Zobrazeno 1 - 6
of 6
pro vyhledávání: '"M H, Kalnoski"'
Publikováno v:
BioTechniques. 11(2)
An HPLC approach for purification and sequencing of double-stranded DNA obtained directly from a PCR is described. This simple and reliable procedure has several advantages; the DNA fragment is rapidly eluted (less than 7 minutes), requires no organi
Publikováno v:
Cell. 38:779-789
A unique post-translational modification of tubulin has previously been described in which a tyrosine residue is reversibly added to the C terminus of the alpha-tubulin subunit. We have prepared peptide antibodies that specifically react (shown by co
Publikováno v:
The Journal of Cell Biology
In many vertebrate nonmuscle cells, the microfilament subunit protein, actin, exists as two isoforms, called beta and gamma, whose sequences differ only in their amino-terminal regions. We have prepared a peptide antibody specifically reactive with t
Publikováno v:
Journal of Cellular Biochemistry. 34:113-124
The cytoskeletal protein actin exists in vertebrates as six different isoforms, which are difficult to identify conclusively because of a high degree (greater than 90%) of overall sequence homology. We have used IEF immunoblotting in combination with
Publikováno v:
Analytical biochemistry. 157(1)
A method has been devised for performing Western blot assays on proteins resolved by isoelectric focusing. Electrophoretic transfer of proteins directly from isoelectric focusing (IEF) tube gels to nitrocellulose sheets allowed their immunoassay with
Publikováno v:
Cell motility and the cytoskeleton. 9(4)
In vertebrate skeletal muscle, the proliferating myoblasts synthesize nonmuscle isoforms of actin, and the cells begin to express muscle-specific actin isoforms during their myogenic differentiation. To study the distributions of the actin isoforms i