Zobrazeno 1 - 10
of 61
pro vyhledávání: '"M. Mulqueen"'
Autor:
Hao Zhang, Ryan M. Mulqueen, Natalie Iannuzo, Dominique O. Farrera, Francesca Polverino, James J. Galligan, Julie G. Ledford, Andrew C. Adey, Darren A. Cusanovich
Publikováno v:
Genome Biology, Vol 25, Iss 1, Pp 1-41 (2024)
Abstract We develop a large-scale single-cell ATAC-seq method by combining Tn5-based pre-indexing with 10× Genomics barcoding, enabling the indexing of up to 200,000 nuclei across multiple samples in a single reaction. We profile 449,953 nuclei acro
Externí odkaz:
https://doaj.org/article/45dac0a06c854b82a3eca3eae9b2d359
Autor:
Ruth V. Nichols, Brendan L. O’Connell, Ryan M. Mulqueen, Jerushah Thomas, Ashley R. Woodfin, Sonia Acharya, Gail Mandel, Dmitry Pokholok, Frank J. Steemers, Andrew C. Adey
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-10 (2022)
Despite the importance of DNA methylation, accessible and high-throughput methods to profile methylation at the single-cell level are lacking. Here, the authors present sciMETv2, a high-throughput workflow that provides high-quality single-cell methy
Externí odkaz:
https://doaj.org/article/819c0be9d5a24f45850610d10e5f6cef
Autor:
Casey A. Thornton, Ryan M. Mulqueen, Kristof A. Torkenczy, Andrew Nishida, Eve G. Lowenstein, Andrew J. Fields, Frank J. Steemers, Wenri Zhang, Heather L. McConnell, Randy L. Woltjer, Anusha Mishra, Kevin M. Wright, Andrew C. Adey
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-16 (2021)
Spatial orientation of cells in an interconnected network is lost in high-throughput single-cell epigenomic assays. Here the authors present sciMAP-ATAC to produce spatially resolved single-cell ATAC-seq data.
Externí odkaz:
https://doaj.org/article/1ad12e0e2a2a4ba0b41d734d7e7f4e32
Autor:
Aysegul Ors, Alex Daniel Chitsazan, Aaron Reid Doe, Ryan M Mulqueen, Cigdem Ak, Yahong Wen, Syber Haverlack, Mithila Handu, Spandana Naldiga, Joshua C Saldivar, Hisham Mohammed
Publikováno v:
Nucleic Acids Research. 50:11492-11508
Breast cancers are known to be driven by the transcription factor estrogen receptor and its ligand estrogen. While the receptor's cis-binding elements are known to vary between tumors, heterogeneity of hormone signaling at a single-cell level is unkn
Autor:
Hao Zhang, Ryan M. Mulqueen, Natalie Iannuzo, Dominique O. Farrera, Francesca Polverino, James J. Galligan, Julie G. Ledford, Andrew C. Adey, Darren A. Cusanovich
Measuring chromatin accessibility is a powerful method to identify cell types and states. Performed at single-cell resolution, this assay has generated catalogs of genome-wide DNA regulatory sites, whole-organism cell atlases, and dynamic chromatin r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::442eda35c864de17b24ddb9f94d423b4
https://doi.org/10.1101/2023.05.11.540245
https://doi.org/10.1101/2023.05.11.540245
Autor:
Brendan O'Connell, Rosalie C. Sears, Frank J. Steemers, Brian J. O'Roak, Andrew Adey, Ryan M. Mulqueen, Casey A. Thornton, Galip Gürkan Yardımcı, Dmitry K. Pokholok, Jason Link, Fan Zhang
Publikováno v:
Nature biotechnology
Single-cell genomics assays have emerged as a dominant platform for interrogating complex biological systems. Methods to capture various properties at the single-cell level typically suffer a tradeoff between cell count and information content, which
Autor:
Heather L. McConnell, Andrew Adey, Randy Woltjer, Casey A. Thornton, Anusha Mishra, Frank J. Steemers, Kristof A. Torkenczy, Ryan M. Mulqueen, Eve G. Lowenstein, Kevin M. Wright, Andrew Nishida, Andrew J. Fields, Wenri Zhang
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-16 (2021)
Nature Communications
Nature Communications
High-throughput single-cell epigenomic assays can resolve cell type heterogeneity in complex tissues, however, spatial orientation is lost. Here, we present single-cell combinatorial indexing on Microbiopsies Assigned to Positions for the Assay for T
Autor:
Jay Shendure, Frank J. Steemers, Brian J. O'Roak, Cole Trapnell, Zheng Xia, Dmitry K. Pokholok, Andrew Adey, John R. Sinnamon, Ryan M. Mulqueen, Steven Norberg, Andrew J. Fields, Kristof A. Torkenczy, Duanchen Sun
Publikováno v:
Nature biotechnology
We present a highly scalable assay for whole-genome methylation profiling of single cells. We use our approach, single-cell combinatorial indexing for methylation analysis (sci-MET), to produce 3,282 single-cell bisulfite sequencing libraries and ach
Autor:
Jerome H. Taylor, Daniel Gabriel, Margot O Reed, Ewgeni Jakubovski, Angeli Landeros-Weisenberger, Jessica A. Johnson, Catherine G. Coughlin, Jilian M. Mulqueen, Michael H. Bloch
Publikováno v:
Neuropsychopharmacology. 43:325-333
Many patients with social anxiety disorder (SAD) experience inadequate symptom relief from available treatments. Ketamine is a potent N-methyl-D-aspartate receptor antagonist with a potentially novel mechanism of action for the treatment of anxiety d
Autor:
James F. Leckman, Jilian M. Mulqueen, Stephanie C. Cohen, Eduardo Ferracioli-Oda, Catherine G. Coughlin, Zachary D. Stuckelman, Michael H. Bloch
Publikováno v:
The Journal of Clinical Psychiatry. 78:e648-e655
Objective Irritability is listed as a common side effect of psychostimulant medications. However, psychostimulants have been demonstrated as an effective treatment in reducing irritability and aggression in children with attention-deficit/hyperactivi