Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Lynn, Conley"'
Publikováno v:
Journal of Chromatography A. 1479:81-86
Trisulfides can be a common post-translational modification in many recombinant monoclonal antibodies. These are a source of product heterogeneity that add to the complexity of product characterization and hence, need to be reduced for consistent pro
Autor:
Sanchayita Ghose, Lynn Conley, Alexis Henry, Douglas Cecchini, John Pieracci, Yinying Tao, Edward Koepf
Publikováno v:
Biotechnology and Bioengineering. 114:813-820
Inclusion of a detergent in protein biotherapeutic purification processes is a simple and very robust method for inactivating enveloped viruses. The detergent Triton X-100 has been used for many years and is part of the production process of several
Publikováno v:
Eng Life Sci
With cell culture titers and productivity increasing in the last few years, pressure has been placed on downstream purification to look at alternative strategies to meet the demand of biotech products with high dose requirements. Even when the upstre
Autor:
Rashmi Kshirsagar, Lynn Conley, Yao-Ming Huang, Edward Koepf, Thomas Ryll, Doug Cecchini, Sethu Siva, An Zhang
Publikováno v:
Engineering in Life Sciences. 16:143-151
Acceleration of development timelines to support material production for early-phase clinical trials has been a continuous goal within the life sciences industry, driven largely by the need to advance an ever increasing number of novel therapeutics i
Publikováno v:
Biotechnology Progress
Protein A affinity chromatography is a central part of most commercial monoclonal antibody and Fc-fusion protein purification processes. In the last couple years an increasing number of new Protein A technologies have emerged. One of these new Protei
Publikováno v:
Biotechnology and Bioengineering. 111:1354-1364
Advances in molecular biology and cell culture technology have led to monoclonal antibody titers in excess of 10 g/L. Such an increase can pose concern to traditional antibody purification processes due to limitations in column hardware and binding c
Autor:
Lynn, Conley, Yinying, Tao, Alexis, Henry, Edward, Koepf, Douglas, Cecchini, John, Pieracci, Sanchayita, Ghose
Publikováno v:
Biotechnology and bioengineering. 114(4)
Inclusion of a detergent in protein biotherapeutic purification processes is a simple and very robust method for inactivating enveloped viruses. The detergent Triton X-100 has been used for many years and is part of the production process of several
Autor:
Sanchayita Ghose, Ryan Caple, Jennifer Zhang, Kevin P. Williams, Lynn Conley, Douglas Cecchini
Publikováno v:
Biotechnology progress. 30(6)
Advances in cell culture expression levels in the last two decades have resulted in monoclonal antibody titers of ≥10 g/L to be purified downstream. A high capacity capture step is crucial to prevent purification from being the bottleneck in the ma
Publikováno v:
Biotechnology and bioengineering. 111(7)
Advances in molecular biology and cell culture technology have led to monoclonal antibody titers in excess of 10 g/L. Such an increase can pose concern to traditional antibody purification processes due to limitations in column hardware and binding c