Zobrazeno 1 - 10
of 63
pro vyhledávání: '"M. Silberklang"'
Autor:
C. P. Chan, P. K. Tsai, C. Yu Lp, J. Irwin, D. K. Robinson, J. Tung, A. B. Lenny, M. Silberklang, T. C. Seamans, D. K. Lee
Publikováno v:
Biotechnology and Bioengineering. 44:727-735
Many mammalian cell fed-batch processes rely on maintaining the cells in a viable and productive state for extended periods of time in order to reach high final concentrations of secreted protein. In the work described herein, a nonamplified NSO cell
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4cd43e29cfb55478a68cb07ee35539ac
https://doi.org/10.1002/bit.260440609
https://doi.org/10.1002/bit.260440609
Autor:
Jack A. Alhadeff, Charlotte C Ip, G.E. Mark, L.H. Huang, Ronald W. Ellis, M. Silberklang, W.J. Miller, J. Zhu, J. Glushka, H. Vanhalbeek
Publikováno v:
Archives of Biochemistry and Biophysics. 308:387-399
This study characterized the N -glycans of a humanized immunoglobulin G 4 (IgG 4 ) expressed in NS/O mouse myeloma cells and directed against the CD18 family of adhesion-promoting receptors on leukocytes. The N -glycans were released from the purifie
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::487d9593f0cbe0ae78b862d7c82faac3
https://doi.org/10.1006/abbi.1994.1055
https://doi.org/10.1006/abbi.1994.1055
Autor:
A Talento, M Nguyen, S Law, J K Wu, M Poe, J T Blake, M Patel, T J Wu, C L Manyak, M Silberklang
Publikováno v:
The Journal of Immunology. 149:4009-4015
Murine CTL have seven serine proteases, known as granzymes, in their lytic granules. Despite considerable effort, convincing evidence that these enzymes play an obligatory role in the lytic process has not been presented. To investigate the function
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1b73518706a9936cf81404ec19e1e9a8
https://doi.org/10.4049/jimmunol.149.12.4009
https://doi.org/10.4049/jimmunol.149.12.4009
Autor:
Pieter C. Wensink, H.A. Hosbach, M. Silberklang, S Sharp, Dieter Söll, T. Schmidt, Donald B. DeFranco, J.P. Gergen, Eric Kubli
Publikováno v:
Nucleic Acids Research. 9:5867-5882
We have isolated four segments of Drosophila melanogaster DNA that hybridize to homologous initiator tRNAMet. Three of the cloned fragments contain initiator tRNA genes, each of which can be transcribed in vitro. The fourth clone, pPW568, contains an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d675598735ecb0639ae4f6398b69acf4
https://doi.org/10.1093/nar/9.22.5867
https://doi.org/10.1093/nar/9.22.5867
NOVEL SEQUENCING TECHNIQUES APPLIED TO THE 'tRNA-LIKE' 3′-TERMINUS OF TURNIP YELLOW MOSAIC VIRUS RNA
A fragment representing the 3′-terminal “tRNA-like” region of Turnip Yellow Mosaic Virus RNA has been isolated, and partially sequenced. Sequence information was derived entirely by in vitro [ 32 P] end-labelling techniques. The new methodologi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3f0e0e9de84988fbc068f7a8af727947
https://doi.org/10.1016/b978-0-12-077350-3.50041-1
https://doi.org/10.1016/b978-0-12-077350-3.50041-1
Autor:
Joyce E. Heckman, W E Barnett, Simon H. Chang, L I Hecker, C. K. Brum, Uttam L. RajBhandary, M. Silberklang, J. J. Schnabel
The nucleotide sequence of cytoplasmic phenylalanine tRNA from Euglena gracilis has been elucidated using procedures described previously for the corresponding chloroplastic tRNA [Cell, 9, 717 (1976)]. The sequence is: pG-C-C-G-A-C-U-U-A-m(2)G-C-U-Cm
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c4c0c06ca0468e5ca700e342201f3ff
https://europepmc.org/articles/PMC327342/
https://europepmc.org/articles/PMC327342/
Publikováno v:
Nucleic acids research. 4(12)
A method is described for the direct sequence analysis of 20-25 nucleotides from the termini of 5'- or 3'-end-group [32P] labeled RNA. The method involves partial endonucleolytic digestion of the labeled RNA with nuclease P1 (from Penicillium citrinu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cedeef6b20a4c8e41683b02ed2f3d9a6
https://pubmed.ncbi.nlm.nih.gov/202925
https://pubmed.ncbi.nlm.nih.gov/202925
Autor:
Steven D. Schwartzbach, Uttam L. RajBhandary, A.M. Gillum, L I Hecker, W E Barnett, M. Silberklang
Publikováno v:
Nucleic acids research. 4(12)
Initiator methionine tRNA from the cytoplasm of Neurospora crassa has been purified and sequenced. The sequence is: pAGCUGCAUm1GGCGCAGCGGAAGCGCM22GCY*GGGCUCAUt6AACCCGGAGm7GU (or D) - CACUCGAUCGm1AAACGAG*UUGCAGCUACCAOH. Similar to initiator tRNAs from
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aec1e9754a2f146d4bbec58fa124029d
https://pubmed.ncbi.nlm.nih.gov/146192
https://pubmed.ncbi.nlm.nih.gov/146192
We have found the rat pituitary cell line, GH3 to be an efficient host for expression of foreign genes, and especially productive with secreted proteins, such as bovine Growth Hormone (bGH). A more adherent cell subpopulation was first derived, which
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::df0ea8e6c8aebef5f9f85713343dbbac
https://doi.org/10.1016/b978-0-408-02732-8.50021-3
https://doi.org/10.1016/b978-0-408-02732-8.50021-3
Publisher Summary This chapter describes a general method, which has made possible the sequence analysis of tRNAs from mitochondria, chloroplasts, and a wide variety of prokaryotic and eukaryotic organisms using in some cases as little as 20μg of pu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7f70f5389aaa22e4e9d660aae074c898
https://doi.org/10.1016/0076-6879(79)59072-7
https://doi.org/10.1016/0076-6879(79)59072-7