nanjing Medical University, Nanjing, PR China. Electronic address: zhaoyun0562019@163.com., Ren J; Research Institute of General Surgery, Nanjing Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, PR China. Electronic address: jiananr@nju.edu.cn.

Publikováno v: Biochimica et biophysica acta. Molecular basis of disease [Biochim Biophys Acta Mol Basis Dis] 2024 Oct; Vol. 1870 (7), pp. 167299. Date of Electronic Publication: 2024 Jun 13.

Hepatic malonyl-CoA synthesis restrains gluconeogenesis by suppressing fat oxidation, pyruvate carboxylation, and amino acid availability.

Autor: Deja S; Center for Human Nutrition, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA; Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA., Fletcher JA; Center for Human Nutrition, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA; Department of Clinical Nutrition, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA., Kim CW; Center for Human Nutrition, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA., Kucejova B; Center for Human Nutrition, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA., Fu X; Center for Human Nutrition, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA; Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA., Mizerska M; Center for Human Nutrition, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA., Villegas M; Center for Human Nutrition, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA., Pudelko-Malik N; Center for Human Nutrition, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA; Department of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry, Wroclaw University of Science and Technology, Wroclaw, Poland., Browder N; Center for Human Nutrition, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA., Inigo-Vollmer M; Center for Human Nutrition, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA., Menezes CJ; Children's Research Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA., Mishra P; Children's Research Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA., Berglund ED; Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA., Browning JD; Department of Clinical Nutrition, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA; Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA., Thyfault JP; Departments of Cell Biology and Physiology, Internal Medicine and KU Diabetes Institute, Kansas Medical Center, Kansas City, KS, USA., Young JD; Department of Chemical and Biomolecular Engineering, Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37235, USA., Horton JD; Center for Human Nutrition, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA; Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA; Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA. Electronic address: jay.horton@utsouthwestern.edu., Burgess SC; Center for Human Nutrition, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA; Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA. Electronic address: shawn.burgess@utsouthwestern.edu.

Publikováno v: Cell metabolism [Cell Metab] 2024 May 07; Vol. 36 (5), pp. 1088-1104.e12. Date of Electronic Publication: 2024 Mar 05.

Fatty acid elongation regulates mitochondrial β-oxidation and cell viability in prostate cancer by controlling malonyl-CoA levels.

Autor: Scott JS; South Australian ImmunoGENomics Cancer Institute, Adelaide Medical School and Freemasons Centre for Male Health and Wellbeing, University of Adelaide, Adelaide, SA, 5005, Australia; Precision Cancer Medicine Theme, South Australian Health and Medical Research Institute, Adelaide, SA, 5000, Australia., Quek LE; School of Mathematics and Statistics, The University of Sydney, Sydney, NSW, 2006, Australia., Hoy AJ; School of Medical Sciences, Charles Perkins Centre, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, 2006, Australia., Swinnen JV; LKI - Leuven Cancer Institute, Department of Oncology, Laboratory of Lipid Metabolism and Cancer, KU Leuven, Leuven, B-3000, Belgium., Nassar ZD; South Australian ImmunoGENomics Cancer Institute, Adelaide Medical School and Freemasons Centre for Male Health and Wellbeing, University of Adelaide, Adelaide, SA, 5005, Australia; Precision Cancer Medicine Theme, South Australian Health and Medical Research Institute, Adelaide, SA, 5000, Australia., Butler LM; South Australian ImmunoGENomics Cancer Institute, Adelaide Medical School and Freemasons Centre for Male Health and Wellbeing, University of Adelaide, Adelaide, SA, 5005, Australia; Precision Cancer Medicine Theme, South Australian Health and Medical Research Institute, Adelaide, SA, 5000, Australia. Electronic address: lisa.butler@adelaide.edu.au.

Publikováno v: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2024 Jan 08; Vol. 691, pp. 149273. Date of Electronic Publication: 2023 Nov 18.

Engineering of a Malonyl-CoA Ligase for Production of Fluorinated Polyketide Extender Units.

Autor: Paulsel TQ; Department of Chemistry, NC State University, 2620 Yarbrough Drive, Raleigh, NC 27607, USA.; Comparative Medicine Institute, NC State University, 1001 William Moore Drive, Raleigh, NC 27606, USA., Williams GJ; Department of Chemistry, NC State University, 2620 Yarbrough Drive, Raleigh, NC 27607, USA.; Comparative Medicine Institute, NC State University, 1001 William Moore Drive, Raleigh, NC 27606, USA.

Publikováno v: Chembiochem : a European journal of chemical biology [Chembiochem] 2024 Nov 04; Vol. 25 (21), pp. e202400532. Date of Electronic Publication: 2024 Sep 09.