Dimerization deficiency of enigmatic retinitis pigmentosa-linked rhodopsin mutants

Autor: Birgit Ploier, Lydia N. Caro, Takefumi Morizumi, Kalpana Pandey, Jillian N. Pearring, Michael A. Goren, Silvia C. Finnemann, Johannes Graumann, Vadim Y. Arshavsky, Jeremy S. Dittman, Oliver P. Ernst, Anant K. Menon

Publikováno v: Nature Communications, Vol 7, Iss 1, Pp 1-11 (2016)

Retinitis pigmentosa is often caused by mutations that affect the activity or transport of rhodopsin, but some mutations cause disease even though an apparently functional protein is produced. Here the authors show that three such enigmatic mutants r

Externí odkaz: https://doaj.org/article/94c82e6657484b44baee46822dc7c91b

Miniaturized weak affinity chromatography for ligand identification of nanodiscs-embedded G-protein coupled receptors

Autor: Claire Raingeval, Claire Demesmay, Renaud Wagner, Lucie Hartmann, Lydia N. Caro, Vincent Dugas, Sarah Mohamed-Bouteben, Lucile Lecas, Isabelle Krimm

Publikováno v: Analytica Chimica Acta
Analytica Chimica Acta, Elsevier Masson, 2020, 1113, pp.26-35. ⟨10.1016/j.aca.2020.03.062⟩
Analytica Chimica Acta, 2020, 1113, pp.26-35. ⟨10.1016/j.aca.2020.03.062⟩

Biophysical techniques that enable the screening and identification of weak affinity fragments against a target protein are at the heart of Fragment Based Drug Design approaches. In the case of membrane proteins, the crucial criteria for fragment scr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35e5f72c8df6e533962522c677471707
https://hal.archives-ouvertes.fr/hal-03045118/file/Manuscript_auteurs.pdf

G i - and G s -coupled GPCRs show different modes of G-protein binding

Autor: Scott A. Hollingsworth, Lydia N. Caro, Ron O. Dror, Ned Van Eps, Oliver P. Ernst, Naomi R. Latorraca, Christian Altenbach, Wayne L. Hubbell

Publikováno v: Proceedings of the National Academy of Sciences. 115:2383-2388

More than two decades ago, the activation mechanism for the membrane-bound photoreceptor and prototypical G protein-coupled receptor (GPCR) rhodopsin was uncovered. Upon light-induced changes in ligand-receptor interaction, movement of specific trans

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::fb45e11b7b4d5bb8e7d8016159cad167
https://doi.org/10.1073/pnas.1721896115

G

Autor: Ned, Van Eps, Christian, Altenbach, Lydia N, Caro, Naomi R, Latorraca, Scott A, Hollingsworth, Ron O, Dror, Oliver P, Ernst, Wayne L, Hubbell

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America

Significance Activation of G protein-coupled receptors (GPCRs) initiates conformational shifts that trigger interaction with a specific G-protein subtype from a structurally homologous set. A major unsolved problem is the mechanism by which this sele

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::cc9d4b698aa6fafeb86477d392ecb63e
https://pubmed.ncbi.nlm.nih.gov/29463720

Conformational equilibria of light-activated rhodopsin in nanodiscs

Autor: Takefumi Morizumi, Ana Karin Kusnetzow, Lydia N. Caro, Ned Van Eps, Oliver P. Ernst, Timothy H. Bayburt, Klaus Peter Hofmann, Wayne L. Hubbell, Stephen G. Sligar, Michal Szczepek

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America, vol 114, iss 16

Conformational equilibria of G-protein–coupled receptors (GPCRs) are intimately involved in intracellular signaling. Here conformational substates of the GPCR rhodopsin are investigated in micelles of dodecyl maltoside (DDM) and in phospholipid nan

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c25c920a317666de4cdfecbb0df4df56
https://escholarship.org/uc/item/3c01m5gt