Zobrazeno 1 - 10
of 10
pro vyhledávání: '"M F, Turchinskiĭ"'
Publikováno v:
Bioorganicheskaia khimiia. 20(1)
Monofunctional platinum compound [Pt(dien)Cl] Cl and high-affinity antibodies against DNA-[Pt(dien)Cl]Cl were suggested for non-radioactive hybridisation analysis of DNA. The simple labelling procedure was based on mixing the reagents followed by the
Publikováno v:
Bioorganicheskaia khimiia. 17(6)
A new reagent, polyphotobiotin (PPhB), for labelling DNA probes has been obtained using low molecule oligoethylenimine (M 600 Da). Photoreactive group of PPhB is 4-azido-salicylic acid. The procedure is simple and quick, the reaction time being about
Publikováno v:
Molekuliarnaia biologiia. 25(2)
DNA modified by trans diaminedichlorplatinum-II (transDDP) has been suggested as an effective probe for non-isotopic hybridization and high-specific anti-DNA-transDDP antibodies with horse radish peroxidase or alkaline phosphotase conjugated antibodi
Publikováno v:
Bioorganicheskaia khimiia. 16(7)
DNA modified by trans-diamminedichloroplatinum (trans-DDP) is suggested as an effective probe for non-isotopic hybridisation. Highly specific anti trans-DDP-DNA antibodies and peroxidase-conjugated antibodies to antirabbit Ig and protein A system hav
Autor:
T I, Vener, M F, Turchinskiĭ, V D, Knorre, A N, Generalova, Iu V, Lukin, S N, Shcherbo, S I, Turkin, V P, Zubov
Publikováno v:
Bioorganicheskaia khimiia. 16(3)
Method of the latex hybridization analysis has been developed: after hybridization of NA-target with biotinylated probe visualization of hybrids was carried out using latex particles, containing fluorescent dye pyronin G and coated with streptavidin.
Publikováno v:
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (11)
N-vinylpyrrolidone-N(2-hydroxy) acrylamide copolymer-modified porous glass was studied as a support for gel permeation chromatography. Influenza, Sendai, fowl plague viruses and rota-viruses were purified by chromatography on the support. As compared
Publikováno v:
Bioorganicheskaia khimiia. 15(10)
A two-step chemical method of introduction of nonradioactive labels in DNA was proposed. At first step DNA is modified by succinic dihydrazide at pH 5.0 and 95 degrees C, or at pH 4.5 and 37 degrees C in presence of sodium bisulfite. Then FITC or bio
Publikováno v:
Molekuliarnaia biologiia. 22(6)
A two-step chemical method has been developed for the introduction of biotin and other low-molecular derivatives into DNA. The method is based on the interaction of aliphatic diamines with cytosine residues of the polynucleotide chain in the presence
Publikováno v:
Molekuliarnaia biologiia. 19(2)
Direct RNA-protein contacts have been studied by means of ultraviolet-induced (254 nm) cross-links inside complexes of NAcPhe-tRNAPhe, Phe-tRNAPhe and deacylated tRNAPhe with poly(U)-charged 30S subunit of Escherichia coli ribosome. In the first two
Publikováno v:
Bioorganicheskaia khimiia. 11(7)
Tritium labelled E. coli total tRNA and tRNAPhe are prepared by action of thermally activated tritium atoms. The preparations, having the molar radioactivity up to 3.6 Ci/mmol, are useful for functional investigations.