Zobrazeno 1 - 10
of 21
pro vyhledávání: '"M E, Winkler"'
Autor:
M. E. Winkler, Hon-Ming Lam
Publikováno v:
Scopus-Elsevier
We report the first molecular genetic analysis of a pyridoxine 5'-phosphate oxidase, the PdxH gene product of Escherichia coli K-12. Chromosomal insertions in and around pdxH were generated with various transposons, and the resulting phenotypes were
Publikováno v:
Journal of Bacteriology. 173:7395-7400
We show that hisT function is required for normal growth of Escherichia coli K-12, since a lack of hisT-mediated pseudouridine tRNA modification causes a uracil requirement that interferes with cell division. We also show that hisT transcription is p
Autor:
A R, Simon, C, Templin, C, Schröder, G, Laaff, R, Tessmann, M E, Winkler, S, Tacke, J, Denner, B, Lapin, M, Chikobava, C, Patience, G, Steinhoff, V Z, Agrba, A, Haverich, U, Martin
Publikováno v:
Annals of transplantation. 8(3)
The discovery that pig endogenous retroviruses are infectious for human cells in vitro lead to vehement discussions about the possible risk of infection after clinical xenotransplantation. Since PERV transmission to non-human primate cells in vitro h
Autor:
U, Martin, M E, Winkler, M, Id, H, Radeke, L, Arseniev, Y, Takeuchi, A R, Simon, C, Patience, A, Haverich, G, Steinhoff
Publikováno v:
Xenotransplantation. 7(2)
The potential risk of viral transmission in the setting of xenotransplantation has gained major attention. Different porcine cell types have been shown to release retroviral particles, which are infectious for human cell lines in vitro. However, ther
Publikováno v:
Biotechnology and bioengineering. 69(1)
A simple, scalable method for purification of plasmid DNA is described. The method includes modification of the classical alkaline-lysis-based plasmid extraction method by extending the solubilization step from less than 30 min to 24 h. The extractio
Autor:
M. E. Winkler
Publikováno v:
Biochemistry and Molecular Biology of Vitamin B6 and PQQ-dependent Proteins ISBN: 9783034895491
Pyridoxal 5’-phosphate (PLP) biosynthesis has been studied extensively inEscherichia coliK-12 by a combination of biochemical and genetic approaches, and theE. colipathway serves as a benchmark for understanding PLP biosynthesis in other organisms.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::420272381187b0a5a4b3303bec0dcfd2
https://doi.org/10.1007/978-3-0348-8397-9_1
https://doi.org/10.1007/978-3-0348-8397-9_1
Autor:
R S, Harris, G, Feng, K J, Ross, R, Sidhu, C, Thulin, S, Longerich, S K, Szigety, P J, Hastings, M E, Winkler, S M, Rosenberg
Publikováno v:
Mutation research. 437(1)
This paper is an invited Response to a recent Commentary [P.L. Foster, Rev. Mut. Res. 436 (1999) 179-184] entitled "Are adaptive mutations due to a decline in mismatch repair? The evidence is lacking". The Commentary argues that no evidence exists su
Autor:
G, Feng, M E, Winkler
Publikováno v:
BioTechniques. 19(6)
The MutS, MutH and MutL proteins mediate methyl-directed-mismatch (MDM) repair in Escherichia coli and Salmonella typhimurium. These proteins have been developed into powerful tools for screening genomes for polymorphisms and detecting and localizing
Autor:
Bich-Yen Nguyen, Edward O. Travis, J. T. Wetzel, J. Peschke, M. E. Winkler, Y. T. Lii, S. M. Filipiak, Robert W. Fiordalice, C. C. Lee
Publikováno v:
MRS Proceedings. 381
As device geometries continue to scale down, a larger portion of the circuit delay is contributed by interconnects, and the majority of this delay is due to capacitive loading. The replacement of plasma-deposited SiO2 as an intermetal dielectric with
Publikováno v:
Transplantation. 56(6)