Zobrazeno 1 - 10
of 45
pro vyhledávání: '"M. Filigarová"'
Publikováno v:
Plant Protection Science, Vol 38, Iss SI 2 - 6th Conf EFPP, Pp 648-651 (2002)
In the framework of PVS eradication from breeding materials of Czech potato cultivars, the systematic research was devoted to: susceptibility of cultivars, occurrence of PVS in imported and domestic materials, and to maintenance of virus-free basic g
Externí odkaz:
https://doaj.org/article/637d3168c52e45a285cd050dc8cfab25
Publikováno v:
Plant Protection Science, Vol 38, Iss SI 2 - 6th Conf EFPP, Pp 275-277 (2002)
We determined partial nucleotide sequences of several different field isolates of PMTV with well-characterized biological properties from the Czech Republic and Denmark. Subsequently we were trying to elucidate the correlation between nucleotide sequ
Externí odkaz:
https://doaj.org/article/6a219e5cdba14754be87b7a1c0456730
Publikováno v:
Plant Protection Science. 38:648-651
Publikováno v:
Potato Research. 37:135-141
A panel of mouse monoclonal antibodies (MAbs) was prepared against potato virus A (PVA) and their reactivity was tested in various types of ELISA. The type of ELISA as well as the methods of MAbs purification played an essential role. All monoclonal
Publikováno v:
Plant Protection Science. 38:275-277
Autor:
M. Filigarová, Noemi Čeřovská
Publikováno v:
Biologia plantarum. 35:103-105
We followed the effect of storage conditions on the serological activity of the potato virus A (PVA) in the ELISA test. Storage of the purified PVA in 0.05 M borate buffer, pH 8.3 at −70°C represents the optimum conditions. The activity decreased
Publikováno v:
Acta virologica. 45(1)
Sequences of the first 300 nucleotides of coat protein (CP) genes of 7 isolates of NTN strain of potato virus Y (PVY, PVY(NTN)) were determined and compared with analogous published sequences of various isolates and strains of PVY. The sequence ident
Publikováno v:
Acta virologica. 43(6)
Eight isolates of potato virus Y NTN strain (PVY-NTN) of different origin were studied by means of monoclonal antibodies (MAbs) in non-competitive and competitive enzyme-linked immunosorbent assay (ELISA), and by immunoblot analysis of the viral coat
Publikováno v:
Acta virologica. 41(1)
Potato virus Y strain NN (PVYNN) was purified from mechanically infected plants Nicotiana tabaccum cv. Samsun by extraction of the plants with various buffers, clarification of the suspensions with chloroform or Triton X-100, high speed centrifugatio
Publikováno v:
Acta virologica. 40(1)
Four mouse monoclonal antibodies (MoAbs)) against potato virus S Andean strain (PVSA) were tested. While MoAbs 2 and 3 reacted only with complete virions and were apparently specific for epitopes dependent on quaternary structure, MoAbs 1 and 4 appar