Delays during PBMC isolation have a moderate effect on yield, but severly compromise cell viability

Autor: T Golke, P Mucher, P Schmidt, A Radakovics, M Repl, P Hofer, T Perkmann, M Fondi, KG Schmetterer, H Haslacher

Objectives Peripheral blood mononuclear cells (PBMCs) are a versatile material for clinical routine as well as for research projects. However, their isolation via density gradient centrifugation is still time-consuming. When samples are taken beyond

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba6704297a14469866f1ae1057256af0
https://doi.org/10.1101/2022.01.02.22268625

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Preanalytical stability of SARS-CoV-2 anti-nucleocapsid antibodies.

Autor: Niedrist T; Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz, Graz, Austria., Kriegl L; Department of Internal Medicine, Division of Infectiology, Medical University Graz, Graz, Austria., Zurl CJ; Department of Paediatrics and Adolescent Medicine, Division of General Paediatrics, Medical University of Graz, Graz, Austria., Schmidt F; Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria., Perkmann-Nagele N; Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria., Mucher P; Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria., Repl M; Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria., Flieder I; Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria., Radakovics A; Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria., Sieghart D; Department of Medicine III, Division of Rheumatology, Medical University of Vienna, Vienna, Austria., Radner H; Department of Medicine III, Division of Rheumatology, Medical University of Vienna, Vienna, Austria., Aletaha D; Department of Medicine III, Division of Rheumatology, Medical University of Vienna, Vienna, Austria., Binder CJ; Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria., Gülly C; Center for Medical Research (ZMF), Medical University of Graz, Graz, Austria., Krause R; Department of Internal Medicine, Division of Infectiology, Medical University Graz, Graz, Austria., Herrmann M; Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz, Graz, Austria., Wagner OF; Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria., Perkmann T; Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria., Haslacher H; Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria.

Publikováno v: Clinical chemistry and laboratory medicine [Clin Chem Lab Med] 2022 Nov 04; Vol. 61 (2), pp. 332-338. Date of Electronic Publication: 2022 Nov 04 (Print Publication: 2023).