Výsledky vyhledávání - "M Iu, Fonshteĭn"

[Construction and characteristics of a cosmid library of genes of the bacterium Cornyebacterium glutamicum ATSS13032]

Autor: N O, Bukanov, O O, Nashchokina, S A, Borinskaia, A V, Lobashev, M Iu, Fonshteĭn, M M, Gusiatiner, V G, Debabov, N K, Iankovskiĭ

Publikováno v: Genetika. 34(3)

A representative genomic library of the Corynebacterium glutamicum ATCC 13032 genes in a cosmid vector Lorist6 was created. The cosmids contain inserts of bacterial DNA obtained by partial digestion with the Sau3A I restrictase. Five hundred and thir

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::6612fcccd8070813127c74bbbc554277
https://pubmed.ncbi.nlm.nih.gov/9589870

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[Cloning and structural-functional analysis in Escherichia coli of genes of glutamate-producing corynebacteria controlling biosynthesis of amino acids of aspartic acid series]

Autor: O Iu, Beskrovnaia, N O, Bukanov, M Iu, Fonshteĭn, M M, Gusiatiner, A L, Okorokov, O O, Nashchokina, N K, Iankovskiĭ

Publikováno v: Genetika. 26(3)

A library of EcoRI DNA fragments from Brevibacterium flavum was constructed using plasmid vector. The genes complementing ThrA2 and ThrB mutations in Escherichia coli were identified in the library. The gene thrA2 of B. flavum codes for mutant enzyme

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::5742af60b680aa0474dfa717a82a3c9a
https://pubmed.ncbi.nlm.nih.gov/2191895

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[Construction and use of phasmid vectors of large capacity]

Autor: N K, Iankovskiĭ, M Iu, Fonshteĭn, S Iu, Lashina, L M, Ermakova, B A, Rebentish

Publikováno v: Molekuliarnaia biologiia. 21(3)

lambda vector phages--pUC19 phasmid hybrids were constructed. The hybrids, phasmids lambda pMYF131 and lambda pSL51, were used as vector molecules for making genomic libraries. Vector phasmids exist as plasmids in vivo. They contain all the genes and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::94ae67c048ddaa7209b2287d5f6dbcf1
https://pubmed.ncbi.nlm.nih.gov/2821378

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[A new vector for cloning of genes and replicons in yeasts]

Autor: M A, Neĭstat, M Iu, Fonshteĭn, S V, Benevolenskiĭ, N K, Iankovskiĭ, I I, Tolstorukov

Publikováno v: Genetika. 22(11)

A novel Escherichia coli-Saccharomyces cerevisiae shuttle vector lambda MAN78 has been constructed. The vector contains phage lambda 47.1 DNA, Sacch. cerevisiae chromosomal segment with TRP1 gene and the yeast ARS1 replicator. This vector may be prop

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::7663891e1da46499d095dce1b85b19a6
https://pubmed.ncbi.nlm.nih.gov/3542710

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[Phasmids. Properties and the use in genetic engineering. I. Construction of the phasmid vector]

Autor: M Iu, Fonshteĭn, E M, Khurges, N K, Iankovskiĭ, V G, Debasov

Publikováno v: Genetika. 22(8)

A phasmid vector molecule designated pMYF11 has been constructed. The vector combines some useful features of plasmid and phage vector molecules. lambda pMYF11 is a hybrid of lambda 47.1 vector and pBR322 plasmid. CI- marker of pMYF11 is replaced wit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::5d7e74620ed28de0f38a799ab8645557
https://pubmed.ncbi.nlm.nih.gov/3021576

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[Cloning of the regulator gene of Erwinia carotovora repressing pectate lyase ptlA gene expression]

Autor: N O, Bukanov, M Iu, Fonshteĭn, P, Dobrovol'ski, N K, Iankovskiĭ, V G, Debabov

Publikováno v: Antibiotiki i meditsinskaia biotekhnologiia = Antibiotics and medical biotechnology. 32(6)

Pectate lyase synthesis in the cells of Erwinia carotovora ELA 49 is induced by polypectate. This suggested that the Erwinia chromosomes carried a regulator gene responsible for negative regulation of the pectate lyase gene expression. In the present

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::fec60dfd09467704a46d3d7b23cc8bee
https://pubmed.ncbi.nlm.nih.gov/3307613

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[Creation of the library of Bordetella pertussis genome clones and detection of clones containing genes for the synthesis of antigenic determinants of the whooping cough microbe]

Autor: N L, Bakh, M Iu, Fonshteĭn, E M, Kuznetsov, Iu V, Borisenko, N V, Tsvetkova

Publikováno v: Zhurnal mikrobiologii, epidemiologii i immunobiologii. (12)

Escherichia coli clones containing hybrid phasmids with the inserts of B. pertussis DNA were obtained with the use of a phasmid vector. The total amount of the clones thus obtained was 97,000, which considerably exceeded the volume of the clone libra

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::028fc83dbb6e352df3ce766765c3bd66
https://pubmed.ncbi.nlm.nih.gov/2435089

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[Phasmids. Properties and the use in genetic engineering. II. Packing in vitro. Lysogeny of Escherichia coli K-12]

Autor: M Iu, Fonshteĭn, N K, Iankovskiĭ

Publikováno v: Genetika. 22(8)

Circular monomeric lambda DNA molecules were used as a substrate for packaging reaction in vitro. For obtaining lambda DNA in circular monomeric form only, Escherichia coli recA plasmid bearing cells were used. This hybrid DNA molecule which we desig

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::183120a2d75b02854008374b6e4d359f
https://pubmed.ncbi.nlm.nih.gov/3021577

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