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pro vyhledávání: '"M H, Montrose"'
Autor:
M. H. Montrose
Publikováno v:
Journal of Tissue Culture Methods. 13:211-215
A quantitative method is described for the measurement of intracellular Na+ and K+ using flame photometry. This technique allows the determination of Na+ and K+ from a single sample derived from submilligram quantities of cellular protein. The method
Autor:
M H, Montrose
Publikováno v:
The Journal of clinical investigation. 108(12)
Secretion of bicarbonate from epithelial cells is considered to be the primary mechanism by which the duodenal mucosa is protected from acid-related injury. Against this view is the finding that patients with cystic fibrosis, who have impaired duoden
Autor:
M H, Montrose
Publikováno v:
Scanning. 20(3)
Publikováno v:
The Journal of biological chemistry. 273(15)
Na+/H+ exchanger isoform 3 (NHE3), an epithelial brush border isoform of the Na+/H+ exchanger gene family, plays an important role in reabsorption of Na+ in the small intestine, the colon, and the kidney. In several cell types, phorbol 12-myristate 1
Autor:
C, Montrose-Rafizadeh, J, Kole, L M, Bartkowski, L H, Lee, D L, Blackmon, S E, Behnken, J D, Gearhart, J A, Cohn, M H, Montrose
Publikováno v:
Journal of cellular physiology. 170(3)
HT29 cells endogenously express the cystic fibrosis transmembrane conductance regulator (CFTR) and have been used previously as a model to examine cellular regulation of CFTR expression and chloride secretory function. Homologous recombination has be
Publikováno v:
Cell motility and the cytoskeleton. 37(2)
Enterotoxigenic strains of B. fragilis associated with childhood diarrhea produce a 20 kD zinc metalloprotease toxin (BFT). BFT is reported to cleave G-actin in vitro and also causes dramatic rounding and rearrangement of the F-actin cytoskeleton in
Publikováno v:
The Journal of biological chemistry. 268(34)
The kinetics and second messenger regulation of three cloned mammalian intestinal Na+/H+ exchangers were studied using fluorometric techniques. These exchangers, NHE1, NHE2, and NHE3, were stably expressed in PS120 fibroblasts, which lack an endogeno
Publikováno v:
The Journal of biological chemistry. 268(16)
A unique Na+/H+ exchanger isoform, NHE-2, was cloned and characterized. NHE-2 is a protein of 809 amino acids with a calculated size of 90,787. It exhibits overall amino acid identity of 50, 44, and 60% with other cloned mammalian Na+/H+ exchangers N
Publikováno v:
The Journal of biological chemistry. 267(2)
Regulation of Na+/H+ exchange by fetal bovine serum was studied in Caco-2 cells, an established cell line derived from a human colon carcinoma. Cells were grown as polarized monolayers on collagen-coated filters and intracellular pH measured fluorome
Publikováno v:
The Journal of biological chemistry. 266(7)
The gene defective in cystic fibrosis has recently been shown to code for a membrane protein designated the "cystic fibrosis transmembrane conductance regulator" (CFTR) protein. While it has been shown that detectable levels of the mRNA for the norma