Výsledky vyhledávání - "M. K. Shigenaga"

Biomarkers of oxidative stress study III. Effects of the nonsteroidal anti-inflammatory agents indomethacin and meclofenamic acid on measurements of oxidative products of lipids in CCl4 poisoning

Publikováno v: Free radical biologymedicine. 38(6)

Plasma and urinary levels of malondialdehyde-like products (MDA) and isoprostanes were identified as markers of in vivo lipid peroxidation in an animal model of CCl4 poisoning. We sought to determine the extent to which the formation of these oxidati

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::40a7ae9e59ececc448887c8faaf16fb2
https://pubmed.ncbi.nlm.nih.gov/15721981

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Quantitation of protein-bound 3-nitrotyrosine by high-performance liquid chromatography with electrochemical detection

Autor: M K, Shigenaga

Publikováno v: Methods in enzymology. 301

HPLC with electrochemical detection of the N-acetylated, dithionite-reduced derivative of NTyr provides a highly sensitive and selective means of measuring this nitrated residue in biological samples. The detection of protein-bound NTyr at baseline l

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::afc4f8a77f06c1c243cd3372c5610d7f
https://pubmed.ncbi.nlm.nih.gov/9919551

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Assays of oxidative DNA damage biomarkers 8-oxo-2'-deoxyguanosine and 8-oxoguanine in nuclear DNA and biological fluids by high-performance liquid chromatography with electrochemical detection

Autor: M K, Shigenaga, E N, Aboujaoude, Q, Chen, B N, Ames

Publikováno v: Methods in enzymology. 234

High-performance liquid chromatography with electrochemical detection is a highly sensitive and selective method for detecting oxo8dG and oxo8Gua, biomarkers of oxidative DNA damage. When employed together with the DNA isolation and monoclonal antibo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::9cbdf6a62c707eb0b1eacd51a4d1c913
https://pubmed.ncbi.nlm.nih.gov/7808289

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Antioxidant Prevention of Birth Defects and Cancer

Autor: T. M. Hagen, C. G. Fraga, M. K. Shigenaga, Bruce N. Ames, P. Motchnik

Publikováno v: Male-Mediated Developmental Toxicity ISBN: 9781461357643

Metabolism, like other aspects of life, involves trade-offs. Damage to DNA and protein by oxidant by-products of normal metabolism is massive. We argue that this damage (the same as that produced by radiation) is a major contributor to aging, to dege

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::fce0d07296e7e122a050758868b67ef7
https://doi.org/10.1007/978-1-4615-1877-8_21

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Oxidants and mitogenesis as causes of mutation and cancer: the influence of diet

Autor: M K, Shigenaga, B N, Ames

Publikováno v: Basic life sciences. 61

A very high level oxidative damage to DNA occurs during normal metabolism. In each rat cell, the steady-state level of this damage is estimated to be about 10(6) oxidative adducts, and about 10(5) new adducts are formed daily. This endogenous DNA dam

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::8d40162f9b426ee357c483ebb6682ca4
https://pubmed.ncbi.nlm.nih.gov/8304950

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The role of cytochromes P-450 and flavin-containing monooxygenase in the metabolism of (S)-nicotine by rabbit lung

Autor: D E, Williams, M K, Shigenaga, N, Castagnoli

Publikováno v: Drug metabolism and disposition: the biological fate of chemicals. 18(4)

Rabbit lung microsomes metabolize (S)-nicotine primarily to (S)-nicotine delta 1',5'-iminium ion, which is the precursor of (S)-cotinine, the major urinary metabolite of (S)-nicotine in mammals. (S)-Nicotine-N'-oxide and normicotine are also produced

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::1b324681f23ee5bed58c2d2224909bc2
https://pubmed.ncbi.nlm.nih.gov/1976062

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In vivo oxidative DNA damage: measurement of 8-hydroxy-2'-deoxyguanosine in DNA and urine by high-performance liquid chromatography with electrochemical detection

Autor: M K, Shigenaga, J W, Park, K C, Cundy, C J, Gimeno, B N, Ames

Publikováno v: Methods in enzymology. 186

HPLC with electrochemical detection is a highly sensitive and selective method for detecting the oxidatively modified DNA residue oh8dG. By this method, the detection of oh8dG from DNA and urine offers a powerful approach for assessing in vivo oxidat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::0108de5e8f09c4ed79a8cae499605206
https://pubmed.ncbi.nlm.nih.gov/2233317

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514 OXIDANT STRESS IN HUMANSDURING CONSECUTIVE DAYS OF EXERCISE

Autor: M. K. Shigenaga, B. Frei, C. A. Viguie, Lester Packer, George A. Brooks, Bruce N. Ames

Publikováno v: Medicine & Science in Sports & Exercise. 22:S86

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0da43a3ef7f2bf0c415de012013adf0f
https://doi.org/10.1249/00005768-199004000-00514

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Metabolism-dependent covalent binding of (S)-[5-3H]nicotine to liver and lung microsomal macromolecules

Autor: M K, Shigenaga, A J, Trevor, N, Castagnoli

Publikováno v: Drug metabolism and disposition: the biological fate of chemicals. 16(3)

Incubation of (S)-[5-3H]nicotine with rabbit liver microsomes in the presence of dioxygen and NADPH results in the formation of metabolites that bind covalently to microsomal macromolecules (250-550 pmol/mg of protein/hr). The partition ratio [(S)-ni

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::e0962e74bd981bd0af48e83a9b90edce
https://pubmed.ncbi.nlm.nih.gov/2900731

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