Zobrazeno 1 - 10
of 51
pro vyhledávání: '"M N Margolies"'
Autor:
B Parhami-Seren, M N Margolies
Publikováno v:
The Journal of Immunology. 157:2066-2072
We showed previously that heavy chain gene junctional amino acid differences among unmutated p-azophenylarsonate (Ars) Abs that share a unique gene segment combination encoding these V regions, termed "canonical," alter affinity. To determine the con
Publikováno v:
The Journal of Immunology. 154:3351-3358
The crystal model of the complex of the somatically mutated anti-p-azophenylarsonate (Ars) Ab 36-71 F(ab) with phenylarsonate reveals that six residues (Asn35, Trp47, Tyr50, Ser99, and Tyr106 in the H chain and Arg96 in the L chain) contact hapten. F
Publikováno v:
The Journal of Immunology. 152:146-152
During the acquisition of humoral immunity, the process of somatic hypermutation introduces nucleotide substitutions into expressed antibody (Ab) V region genes. Studies employing in vitro mutagenesis have shown that recurrent mutations observed in v
Publikováno v:
The Journal of Immunology. 150:1829-1837
Murine A/J anti-p-azophenylarsonate mAb that express a dominant cross-reactive Id are encoded by a single set of germ-line VH and VL region genes. The crystal structure of the Fab of antibody 36-71, which uses this canonical set of genes but is somat
Publikováno v:
The Journal of Immunology. 145:2718-2724
A set of high affinity antidigoxin antibodies were previously identified with high homologous V kappa 1A L chain sequences but were associated with two entirely different VH regions and two dramatically different specificities for digoxin analogs. An
Publikováno v:
The Journal of Immunology. 145:2340-2346
A cluster of four consecutive CDR2 somatic mutations are shared by the VH regions of two independently isolated hybridoma antibodies with specificity for p-azophenylarsonate (Ars). The mutations appear to be derived by a series of independent events.
Publikováno v:
Journal of molecular biology. 301(1)
Since the initial report of the development of methodology to generate high-affinity digitalis-specific (digoxin) antibodies, these antibodies have proven extremely useful tools to monitor digoxin levels in digitalized patients and, as Fab fragments,
Monoclonal antibodies that distinguish between two related digitalis glycosides, ouabain and digoxin
Publikováno v:
Journal of immunology (Baltimore, Md. : 1950). 163(8)
The exogenous digitalis glycosides, ouabain and digoxin, have been widely used in humans to treat congestive heart failure and cardiac arrhythmias. Several reports have also pointed to the existence of endogenous ouabain- and digoxin-like compounds,
Publikováno v:
Journal of immunology (Baltimore, Md. : 1950). 160(12)
We previously showed that a single mutation at heavy (H) position 35 of Abs specific for p-azophenylarsonate (Ars) resulted in acquisition of binding to the structurally related hapten p-azophenylsulfonate (Sulf). To explore the sequence and structur
Autor:
B, Parhami-Seren, M N, Margolies
Publikováno v:
Journal of immunology (Baltimore, Md. : 1950). 157(5)
We showed previously that heavy chain gene junctional amino acid differences among unmutated p-azophenylarsonate (Ars) Abs that share a unique gene segment combination encoding these V regions, termed "canonical," alter affinity. To determine the con