Zobrazeno 1 - 10
of 23
pro vyhledávání: '"M. D. Yudkin"'
Publikováno v:
Europe PubMed Central
The spo-87 mutation is one of two sporulation mutations originally used to define the spo0J locus of Bacillus subtilis. We now show that it blocks sporulation after completion of prespore engulfment (stage III). Surprisingly, the operon is expressed
Publikováno v:
Journal of molecular biology. 314(3)
SpoIIAA is a key component in the network of interactions that regulate the first sporulation-specific transcription factor, sigma(F), in Bacillus subtilis. In its unphosphorylated form SpoIIAA is either phosphorylated by or forms a non-covalent comp
Publikováno v:
Molecular microbiology. 40(1)
Sigma-F, the first sporulation-specific transcription factor of Bacillus subtilis, is regulated by an anti-sigma factor SpoIIAB, which can also act as a protein kinase that phosphorylates the anti-anti-sigma factor SpoIIAA. The time course of phospho
Publikováno v:
Microbiology. 135:767-775
SUMMARY: The spoIIA locus of Bacillus licheniformis was cloned into the phage vectorφ105J9; selection was based on the ability of the clone to complement mutations in both the first and the last of the spoIIA genes of B. subtilis. The B. licheniform
Unstable mutations that relieve catabolite repression of tryptophanase synthesis by Escherichia coli
Autor:
M D Yudkin
Publikováno v:
Journal of Bacteriology. 130:57-61
From strains of Escherichia coli that carry deletions of the trp region, five different mutants were isolated that were capable of synthesizing tryptophanase at unusually high rates in conditions of severe catabolite repression. Notwithstanding the c
Autor:
M. K. White, M. D. Yudkin
Publikováno v:
Journal of General Microbiology. 114:471-475
Summary: Nine independent mutants deficient in tryptophanase activity were isolated. Each mutation was transferred to a specialized transducing phage that carries the tryptophanase region of the Escherichia coli chromosome. The nine phages thus produ
Publikováno v:
Molecular and General Genetics MGG. 187:244-247
By labelling wild-type Bacillus subtilis for 5 min with [35S]-methionine either at the time of resuspension in starvation medium or 1, 2 or 3 h later, and subjecting cell extracts to two-dimensional gel electrophoresis, Yudkin et al. (J. Gen. Microbi
Publikováno v:
Microbiology. 128:2165-2177
Summary: Cultures were labelled with l-[35S]methionine for 5 min periods immediately after resuspension in sporulation medium (t 0) or at hourly intervals thereafter (t 1, t 2 or t 3). Cells were harvested and lysed very rapidly at low temperature in
Autor:
M. D. Yudkin, D. M. Mascarenhas
Publikováno v:
Microbiology. 118:405-410
The specialized transducing phage lambda cysB (Borck et al., 1976) was found to carry about 5 kilobases of Escherichia coli DNA. It was shown to have an intact cysB gene but none of the known neighbouring genetic loci. The phage (which is known to be
Autor:
M. D. Yudkin
Publikováno v:
Microbiology. 133:475-481
SUMMARY: The spoIIAC gene was cloned from chromosomal DNA of seven spoIIAC mutants of Bacillus subtilis, and the complete sequence of the gene was determined for each mutant. Three of the mutants proved to have chain-terminating mutations (one of whi