Výsledky vyhledávání - "M J, Casadaban"

Analysis of the host ranges of transposon bacteriophages Mu, MuhP1, and D108 by use of lipopolysaccharide mutants of Salmonella typhimurium LT2

Autor: M J Casadaban, Cesar Roncero, K E Sanderson

Publikováno v: Journal of Bacteriology. 173:5230-5233

The lipopolysaccharide receptors for the mutator bacteriophages Mu, MuhP1, and D108 were investigated with lipopolysaccharide mutants of Salmonella typhimurium LT2. Mu adsorbed only to mutants lacking the terminal O antigen but retaining the main cha

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f3e471738b6e72dad03151d44731417
https://doi.org/10.1128/jb.173.16.5230-5233.1991

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Specificity of mini-Mu bacteriophage insertions in a small plasmid

Autor: M J Casadaban, B A Castilho

Publikováno v: Journal of Bacteriology. 173:1339-1343

Target site selection for bacteriophage Mu transposition was studied in pools of over 10(7) independent mini-Mu insertions in pUC9, selected by transduction of the plasmid. Insertions in both orientations were clustered in three regions and, within t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9ef0b095ce6b6fa61f6bab9f752d84b
https://doi.org/10.1128/jb.173.3.1339-1343.1991

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Pseudomonas aeruginosa transposable bacteriophages D3112 and B3 require pili and surface growth for adsorption

Autor: Cesar Roncero, M J Casadaban, Aldis Darzins

Publikováno v: Europe PubMed Central

Pseudomonas aeruginosa transposable bacteriophages D3112 and B3 were found to require pili for infection. Seventy mutants of P. aeruginosa PAO selected by resistance to D3112 or B3 were also resistant to the phage not used in the selection and sugges

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac2a4468760812f60b19722e4bd1d7c9
https://doi.org/10.1128/jb.172.4.1899-1904.1990

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Nucleotide sequences required for Tn3 transposition immunity

Autor: M J Casadaban, J A Kans

Publikováno v: Journal of Bacteriology. 171:1904-1914

The Tn3 transposon inserts at a reduced frequency into a plasmid already containing a copy of Tn3, a phenomenon known as transposition immunity. The cis-acting site on Tn3 responsible for immunity was mapped by deletions from each side to be within t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b689c9cfc82cd7952ccde4c57d36f277
https://doi.org/10.1128/jb.171.4.1904-1914.1989

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In vivo cloning of Pseudomonas aeruginosa genes with mini-D3112 transposable bacteriophage

Autor: M J Casadaban, Aldis Darzins

Publikováno v: Journal of Bacteriology. 171:3917-3925

The transposition properties of the Pseudomonas aeruginosa mutator bacteriophage D3112 were exploited to develop an in vivo cloning system. Mini-D replicon derivatives of D3112 were constructed by incorporating broad host range plasmid replicons betw

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17a4886fd91948b3572a47261595e991
https://doi.org/10.1128/jb.171.7.3917-3925.1989

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In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals

Autor: M J Casadaban, Joany Chou, S N Cohen

Publikováno v: Journal of Bacteriology. 143:971-980

We report the construction and use of a series of plasmid vectors suitable for the detection and cloning of translational control signals and 5' coding sequences of exogenously derived genes. In these plasmids, the first eight codons of the amino-ter

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b24bb67fb3f628c0f443332106d1446
https://doi.org/10.1128/jb.143.2.971-980.1980

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Fusion of the Saccharomyces cerevisiae leu2 gene to an Escherichia coli beta-galactosidase gene

Autor: M J Casadaban, A E Martinez-Arias

Publikováno v: Molecular and Cellular Biology. 3:580-586

The promoter and translation initiation region of the Saccharomyces cerevisiae leu2 gene was fused to the Escherichia coli beta-galactosidase gene. This fusion located the control region of the leu gene and orientated its direction of expression. Whe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ee30fbdada29342edf98d7a908f046ae
https://doi.org/10.1128/mcb.3.4.580

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Mini-D3112 bacteriophage transposable elements for genetic analysis of Pseudomonas aeruginosa

Autor: Aldis Darzins, M J Casadaban

Publikováno v: Journal of Bacteriology. 171:3909-3916

Small bacteriophage D3112 transposable elements deleted for most of the phage-lytic functions while retaining the sites required for transposition and packaging were constructed to facilitate genetic studies in Pseudomonas aeruginosa. These mini-D de

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc3dd5aee3597332c355445e4d8771a3
https://doi.org/10.1128/jb.171.7.3909-3916.1989

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Purification of the Tn3 transposase and analysis of its binding to DNA

Autor: M A, Fennewald, S P, Gerrard, J, Chou, M J, Casadaban, N R, Cozzarelli

Publikováno v: The Journal of biological chemistry. 256(10)

The transposase encoded by the tnpA gene of Tn3 is a protein specifically required for Tn3 transposition. We have purified it to homogeneity from an Escherichia coli strain containing a mutant Tn3 that overproduces transposase. About a 10-fold additi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::6943abbb26c3451199f0fa13477c8588
https://pubmed.ncbi.nlm.nih.gov/6262296

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