Zobrazeno 1 - 10
of 23
pro vyhledávání: '"M L Howell"'
Publikováno v:
Journal of Biological Chemistry. 268:7155-7162
Ribonucleotide reductase has been suggested as a rate-limiting enzyme in DNA synthesis, partly because activities of the enzyme in cell-free preparations are low relative to rates needed to sustain DNA replication at observed rates. Vaccinia virus, w
Publikováno v:
Journal of Virology. 65:2290-2298
Hydroxyurea, an inhibitor of ribonucleotide reductase, blocks replication of vaccinia virus. However, when medium containing hydroxyurea and dialyzed serum was supplemented with deoxyadenosine, the block to viral reproduction was circumvented, provid
Regulation of the macrophage vacuolar ATPase and phagosome-lysosome fusion by Histoplasma capsulatum
Publikováno v:
Journal of immunology (Baltimore, Md. : 1950). 162(10)
Histoplasma capsulatum (Hc) maintains a phagosomal pH of about 6.5. This strategy allows Hc to obtain iron from transferrin, and minimize the activity of macrophage (Mo) lysosomal hydrolases. To determine the mechanism of pH regulation, we evaluated
Publikováno v:
Operative dentistry. 21(2)
The effect of microabrasion on human enamel has been well documented; however, no information is available on its effect on dentin or restorative materials. The purpose of this study was to evaluate the effect of the microabrasion technique on the su
Publikováno v:
Molecular biology of the cell. 7(1)
Protein sorting in eukaryotic cells is mainly done by specific targeting of polypeptides. The present evidence from oocytes, neurons, and some other polarized cells suggests that protein sorting can be further facilitated by concentrating mRNAs to th
Publikováno v:
Operative dentistry. 18(2)
This study evaluated in vitro the necessity of retention grooves in approximal boxes in class 2 preparations with faciolingually wide occlusal extensions (occlusal isthmus width 1.8 mm). Thirty-six class 2 mesio-occlusal cavities were prepared in sou
Publikováno v:
Operative dentistry. 17(2)
This study evaluated several means of providing retention for the approximal box in very conservative class 2 preparations (occlusal isthmus width 0.7 mm). Sixty class 2 mesio-occlusal cavities were prepared in sound human maxillary premolar teeth. F
Publikováno v:
The Journal of biological chemistry. 267(3)
During its infectious cycle, vaccinia virus expresses a virus-encoded ribonucleotide reductase which is distinct from the host cellular enzyme (Slabaugh, M.B., and Mathews, C.K. (1984) J. Virol. 52, 501-506; Slabaugh, M.B., Johnson, T.L., and Mathews
Autor:
M L, Howell, K M, Blumenthal
Publikováno v:
The Journal of biological chemistry. 266(20)
We have previously demonstrated that a synthetic gene encoding Cerebratulus lacteus neurotoxin B-IV can be expressed in bacteria, and the recombinant toxin purified and refolded (Howell, M. L., and Blumenthal, K. M. (1989) J. Biol. Chem. 264, 15268-1
Autor:
Kenneth M. Blumenthal, M L Howell
Publikováno v:
Journal of Biological Chemistry. 264:15268-15273
A synthetic gene encoding Cerebratulus lacteus neurotoxin B-IV has been designed, cloned, and expressed in Escherichia coli. Although expression of the toxin alone appears to be incompatible with host viability, large amounts could be synthesized as