Zobrazeno 1 - 10
of 10
pro vyhledávání: '"M E, Kamarck"'
Publikováno v:
Proceedings of the National Academy of Sciences. 79:6327-6331
We have screened a large number of isolated human genomic clones that hybridize to a cloned HLA cDNA probe for their ability to direct the synthesis of HLA-A, -B, and -C surface antigens on mouse L cells following DNA-mediated gene transfer. The surf
Publikováno v:
The Journal of Immunology. 136:3085-3091
LFA-3 is expressed on a wide variety of human cell lines, including those which have been used as recipients for gene transfer of human class I gene products, whereas a murine counterpart is either absent or significantly different such that the anti
Publikováno v:
Methods in enzymology. 147
We have described a transfection method for the isolation of surface antigen genes which requires no mRNA or protein purification. Application of this technique results in the recovery of the entire gene in a single step since selection for expressio
Autor:
J M, Nigro, C W, Schweinfest, A, Rajkovic, J, Pavlovic, S, Jamal, R P, Dottin, J T, Hart, M E, Kamarck, P M, Rae, M D, Carty
Publikováno v:
American journal of human genetics. 40(2)
We describe the first isolation of a human creatine kinase M cDNA clone and its mapping of the gene to human chromosome 19. A human creatine kinase M cDNA clone, pJN2CK-M, harboring a 1,160-bp insert, was isolated by colony hybridization with a previ
Autor:
M E, Kamarck, P D, Gottlieb
Publikováno v:
Journal of immunology (Baltimore, Md. : 1950). 119(2)
Autor:
M E, Kamarck
Publikováno v:
Methods in enzymology. 151
Mouse cells containing human surface antigen genes introduced by cell hybridization or DNA transfection can be labeled by indirect immunofluorescence and isolated using the FACS. As illustrated in this chapter, this methodology facilitates genetic st
Autor:
M. A. Nothdurft, J. J. Nedwin, M. E. Kamarck, J. T. Hart, P. M. M. Rae, S. J. Goebel, T. R. Barnett, J. J. Elting
Genomic DNA and mRNA from the adenocarcinoma cell line LoVo were used to generate L-cell transfectants and a bacteriophage lambda gt11 cDNA clone that express epitopes of carcinoembryonic antigen (CEA). Primary and secondary L-cell transfectants expr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e9ad785f7513fa585476dbc27f156913
https://europepmc.org/articles/PMC298853/
https://europepmc.org/articles/PMC298853/
Publikováno v:
Molecular biologymedicine. 1(3)
We describe an approach to the cloning of cell surface proteins that is independent of messenger RNA isolation. Mouse Ltk- cells are cotransformed with the thymidine kinase gene from Herpes Simplex Virus and total human DNA. Transformants expressing
Publikováno v:
Journal of immunology (Baltimore, Md. : 1950). 136(8)
LFA-3 is expressed on a wide variety of human cell lines, including those which have been used as recipients for gene transfer of human class I gene products, whereas a murine counterpart is either absent or significantly different such that the anti
Autor:
M E, Kamarck, F H, Ruddle
Publikováno v:
Progress in clinical and biological research. 97