Zobrazeno 1 - 10
of 431
pro vyhledávání: '"M R, Kula"'
Publikováno v:
Biotechnology and Bioengineering. 104:217-239
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b78b0a7971a175475febb9f582145b9f
https://doi.org/10.1002/bit.22492
https://doi.org/10.1002/bit.22492
Autor:
M.-R. Kula, Jürgen Hubbuch
Publikováno v:
Bioprocess and Biosystems Engineering. 31:241-259
In recent years, confocal laser scanning microscopy has been developed into a non-invasive tool to probe intra-particle profiles of protein in chromatographic adsorbents. A necessary prerequisite when using this technique lies in the labeling of prot
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19671f465abf5433fc3835c4ade6d658
https://doi.org/10.1007/s00449-008-0197-5
https://doi.org/10.1007/s00449-008-0197-5
Autor:
Dirk Weuster-Botz, S. Dilsen, D. Tippe, Markus Halfar, A. Sandgathe, Jörg Thömmes, Roland Freudl, M.-R. Kula, Jochen Meens
Publikováno v:
Process Biochemistry. 38:1351-1363
Recombinant human calcitonin (hCT) can be expressed by Staphylococcus carnosus as a precursor peptide being part of a secreted fusion protein which contains the propeptide of a Staphylococcus hyicus lipase as secretion carrier and multiple repeats of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::07df98ebd72c4ab03c701797843f70b8
https://doi.org/10.1016/s0032-9592(02)00332-1
https://doi.org/10.1016/s0032-9592(02)00332-1
Autor:
M.-R. Kula, S. Neumann
Publikováno v:
Applied Microbiology and Biotechnology. 58:772-780
The peptide amidase (Pam) from the gram-negative bacterium Stenotrophomonas maltophilia catalyzes predominantly the hydrolysis of the C-terminal amide bond in peptide amides. Its gene ( pam) was isolated by Southern hybridization using a DNA probe de
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::426bf993a567407a37b037f86d3d62bd
https://doi.org/10.1007/s00253-002-0943-6
https://doi.org/10.1007/s00253-002-0943-6
Autor:
M. R. Kula, L. M. Ducrey Santopietro
Publikováno v:
Folia Microbiologica. 46:277-288
Extrachromosomal elements were found in a strain of X. dendrorhous, and were characterized as linear DNA forming two well defined groups, pPh1 with 3 high-copy-number molecules, pPh11 (6.9 kb), pPh12 (5.7), pPh13 (4.7), and pPh2 with 2 low-copy-numbe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3770e415e01c8342bbe4e1563dfb5551
https://doi.org/10.1007/bf02815614
https://doi.org/10.1007/bf02815614
Publikováno v:
Applied Microbiology and Biotechnology. 55:354-361
Thirty-one different actinomycete strains were used in a genetic screening using PCR and Southern hybridization methods to detect N-acetylamino acid racemases (AAR) in order to obtain enzymes with different properties. Cloning and sequencing of a 2.5
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a7af820d4932f547cae46445d29efee2
https://doi.org/10.1007/s002530000508
https://doi.org/10.1007/s002530000508
Publikováno v:
Biocatalysis and Biotransformation. 19:51-68
During the enzymatic synthesis of open chain ketose-1-phosphates on a preparative scale using fructose-1,6-bisphosphate aldolases from Staphylococcus carnosus a successive reaction of excess aldehyde with the primary reaction product was observed lea
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f820191e8aeddbabe51ea5eed516a960
https://doi.org/10.3109/10242420109103516
https://doi.org/10.3109/10242420109103516
Publikováno v:
Bioseparation. 10:229-236
The parameters important for an optimisation of cloud point extraction in technical scale were investigated using a genetically engineered fusion protein derived from endoglucanase I expressed in Trichoderma reesei and the nonionic polyoxyethylene Ag
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::dcfe7faec304bb0dfdab3a24ba83ac47
https://doi.org/10.1023/a:1016379111336
https://doi.org/10.1023/a:1016379111336
Publikováno v:
Journal of Chromatography A. 873:195-208
Expanded bed adsorption (EBA) is a primary recovery operation allowing the adsorption of proteins directly from unclarified feedstock, e.g. culture suspensions, homogenates or crude extracts. Thus solid-liquid separation is combined with adsorptive p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::482499ecbff1add6559983f719d5152d
https://doi.org/10.1016/s0021-9673(99)01308-4
https://doi.org/10.1016/s0021-9673(99)01308-4