Zobrazeno 1 - 10
of 323
pro vyhledávání: '"M. Ravazzola"'
Publikováno v:
Journal of Cell Science. 111:3081-3090
On the basis of the cell surface protein CD8 we have constructed reporter molecules for both anterograde and retrograde transport from the Golgi complex. The cytoplasmic tail of CD8 was exchanged by a construct comprising a hemagglutinin (HA) epitope
Autor:
Inbok Paek, Mylène Amherdt, Thomas H. Söllner, L Orci, Hediye Erdjument-Bromage, Paul Tempst, James E. Rothman, M Ravazzola
Publikováno v:
The Journal of Cell Biology
We report the identification and characterization of ERS-24 (Endoplasmic Reticulum SNARE of 24 kD), a new mammalian v-SNARE implicated in vesicular transport between the ER and the Golgi. ERS24 is incorporated into 20S docking and fusion particles an
Autor:
M Ravazzola, M Amherdt, F. Lottspeich, J B Helms, Felix T. Wieland, Martina Bremser, K Sohn, Klaus Fiedler, L. Orci
Publikováno v:
The Journal of Cell Biology
Formation of non-clathrin-coated vesicles requires the recruitment of several cytosolic factors to the Golgi membrane. To identify membrane proteins involved in this budding process, a highly abundant type I transmembrane protein (p23) was isolated f
Autor:
L Orci, Lynne Lacomis, Paul Tempst, Mylène Amherdt, M Ravazzola, M Nagahama, James E. Rothman, Thomas H. Söllner
Publikováno v:
The Journal of Cell Biology
We report the identification of a putative v-SNARE (GOS-28), localized primarily to transport vesicles at the terminal rims of Golgi stacks. In vitro, GOS-28, A Golgi SNARE of 28 kD, is efficiently packaged into Golgi-derived vesicles, which are most
Autor:
L Orci, H Plutner, Mylène Amherdt, C Dascher, G Tanigawa, M Ravazzola, James E. Rothman, William E. Balch, Osamu Kuge, T Rowe
Publikováno v:
The Journal of Cell Biology
Two new members (Sar1a and Sar1b) of the SAR1 gene family have been identified in mammalian cells. Using immunoelectron microscopy, Sar1 was found to be restricted to the transitional region where the protein was enriched 20-40-fold in vesicular carr
Autor:
L Orci, Gary Tanigawa, Mylène Amherdt, Sayuri Hara-Kuge, James E. Rothman, Felix T. Wieland, Osamu Kuge, M Ravazzola
Publikováno v:
The Journal of Cell Biology
cDNA encoding the 20-kD subunit of coatomer, zeta-COP, predicts a protein of 177-amino acid residues, similar in sequence to AP17 and AP19, subunits of the clathrin adaptor complexes. Polyclonal antibody directed to zeta-COP blocks the binding of coa
Publikováno v:
The Journal of Cell Biology
The cycle of nucleotide exchange and hydrolysis by a small GTP-binding protein, ADP-ribosylation factor (ARF), helps to provide vectoriality to vesicle transport. Coat assembly is triggered when ARF binds GTP, initiating transport vesicle budding, an
Autor:
M. Ohneda, J. H. Johnson, L. R. Inman, L. Chen, K. Suzuki, Y. Goto, T. Alam, M. Ravazzola, L. Orci, R. H. Unger
Publikováno v:
Diabetes. 42:1065-1072
Autor:
Tausif Alam, Ken Ichi Suzuki, Lindsey Inman, M. Ohneda, Roger H Unger, John H. Johnson, Ling Chen, Yoshio Goto, Lelio Orci, M. Ravazzola
Publikováno v:
Diabetes. 42:1065-1072
GLUT2 underexpression has been reported in the +-cells of Zucker diabetic fatty rats and db/db mice, models of spontaneously occurring NIDDM with antecedent obesity. To determine whether the +-cells of a nonobese rodent model of NIDDM exhibit the sam
Autor:
M Ravazzola, Jean-Dominique Vassalli, Alain Perrelet, Philippe A. Halban, Lelio Orci, M. Amherdt
Publikováno v:
The Journal of Cell Biology
The Journal of Cell Biology, Vol. 99, No 6 (1984) pp. 2187-2192
The Journal of Cell Biology, Vol. 99, No 6 (1984) pp. 2187-2192
The secretion of insulin by the pancreatic B-cell involves a passage of the newly synthetized (pro)insulin polypeptides across the Golgi apparatus, at the trans pole of which secretory proteins are released as a population of secretory granules chara