Výsledky vyhledávání - "M. P. Broekhuijsen"

A comprehensive study on the role of the Yersinia pestis virulence markers in an animal model of pneumonic plague

Autor: M. P. Broekhuijsen, Floris J. Bikker, D. van der Kleij, Wendy E. Kaman, N. J. Silman, S. Hawkey

Publikováno v: Folia Microbiologica
Folia Microbiologica, 56(2), 95-102. Springer Netherlands
Kaman, W E, Hawkey, S, van der Kleij, D, Broekhuijsen, M P, Silman, N J & Bikker, F J 2011, ' A comprehensive study on the role of the Yersinia pestis virulence markers in an animal model of pneumonic plague ', Folia Microbiologica, vol. 56, no. 2, pp. 95-102 . https://doi.org/10.1007/s12223-011-0027-z
Folia Microbiologica, 2, 56, 95-102
Folia Microbiologica, 56(2), 95-102. Nakladatelstvi Eskoslovenske Akademie V Image d
Folia microbiologica, 56(2), 95-102. Springer Netherlands

We determined the role of Yersinia pestis virulence markers in an animal model of pneumonic plague. Eleven strains of Y. pestis were characterized using PCR assays to detect the presence of known virulence genes both encoded by the three plasmids as

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53b0a211fbdf6c7ef730780929031238
https://hdl.handle.net/11245/1.347357

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The ER chaperone encoding bipA gene of black Aspergilli is induced by heat shock and unfolded proteins

Autor: C.A.M.J.J. van den Hondel, P.J. Punt, M. P. Broekhuijsen, A. Beijersbergen, A van 't Hoog, A Drint-Kuyvenhoven, I. A. van Gemeren, C. T. Verrips

Publikováno v: Gene. 198(1-2)

We describe the cloning and characterisation of the BiP gene homologues of the filamentous fungi Aspergillus niger and Aspergillus awamori. The BiP genes of these black Aspergilli encode an identical protein of 672 amino acids, which has a high homol

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::591929da52337497909ecc4c688a39c9
https://pubmed.ncbi.nlm.nih.gov/9370263

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Secretion of heterologous proteins by Aspergillus niger: production of active human interleukin-6 in a protease-deficient mutant by KEX2-like processing of a glucoamylase-hIL6 fusion protein

Autor: James R. Kinghorn, M. P. Broekhuijsen, Roland Contreras, I. E. Mattern, C.A.M.J.J. van den Hondel

Publikováno v: Journal of Biotechnology, 31, 135-145

To develop improved methods for heterologous protein production in Aspergillus niger, we studied the secretion of human interleukin-6 (hIL6). Since in vitro experiments with culture medium revealed that hIL6 was rapidly degraded, several protease-def

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24697ddfa0015ef721c4a7cf93edb32c
https://pubmed.ncbi.nlm.nih.gov/7764298

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The development of a homologous transformation system for Aspergillus oryzae based on the nitrate assimilation pathway: A convenient and general selection system for filamentous fungal transformation

Autor: Edward I. Campbell, Roland Contreras, James R. Kinghorn, Janet A. Macro, M. P. Broekhuijsen, Dirk Carrez, Yolanda M. J. T. de Ruiter-Jacobs, Shiela E. Unkles, Cees A. M. J. J. van den Hondel

Publikováno v: Molecular and General Genetics MGG. 218:99-104

The development of an efficient and homologous transformation system for Aspergillus oryzae is described. This is based on nitrate reductase (niaD) of the nitrate assimilation pathway. The niaD system offers a number of inherent advantages over many

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5ef52e5dee750fd2422419e0be620bed
https://doi.org/10.1007/bf00330571

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Synthesis of fusion proteins containing antigenic determinants of foot-and-mouth disease virus

Autor: Simon Barteling, Marijke J. Kottenhagen, Ton Blom, Rob H. Meloen, Betty E. Enger-Valk, Peter H. Pouwels, M. P. Broekhuijsen

Publikováno v: Vaccine. 4:119-124

Part of the genome of foot-and-mouth disease virus (FMDV) type 01,BFS, including the sequence encoding the capsid polypeptide VP1, was cloned in Escherichia coli following a new cloning strategy. The clone containing the VP1 sequence was used for the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ca47598c6c10cec9b17ec414a73875a
https://doi.org/10.1016/0264-410x(86)90050-2

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Synthesis of fusion proteins with multiple copies of an antigenic determinant of foot-and-mouth disease virus antibodies

Autor: P.H. Pouwels, E A Klasen, J. M. M. Van Rijn, Betty E. Enger-Valk, T Blom, M. P. Broekhuijsen, M J Fasbender

Publikováno v: Gene. 49:189-197

A series of four expression plasmids coding for fusion proteins containing foot-and-mouth disease virus (FMDV) sequences was constructed. The fusion proteins contain a large part of beta-galactosidase from Escherichia coli preceded (N-terminal) by 1,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::80ddaeda1f83cca86ab45230afbc139c
https://doi.org/10.1016/0378-1119(86)90279-9

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A gene transfer system based on the homologous pyrG gene and efficient expression of bacterial genes in Aspergillus oryzae

Autor: Cees A. M. J. J. van den Hondell, Roland Contreras, Yolanda M. J. T. de Ruiter-Jacobs, Peter H. Pouwels, Sheila E. Unkles, James R. Kinghorn, Edward I. Campbell, M. P. Broekhuijsen

Publikováno v: Current Genetics. 16:159-163

A homologous transformation system for Aspergillus oryzae is described. The system is based on an A. oryzae strain deficient in orotidine-5'-phosphate decarboxylase (pyrG) and the vector pAO4-2, which contains a functional A. oryzae pyrG gene as sele

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba04822629910da4068bfacefb75c0ee
https://doi.org/10.1007/bf00391472

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Fusion proteins with multiple copies of the major antigenic determinant of foot-and-mouth disease virus protect both the natural host and laboratory animals

Autor: Betty E. Enger-Valk, F. Brown, P.H. Pouwels, M. J. Francis, J. M. M. Van Rijn, A. J. M. Blom, M. P. Broekhuijsen

Publikováno v: The Journal of general virology. 68

Proteins consisting of one, two or four copies of the amino acid sequence 137 to 162, which contains the major immunogenic site of VP1 of foot-and-mouth disease virus, attached to the N-terminus of beta-galactosidase have been expressed in Escherichi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62f8fc96604c1d950930ca17b4e4b01c
https://pubmed.ncbi.nlm.nih.gov/2447225

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