Zobrazeno 1 - 10
of 436
pro vyhledávání: '"M.F Jayle"'
Publikováno v:
Clinica Chimica Acta. 59:147-154
A semi-automated determination of 17-oxosteroids in urinary extracts is described using a modified Zimmerman reaction in aqueous phase according to Epstein. In order to eliminate the interfering chromogens a double manifold and a double-beam colorime
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure. 533:408-414
The interaction of bovine serum albumin and rat alpha1-fetoprotein with aflatoxin B1 has been followed by the fluorescence quenching of the protein in presence of the ligand. The binding parameters (n, number of sites and Kd, dissociation constant) h
Autor:
M.F. Jayle, J.R. Pasqualini
Publikováno v:
Journal of Chromatography A. 5:336-340
A method for the separation of mono- and diacetates of di- and trioxygenated steroids is described. This procedure involves chromatographic adsorption on paper with less polar solvents. Of the steroids that have a C3 hydroxyl group it was possible to
Publikováno v:
Clinica Chimica Acta. 40:399-405
An original method is presented, allowing the estimation in urinary extracts of 5 to 100 μg of pregnanediol, in 4 h and with a high specificity. It involves rapid hydrolysis of Helix pomatia with β-glucuronidase and sulfatase and a single extractio
Publikováno v:
Clinica Chimica Acta. 16:409-416
Free hydroxyproline, acid hypro-peptides and alkaline hypro-peptides from human urine have been separated by Dowex 1 chromatography. Peptidic fractions are hydrolyzed with 6.0 N HCl, and hydroxyproline concentration evaluated in every fraction by a c
Publikováno v:
Clinica Chimica Acta. 33:45-50
Haptoglobin is prepared and purified from urine of nephrotic rats under stress by turpentine injection. Three techniques were successively used: Ammonium sulfate precipitation; Cation exchange chromatography; Gel filtration. The different steps of pu
Publikováno v:
Clinica Chimica Acta. 32:123-129
Resume We have observed haptoglobin in the urine of rats during Masugi's glomerulonephritis. Its proportion was correlated to that of proteinuria ( r = 0.82) but it appeared only if the proteinuria was higher than 12 g/1. The urinary proteins were ri
Publikováno v:
Clinica Chimica Acta. 8:538-546
A method for the determination of pregnanolone together with that of pregnanediol is described. This method uses chromatography on alumina, according to Klopper's technique. Pregnanolone is eluted in the first benzene eluates, containing 0.8% ethanol
Publikováno v:
Experientia. 30:193-195
Chez le rat, au cours de la polyarthrite par adjuvant, les courbes evolutives de l'orosomucoide et de l'haptoglobine serique presentent 2 pics importants paralleles a ceux du seromucoide et correspondant aux 2 phases inflammatoires de la maladie. Nou