Výsledky vyhledávání - "M. C. G. Monaco"

A fluorescent in situ hybridization method in flow cytometry to detect HIV-1 specific RNA

Autor: Annalisa Facchini, Spartaco Santi, M. C. G. Monaco, Gina Lisignoli, A. Degrassi, A. Piacentini, Rosa Maria Borzì, Luca Cattini

Publikováno v: Journal of Immunological Methods. 193:167-176

In HIV+ patients, the presence of HIV-RNA in plasma and circulating cells has been reported to be a marker of progression but the percentage of transcriptionally active infected cells remains unclear. We have developed a reliable fluorescent in situ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::75bb45b5233064adb54abe456264249d
https://doi.org/10.1016/0022-1759(96)00070-1

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In vitro cultured stromal cells from human tonsils display a distinct phenotype and induce B cell adhesion and proliferation

Autor: S. Lavaroni, M. C. G. Monaco, Ornella Belvedere, Andrea Facchini, David M. Hilbert, Gina Lisignoli, A. Degrassi, S. Toneguzzi, Luca Cattini

Publikováno v: European Journal of Immunology. 26:17-27

Peripheral lymphoid tissues contain a fibroblastic cell type referred to as stromal cells or reticulum cells which interact with lymphocytes as part of the lymphoid microenvironment. After isolation from human tonsils and expansion in vitro we analyz

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc0229073a760c98636a0f7520efcce8
https://doi.org/10.1002/eji.1830260104

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Distribution and lytic activity of NK cell subsets in the elderly

Autor: Erminia Mariani, M. C. G. Monaco, Annalisa Facchini, Luca Cattini, M. Sinoppi

Publikováno v: Mechanisms of Ageing and Development. 76:177-187

Old subjects present an increased number of NK cells associated with a decreased lytic activity of isolated and cloned CD16 cells. Recently, two new surface molecules of 58 kDa, identified by the monoclonal antibodies GL183 and EB6, have been describ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57cfc58f41c0a5796b18cac29f1d4824
https://doi.org/10.1016/0047-6374(94)91592-x

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Standardization of a micro-cytotoxicity assay for human natural killer cell lytic activity

Autor: Erminia Mariani, Annalisa Facchini, M. C. G. Monaco, A. R. Mariani, S. Sgobbi, J.F. de Zwart

Publikováno v: Journal of Immunological Methods. 172:173-178

Cytotoxicity assays are widely used to evaluate the functional activity of NK and T cells against tumour target cells and the release of radioactive sodium chromate from labelled target cells is still the most commonly used marker of target lysis in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d60e2b6bf33ac2366b21f9f4b48a35d0
https://doi.org/10.1016/0022-1759(94)90104-x

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In vitro Toxicity of 2′,3′-Dideoxy-3′-Thiacytidine (BCH189/3TC), a New Synthetic Anti-HIV-1 Nucleoside

Autor: Erminia Mariani, A. Degrassi, Annalisa Facchini, Luca Cattini, Gina Lisignoli, M. C. G. Monaco

Publikováno v: Antiviral Chemistry and Chemotherapy. 3:299-303

2′,3′-Dideoxy-3′-thiacytidine (BCH189 or 3TC, IAF BioChem International Inc., Montreal, Canada) is a new synthetic anti-HIV-1 dideoxynucleoside with a sulphur atom in the heterocycle ring. Preclinical studies showed that this compound at 5–10

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f9c9b03af8584e7dda7360c544e34c13
https://doi.org/10.1177/095632029200300508

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Different pattern of cytokine production and mRNA expression by lymphoid and non-lymphoid cells isolated from human palatine tonsil

Autor: M. C. G. Monaco, Annalisa Facchini, S. Toneguzzi, M. Tomassetti, C. Pozzi, Gina Lisignoli

Publikováno v: International journal of clinicallaboratory research. 28(1)

To investigate the cytokines involved in the interaction between circulating (B and T lymphocytes) and non-circulating (stromal cells) elements present in lymphoid tissue, highly purified populations were isolated from human tonsils and the cytokine

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e34eb3338cc270c6927ba65dd973b297
https://pubmed.ncbi.nlm.nih.gov/9594359

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In vitro immunotoxicity of +/- 2'-deoxy-3'-thiacytidine, a new anti-HIV agent

Autor: A. Facchini, S. Toneguzzi, Gina Lisignoli, M. C. G. Monaco, E. Ricchi, A. Degrassi, P. Costigliola

Publikováno v: Scopus-Elsevier

SUMMARYThe present study compares the in vitro effect of (±)-2′-deoxy-3′-lhiacylidine (BCH 189) a new synthetic anti-HIV-l dideoxynucleosidc. with 3′-azido-3′-deoxythymidinc (AZT) on the immune function of lymphocytes from 10 normal and 12 H

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18941621ceb69c5a7ec9dc4b014bd822
https://europepmc.org/articles/PMC1554772/

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Transfer of HIV-1 to human tonsillar stromal cells following cocultivation with infected lymphocytes

Autor: Lina Cirillo, Nicoletta Zini, David M. Hilberth, Patrizia Sabatelli, A. Degrassi, F. Saverio Ambesi-Impiombato, Gina Lisignoli, Andrea De Maria, Nadir M. Maraldi, M. C. G. Monaco, Andrea Facchini

Publikováno v: Europe PubMed Central

The susceptibility of normal human tonsillar stromal cells (HTSCs) to infection by HIV-1 was assessed using transmission electron microscopy (TEM), immunocytochemistry, and HIV-1-specific PCR analyses. Our results demonstrate that HTSCs are efficient

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b4ef2751d253206f1db68a7a70d4a19
http://europepmc.org/abstract/med/8074931

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