Zobrazeno 1 - 10
of 93
pro vyhledávání: '"M, Bushfield"'
Autor:
Gwyn W. Gould, A. J. Marker, E. Galloway, S. Palmer, R. N. M. Macsween, Taichi Nakamura, M. Bushfield
Publikováno v:
Biochemical Pharmacology. 44:1037-1043
Hepatocyte growth factor (HGF) is the most potent known mitogen for hepatocytes in primary culture. However, the mechanisms through which HGF induces hepatocyte proliferation have not been defined. Here we have investigated the role of the adenylate
Autor:
M Bushfield, John T. Knowler, Miles D. Houslay, Graeme Milligan, S L Griffiths, Gregory J. Murphy, Peter J. Parker, S Mollner, Nigel J. Pyne
Publikováno v:
Europe PubMed Central
Levels of the G-protein alpha-subunits alpha-Gi-2, alpha-Gi-3 and the 42 kDa, form of alpha-Gs were markedly decreased in hepatocyte membranes from streptozotocin-diabetic animals as compared with normals. In contrast, no detectable changes in alpha-
Publikováno v:
Progress in drug research. Fortschritte der Arzneimittelforschung. Progres des recherches pharmaceutiques. 54
Neuropeptides have been a subject of considerable interest in the pharmaceutical industry over the last 20 years or more. Many drug discovery teams have contributed to our understanding of neuropeptide biology but no significant drugs that act select
Publikováno v:
The Journal of membrane biology. 173(1)
Using the patch-clamp technique, we have characterized membrane currents in single detrusor smooth muscle cells from rat and human urinary bladder. From the voltage- and Ca(2+)-dependence of the current as well as the single channel conductance we co
Publikováno v:
Progress in drug research. Fortschritte der Arzneimittelforschung. Progres des recherches pharmaceutiques. 51
The ability to discover new lead compounds for novel therapeutic targets is a pivotal step in drug discovery programmes. High-throughput screening (HTS) utilises a number of platforms for the rapid screening of novel targets to accelerate this proces
Publikováno v:
Journal of cellular biochemistry.
Challenge of intact hepatocytes with amylin only succeeded in elevating intracellular cyclic AMP levels and activating phosphorylase in the presence of the cAMP phosphodiesterase inhibitor IBMX. Both amylin and CGRP similarly activated adenylate cycl
Both amylin and calcitonin-gene-related neuropeptide (CGRP) activated adenylate cyclase activity in hepatocyte membranes around 5-fold in a dose-dependent fashion, with EC50 values of 120 +/- 14 and 0.3 +/- 0.14 nM respectively. Whereas amylin exhibi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5a4bf6daa341a82e70ebe42b50b4afb
https://europepmc.org/articles/PMC1134344/
https://europepmc.org/articles/PMC1134344/
Recently, the alpha-subunit of the inhibitory guanine-nucleotide-binding protein Gi2 (alpha-Gi2) has been shown to be a substrate for phosphorylation both by protein kinase C and also by other unidentified kinase(s) which are activated as a result of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1612189341a853602f4464b38e6e7d94
https://europepmc.org/articles/PMC1150139/
https://europepmc.org/articles/PMC1150139/
Publikováno v:
Molecular pharmacology. 38(6)
Tissue levels of 3'-AMP were measured in several rat tissues and the sensitivities of the respective adenylyl cyclases were compared with respect to "P" site-mediated inhibition by 3'-AMP2'-deoxy-3'AMP (2'd3'-AMP), and 2',5'-dideoxyadenosine. IC50 va
Publikováno v:
European journal of biochemistry. 192(2)
Treatment of intact, 32Pi-labelled hepatocytes from lean Zucker rats with a range of agents including 12-O-tetradecanoyl-phorbol 13-acetate (TPA), vasopressin, and angiotensin II elicited substantial increases in the phosphorylation of the alpha-subu