Zobrazeno 1 - 10
of 40
pro vyhledávání: '"M. Wayne Davis"'
Autor:
M. Wayne Davis, Erik M. Jorgensen
Publikováno v:
Frontiers in Bioinformatics, Vol 2 (2022)
A plasmid Editor (ApE) is a free, multi-platform application for visualizing, designing, and presenting biologically relevant DNA sequences. ApE provides a flexible framework for annotating a sequence manually or using a user-defined library of featu
Externí odkaz:
https://doaj.org/article/44c514231b5041458de56f6bf87e81e0
Publikováno v:
PLoS Genetics, Vol 17, Iss 11 (2021)
Gene editing in C. elegans using plasmid-based CRISPR reagents requires microinjection of many animals to produce a single edit. Germline silencing of plasmid-borne Cas9 is a major cause of inefficient editing. Here, we present a set of C. elegans st
Externí odkaz:
https://doaj.org/article/dcfd7dde31fe417bae680c24232c6f3a
Publikováno v:
Frontiers in Synaptic Neuroscience, Vol 12 (2020)
The structural features of a synapse help determine its function. Synapses are extremely small and tightly packed with vesicles and other organelles. Visualizing synaptic structure requires imaging by electron microscopy, and the features in microgra
Externí odkaz:
https://doaj.org/article/8a1af260c2a24968814ea151815c57ed
Autor:
Maysam Mansouri, Itxaso Bellon-Echeverria, Aurélien Rizk, Zahra Ehsaei, Chiara Cianciolo Cosentino, Catarina S. Silva, Ye Xie, Frederick M. Boyce, M. Wayne Davis, Stephan C. F. Neuhauss, Verdon Taylor, Kurt Ballmer-Hofer, Imre Berger, Philipp Berger
Publikováno v:
Nature Communications, Vol 7, Iss 1, Pp 1-13 (2016)
Current viral gene delivery systems are limited in the amount of foreign DNA they can deliver to cells. Here the authors develop MultiPrime, a baculovirus-based vector system capable of multigene delivery into a wide variety of cells, and use Multipr
Externí odkaz:
https://doaj.org/article/91e7d81de6ed4df8941fdab73f25c864
Autor:
M. Wayne Davis, Erik M. Jorgensen
Publikováno v:
Frontiers in Bioinformatics, Vol 2 (2022)
A plasmid Editor (ApE) is a free, multi-platform application for visualizing, designing, and presenting biologically relevant DNA sequences. ApE provides a flexible framework for annotating a sequence manually or using a user-defined library of featu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::501c8f54a49a1e1e106287966356e233
https://pubmed.ncbi.nlm.nih.gov/36304290
https://pubmed.ncbi.nlm.nih.gov/36304290
Autor:
Shigeki Watanabe, Qiang Liu, M Wayne Davis, Gunther Hollopeter, Nikita Thomas, Nels B Jorgensen, Erik M Jorgensen
Publikováno v:
eLife, Vol 2 (2013)
Synaptic vesicles can be released at extremely high rates, which places an extraordinary demand on the recycling machinery. Previous ultrastructural studies of vesicle recycling were conducted in dissected preparations using an intense stimulation to
Externí odkaz:
https://doaj.org/article/3c9b8f7c312a4155964027368d6deb0d
Autor:
Ruipeng Guo, Soren Nelson, Matthew Regier, M. Wayne Davis, Erik M. Jorgensen, Jason Shepherd, Rajesh Menon
Publikováno v:
Opt Express
Deep-brain microscopy is strongly limited by the size of the imaging probe, both in terms of achievable resolution and potential trauma due to surgery. Here, we show that a segment of an ultra-thin multi-mode fiber (cannula) can replace the bulky mic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc82c964dcd8208e83dd66e6983aa731
https://doi.org/10.1364/oe.446241
https://doi.org/10.1364/oe.446241
Autor:
Shigeki Watanabe, Kristina Lippmann, M. Wayne Davis, Erik M. Jorgensen, Sumana Raychaudhuri, Grant F. Kusick, Morven Chin, Kadidia P. Adula, Edward J. Hujber, Thien N Vu
Publikováno v:
Nature neuroscience
Synaptic vesicles fuse with the plasma membrane to release neurotransmitter following an action potential, after which new vesicles must refill vacated release sites. How many vesicles can fuse at a single active zone, where they fuse within the acti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5eb1e54e96550cacb2ebcacadcb04bd1
https://doi.org/10.1101/509216
https://doi.org/10.1101/509216
Autor:
Christian Frøkjær-Jensen, Erik M. Jorgensen, Stephane Flibotte, Mihail Sarov, Matthew L. LaBella, Jon Taylor, M. Wayne Davis, Andrei Pozniakovsky, Donald G. Moerman
Publikováno v:
Nature Methods. 11:529-534
We have generated a recombinant Mos1 transposon that can insert up to 45-kb transgenes into the Caenorhabditis elegans genome. The minimal Mos1 transposon (miniMos) is 550 bp long and inserts DNA into the genome at high frequency (~60% of injected an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bd5e6ab96d76d5f5d523720ec39b62b5
https://doi.org/10.1038/nmeth.2889
https://doi.org/10.1038/nmeth.2889
Autor:
Berit Söhl-Kielczynski, Benjamin R. Rost, Erik M. Jorgensen, Christian Rosenmund, Marcial Camacho-Perez, Shigeki Watanabe, M. Wayne Davis
Publikováno v:
Nature
Nature 504(7479), 242-247 (2013). doi:10.1038/nature12809
Nature
To sustain neurotransmission, synaptic vesicles and their associated proteins must be recycled locally at synapses. Synaptic vesicles are thought to be regenerated approximately 20 s after fusion by the assembly of clathrin scaffolds or in approximat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab0a4e2b3f2ab9ff988f7b18eec2fd68
http://europepmc.org/articles/PMC3957339
http://europepmc.org/articles/PMC3957339