Zobrazeno 1 - 4
of 4
pro vyhledávání: '"M. Ines Burgos"'
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. :140920
Publikováno v:
Biochemical and Biophysical Research Communications. 508:270-274
The effect on protein conformation and thermal stability was studied for β-Galactosidase (β-Gal) encapsulated in the nanopores of a silicate matrix (Eβ-Gal). Circular dichroism spectra showed that, compared with the enzyme in buffer (Sβ-Gal), Eβ
Here we studied the effect of molecular crowding on the hydrolysis of ortho- and para-nitrophenyl-β-D-galactopyranosides (ONPG, PNPG) catalysed by Escherichia coli β-Galactosidase in the presence of 0–35%w/v 6kD polyethyleneglycol (PEG6000). The
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::21aee565757c5c1ea38353cde30c3567
https://www.sciencedirect.com/science/article/abs/pii/S0006291X1930957X
https://www.sciencedirect.com/science/article/abs/pii/S0006291X1930957X
Publikováno v:
Scientific Reports
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
In the present work we studied the catalytic activity of E. coli β-Gal confined in a nanoporous silicate matrix (Eβ-Gal) at different times after the beginning of the sol-gel polymerization process. Enzyme kinetic experiments with two substrates (O