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pro vyhledávání: '"M. Melanie Yelton"'
Autor:
Kellee A. Roberti, Edward J. Mullaney, John E. Hamer, William E. Timberlake, M. Melanie Yelton
Publikováno v:
Molecular and General Genetics MGG. 199:37-45
We have determined the structure and complete nucleotide sequence of the trifunctional trpC gene from the Ascomycetous fungus Aspergillus nidulans. Results from RNA gel blot analyses showed that this gene encodes two size classes of polyribosomal, po
Autor:
Duane C. Yoch, M. Melanie Yelton
Publikováno v:
Microbiology. 123:335-342
Glutamate synthase, an enzyme involved in glutamate biosynthesis in nitrogen-deficient environments, has been purified 200-fold from the photosynthetic bacterium Rhodospirillum rubrum. The in vivo association of this NADPH-linked glutamate synthase w
We constructed a 9.9-kilobase cloning vector, designated pKBY2, for isolating genes by complementation of mutations in Aspergillus nidulans . pKBY2 contains the bacteriophage λ cos site, to permit in vitro assembly of phage particles; a bacterial or
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9107f60ffaf6099c100e9cb7475236b3
https://europepmc.org/articles/PMC397141/
https://europepmc.org/articles/PMC397141/
We have cloned the trifunctional trpC gene from Aspergillus nidulans by hybrid phage lambda complementation of an Escherichia coli trpC mutant lacking phosphoribosylanthranilate isomerase activity. Four different phages sharing a 4.3-kilobase region
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2eb3d9c265c24e6e9b1eb15134d2a730
https://europepmc.org/articles/PMC534383/
https://europepmc.org/articles/PMC534383/
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 81(5)
We constructed a chimeric plasmid carrying a complete copy of the trifunctional trpC gene from the Ascomycete fungus Aspergillus nidulans. This plasmid, designated pHY201, replicates in Escherichia coli, where it confers resistance to ampicillin and