Zobrazeno 1 - 10
of 12
pro vyhledávání: '"M M, Gusiatiner"'
Publikováno v:
Mikrobiologiia. 67(3)
The efficiency of the electrotransformation of intact cells of corynebacteria by a solitary impulse with a complex shape amounted to 10(6) transformants/microgram of plasmid pNV1 DNA at an electric field strength of 14.2 kW/cm; the voltage-current cu
Autor:
N O, Bukanov, O O, Nashchokina, S A, Borinskaia, A V, Lobashev, M Iu, Fonshteĭn, M M, Gusiatiner, V G, Debabov, N K, Iankovskiĭ
Publikováno v:
Genetika. 34(3)
A representative genomic library of the Corynebacterium glutamicum ATCC 13032 genes in a cosmid vector Lorist6 was created. The cosmids contain inserts of bacterial DNA obtained by partial digestion with the Sau3A I restrictase. Five hundred and thir
Autor:
M Iu, Peredel'chuk, N O, Bukanov, Iu V, Smirnov, Iu V, Rostova, N D, Fedorova, A L, Okorokov, M M, Gusiatiner, N K, Iankovskiĭ
Publikováno v:
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (5-6)
Plasmids carrying an asd gene from a mutant. S-(2-aminoaethyl)-L-cysteine resistant strain of Corynebacterium glutamicum were selected from a clonoteque constructed on a plasmid cloning vector pSL5 by complementation of asd mutation in Escherichia co
Autor:
O E, Tolmachev, M M, Gusiatiner
Publikováno v:
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (1-2)
Autor:
O E, Tolmachev, M M, Gusiatiner
Publikováno v:
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (11)
Construction of the shuttle cloning vectors for Escherichia coli-Brevibacterium flavum system is described. Expression of the Sp/Sm resistance determinant derived from the Corynebacterium plasmid pCG4 was registered in Escherichia coli cells. The gen
Publikováno v:
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (10)
Properties of a virulent Brevibacterium flavum bacteriophage phi BSh6 were studied. The phage was placed in morphological group B1 according to Ackerman classification, head diameter being 74-3 nm, tail length being 337 +/- 15 nm. The phage was shown
Publikováno v:
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (2)
The functioning of Escherichia coli threonine operon isolated genes thrB and thrC was studied by using the genetic complementation and enzymatic activity determination techniques. A new gene thrBC was obtained by the genes merging. The genes thrB and
Autor:
O Iu, Beskrovnaia, N O, Bukanov, M Iu, Fonshteĭn, M M, Gusiatiner, A L, Okorokov, O O, Nashchokina, N K, Iankovskiĭ
Publikováno v:
Genetika. 26(3)
A library of EcoRI DNA fragments from Brevibacterium flavum was constructed using plasmid vector. The genes complementing ThrA2 and ThrB mutations in Escherichia coli were identified in the library. The gene thrA2 of B. flavum codes for mutant enzyme
Publikováno v:
Prikladnaia biokhimiia i mikrobiologiia. 22(1)
An active transport system high specific for 1-lysine was found in the cells of the wild strain of Corynebacterium glutamicum, Km being about 10 microM. Accumulation of lysine was higher, if the cells were cultivated on a medium containing glucose. T
Publikováno v:
Genetika. 16(2)
During the derepression of threonine and isoleucine-valine operons the increase of activity of homoserine dehydrogenase and threonine deaminase respectively occurs only in relA+ strains of Escherichia coli K-12, while the activity of these enzymes in