Zobrazeno 1 - 10
of 15
pro vyhledávání: '"M V, Serebriakova"'
Publikováno v:
Applied Biochemistry and Microbiology. 51:818-826
The recombinant E7 oncoprotein gene of type 16 human papilloma virus (conjugated with the heat shock protein 70 (E7(16)-HSP70)) was expressed in Saccharomyces cerevisiae yeast cells. A number of chromatographic isolations were conducted to obtain the
Publikováno v:
Biotekhnologiya. :29-37
A gene for a recombinant E7 oncoprotein of human papillomavirus type 16 conjugated with a heat shock protein 70 (E7(16)-HSP70) has been expressed in Saccharomyces cerevisiae. A number of chromatographic separations was performed in order to primarily
Autor:
F M, Eroshkin, A V, Baĭramov, O V, Aver'ianova, E A, Solov'eva, M V, Serebriakova, A G, Zaraiskiĭ, N Iu, Martynova
Publikováno v:
Bioorganicheskaia khimiia. 39(2)
We have developed methods for producing recombinant proteins of Noggin family (Noggin1 and Noggin2 of the Xenopus laevis frog) that can interact with BMP factors of TGF-beta superfamily. The genetic constructs which allow one to effectively obtain No
Publikováno v:
Bioorganicheskaia khimiia. 37(6)
The method of direct introduction 18O isotopes in peptides and proteins carboxylic groups through the exchange with H218O in the presence of TFA is shown. The isotope label is steady enough in awide range of pH. Because the labeled compounds retain t
Autor:
A V, Zakharov, I V, Smirnov, M V, Serebriakova, M A, Dronina, A V, Kaznacheeva, I N, Kurkova, A A, Belogurov, A, Friboulet, N A, Ponomarenko, A G, Gabibov, T V, Bobik
Publikováno v:
Molekuliarnaia biologiia. 45(1)
Expression of recombinant antibodies in mammalian cells is one of key problems in immunobiotechnology. Alternatively, expression of a broad panel of antibodies and of their fragments may be effectively done in yeast cells. We obtained expression stra
Autor:
P V, Sergiev, I A, Osterman, I V, Prokhorova, M V, Nesterchuk, O V, Sergeeva, A Ia, Golovina, I A, Demina, M A, Galiamina, M V, Serebriakova, O A, Dontsova
Publikováno v:
Bioorganicheskaia khimiia. 37(1)
In this work we describe methodology for studying the role of bacterial ribosome modification in the regulation of gene expression. Ribosomal components modification influences translation efficiencies of certain mRNAs. Proteome analysis allows us to
Autor:
V V, Radchenko, E V, Il'nitskaia, V E, Tret'iakov, M V, Serebriakova, Z I, Storozheva, T M, Shuvaeva, V M, Lipkin
Publikováno v:
Bioorganicheskaia khimiia. 36(5)
Novel protein with a molecular mass of ~43 kDa from rat olfactory epithelium in pathophysiological conditions was discovered. Its amino acid sequence and affiliation with the family 18 glycohydrolase subgroup of chitinase-like proteins YM-1 were dete
Autor:
O N, Tatarinova, N V, Gorokhovets, V A, Makarov, G A, Posypanova, M V, Serebriakova, G E, Pozmogova
Publikováno v:
Vestnik Rossiiskoi akademii meditsinskikh nauk. (1)
A human alpha-fetoprotein fragment (AFP) modified with oligocationic homologs of nuclear localization signal was used to construct new target cell-selective DNA-carrier proteins. The new recombinant vectors containing C- or N-terminal polynucleotide-
Publikováno v:
Biomeditsinskaia khimiia. 53(6)
In the human myocardium tissue, proteomic technologies revealed the products of 2 genes (alpha-actin and albumin), existing as fragments; their appearance and increased contents correlated with age. The age-related variants differ from the mature for
Autor:
I I, Tiuriaeva, O A, Mirgorodskaia, O A, Cherepanova, E P, Podol'skaia, M V, Serebriakova, V A, Ivanov
Publikováno v:
Tsitologiia. 47(12)
The laminin affinity chromatography was used for isolating laminin-binding proteins from the plasma membrane of Zajdela hepatoma cells synthesizing laminin. These were components with mol. weights about 80, 67, 60, 55, 52, 48 and 43 kDa. The isolatio