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pro vyhledávání: '"M V, Honchar"'
Publikováno v:
Ukrains'kyi biokhimichnyi zhurnal (1999 ). 81(6)
Enzymatic method for formaldehyde assay, based on formaldehyde dehydrogenase from the recombinant strain of the yeast Hansenula polymorpha, was developed. Linear detection range for the proposed method was estimated to be from 0.01 to 0.05 mM, and se
Publikováno v:
Ukrains'kyi biokhimichnyi zhurnal (1999 ). 74(2)
A new oxidase-coupled colorimetric method for analysis of L-lactate in biological fluids has been developed without use of peroxidase. The method is based on lactate oxidase-catalysed transformation of lactate to pyruvate which is determined photomet
Publikováno v:
Ukrains'kyi biokhimichnyi zhurnal (1999 ). 72(4-5)
An extended definition of the term "metabolic engineering" is given and main spheres of its using in fundamental studies and modern biotechnology are discussed in this article. Emphasis is made on specific using the approaches of metabolic engineerin
Autor:
M V, Honchar
Publikováno v:
Ukrainskii biokhimicheskii zhurnal (1. 70(5)
The sensitive method for peroxidative assays of hydrogen peroxide (up to 5 nmole in 1 ml) and oxidases' substrates (glucose, ethanol) has been developed by use of a non-cancerogenic chromogen--3,3', 5,5'-tetramethylbenzidine (TMB), horseradish peroxi
Publikováno v:
Ukrainskii biokhimicheskii zhurnal (1. 63(6)
The photometric method is suggested for ethanol analysis by means of purified alcohol oxidase isolated from a wild strain of methylotrophic yeast Hansenula polymorpha. Three different chromogenic systems for peroxidative oxidation together with alcoh