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Short DNA Fragments without Sequence Similarity Are Initiation Sites for Replication in the Chromosome of the YeastYarrowia lipolytica

Autor: M. Chasles, Philippe Pasero, Andrée Lepingle, Claude Gaillardin, Laurence Vernis, Philippe Fournier

Publikováno v: Molecular Biology of the Cell
Molecular Biology of the Cell, American Society for Cell Biology, 1999, 10, pp.757-769

We have previously shown that both a centromere (CEN) and a replication origin are necessary for plasmid maintenance in the yeastYarrowia lipolytica ( Vernis et al., 1997 ). Because of this requirement, only a small number of centromere-proximal repl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::faf3b9ad50dc7930bd283b9eebb13680
https://doi.org/10.1091/mbc.10.3.757

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Colocalization of centromeric and replicative functions on autonomously replicating sequences isolated from the yeast Yarrowia lipolytica

Autor: D M Ogrydziak, Philippe Fournier, C Feynerol, A M Ribet, D Yaver, B Kudla, M. Chasles, A Peito, A Abbas, Jian-Wu Xuan

Publikováno v: Proceedings of the National Academy of Sciences. 90:4912-4916

Two sequences (ARS18 and ARS68) displaying autonomous replication activity were previously cloned in the yeast Yarrowia lipolytica. The smallest fragment (1-1.3 kb) required for extrachromosomal replication of a plasmid is significantly larger in Y.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9e29734f1febeee12cbb809579606b7a
https://doi.org/10.1073/pnas.90.11.4912

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Use of ars18 based vectors to increase protein production in Yarrowia lipolytica

Autor: Claude Gaillardin, C. La Bonnardière, Jean-Marc Nicaud, M. Chasles, P. Fournier

Publikováno v: Journal of Biotechnology
Journal of Biotechnology, Elsevier, 1991, 19, pp.259-270

The isolation of ars sequence from the yeast Yarrowia lipolytica has recently been reported (Fournier et al., 1991). Vectors containing ars18 have been used to increase homologous and heterologous protein production. Examples presented are the Yarrow

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98fb33381ba3bfb530366029eb6f2964
https://doi.org/10.1016/0168-1656(91)90063-2

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Scarcity ofars sequences isolated in a morphogenesis mutant of the yeastYarrowia lipolytica

Autor: Claude Gaillardin, Ph. Fournier, L. Guyaneux, M. Chasles

Publikováno v: Yeast. 7:25-36

Previous attempts is isolate autonomously replicating sequences (ars) from the dimorphic yeast Yarrowia lipolytica have been unsuccessful. We isolated a Fil- mutant unable to produce hyphae and growing only in a yeast form to facilitate ars isolation

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::07c5be89cf0d94b03dac6e7800f3e88f
https://doi.org/10.1002/yea.320070104

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Two Hrd1p homologues in the yeast Yarrowia lipolytica which act in different pathways

Autor: Jean-Marie Beckerich, A. Babour, Anita Boisramé, M. Chasles

Publikováno v: Molecular Genetics and Genomics
Molecular Genetics and Genomics, Springer Verlag, 2006, 275, pp.242-250
Molecular Genetics and Genomics, Springer Verlag, 2006, 275 (3), pp.242-250. ⟨10.1007/s00438-005-0084-6⟩

International audience; The endoplasmic reticulum associated degradation (ERAD) is a process widespread in eukaryotes that enable cells to get rid of unfolded or unassembled polypeptides which jam the endoplasmic reticulum compartment. In order to im

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ccd31b67060a791752880660ab3fdbf8
https://hal.archives-ouvertes.fr/hal-00088726

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Only centromeres can supply the partition system required for ARS function in the yeast Yarrowia lipolytica

Autor: Kohji Uchida, Leonora Poljak, Philippe Fournier, Laurence Vernis, Emmanuel Käs, Masayoshi Matsuoka, M. Chasles, Serge Casaregola, Claude Gaillardin

Publikováno v: Journal of Molecular Biology
Journal of Molecular Biology, Elsevier, 2001, 305, pp.203-217

Autonomously replicating sequences (ARSs) in the yeast Yarrowia lipolytica require two components: an origin of replication (ORI) and centromere (CEN) DNA, both of which are necessary for extrachromosomal maintenance. To investigate this cooperation

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3a0482c21c1bad5022877482de4d0cd
https://hal.inrae.fr/hal-02676034

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An origin of replication and a centromere are both needed to establish a replicative plasmid in the yeast Yarrowia lipolytica

Autor: Laurence Vernis, J A Huberman, M. Chasles, A Abbas, Christine Brun, Claude Gaillardin, Philippe Fournier

Publikováno v: Molecular and Cellular Biology
Molecular and Cellular Biology, American Society for Microbiology, 1997, 17 (4), pp.1995-2004
Molecular and Cellular Biology, 1997, 17 (4), pp.1995-2004

Two DNA fragments displaying ARS activity on plasmids in the yeast Yarrowia lipolytica have previously been cloned and shown to harbor centromeric sequences (P. Fournier, A. Abbas, M. Chasles, B. Kudla, D. M. Ogrydziak, D. Yaver, J.-W. Xuan, A. Peito

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f75e0aedbd4d510912b768b67ac37913
https://hal-amu.archives-ouvertes.fr/hal-01596228/file/1997_MCB.pdf

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