Zobrazeno 1 - 10
of 43
pro vyhledávání: '"Lynn C. Thomason"'
Publikováno v:
mBio, Vol 7, Iss 5 (2016)
ABSTRACT Recombineering, in vivo genetic engineering with bacteriophage homologous recombination systems, is a powerful technique for making genetic modifications in bacteria. Two systems widely used in Escherichia coli are the Red system from phage
Externí odkaz:
https://doaj.org/article/dc11ab68298248cdbc9c9bda1bc36825
Publikováno v:
Current protocols. 2(12)
The technology of recombineering, in vivo genetic engineering, was initially developed in Escherichia coli and uses bacteriophage-encoded homologous recombination proteins to efficiently recombine DNA at short homologies (35 to 50 nt). Because the te
Autor:
Lynn C, Thomason, Donald L, Court
Publikováno v:
PHAGE (New Rochelle, N.Y.). 3(3)
The phage λ
Autor:
Lynn C. Thomason, Donald L. Court
Publikováno v:
PHAGE. 3:153-164
Publikováno v:
Molecular Microbiology.
Publikováno v:
Current Protocols. 3
Autor:
Joshua S. Chappie, Christopher J. Hosford, Carl J. Schiltz, Carolyn Court, Lynn C. Thomason, Myfanwy C. Adams, Donald L. Court
Publikováno v:
Mol Microbiol
SummaryThe CI and Cro repressors of bacteriophage λ create a bistable switch between lysogenic and lytic growth. In λ lysogens, CI repressor expressed from thePRMpromoter blocks expression of the lytic promotersPLandPRto allow stable maintenance of
Assembly of intact, replicating plasmids from linear DNA fragments introduced into bacterial cells, i.e. in vivo cloning, is a facile genetic engineering technology that avoids many of the problems associated with standard in vitro cloning. Here we r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::546df2f3666cdda137c4039f25bd82a5
https://doi.org/10.1101/2022.01.13.476211
https://doi.org/10.1101/2022.01.13.476211
Autor:
Carolyn Court, Gillian Murray, Donald L. Court, Kathleen Morrill, Brenda Shafer, Lynn C. Thomason, Thomas D. Schneider
Publikováno v:
Mol Microbiol
Genetic elements in the bacteriophage λ immunity region contribute to stable maintenance and synchronous induction of the integrated E. coli prophage. There is a bistable switch between lysogenic and lytic growth that is orchestrated by the CI and C
Publikováno v:
ACS Synthetic Biology. 8:1877-1889
Dissimilatory metal-reducing bacteria, particularly those from the genus Shewanella, are of importance for bioremediation of metal contaminated sites and sustainable energy production. However, studies on this species have suffered from a lack of eff