Zobrazeno 1 - 10
of 33
pro vyhledávání: '"Lydia Blachowicz"'
Autor:
Ahmed Rohaim, Bram J. A. Vermeulen, Jing Li, Felix Kümmerer, Federico Napoli, Lydia Blachowicz, João Medeiros-Silva, Benoît Roux, Markus Weingarth
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-14 (2022)
Constriction of the selectivity filter is assumed to be a hallmark of C-type inactivation in K+ channels. Using different high-resolution methods, this study shows a distinct C-type inactivation mechanism in a KcsA mutant that emulates Kv-channels.
Externí odkaz:
https://doaj.org/article/a33344fb833146c79e427734c6c04f5c
Publikováno v:
Data in Brief, Vol 32, Iss , Pp 106135- (2020)
Barium is a potent blocker of the KcsA potassium channel. A strategy using x-ray crystallography and molecular dynamics (MD) simulation has been used to understand this phenomenon as described in Rohaim et al. [1]. Wild type KcsA is purified to homog
Externí odkaz:
https://doaj.org/article/a9f7cf448b0543e2bdd1bd11dbfe829b
Publikováno v:
Nature Communications, Vol 7, Iss 1, Pp 1-11 (2016)
Tetrameric cationic channels specificity is determined by the sequence and structural conformation of their selectivity filter. Here, the authors show that a cationic channel from Tsukamurella paurometabola is non-selective due to a Ca2+-binding moti
Externí odkaz:
https://doaj.org/article/54ddb1f1acfa493bbfb0cb50effbba4f
Autor:
Catherine A. Reardon, Elizabeth R. Miller, Lydia Blachowicz, John Lukens, Christoph J. Binder, Joseph L. Witztum, Godfrey S. Getz
Publikováno v:
Journal of Lipid Research, Vol 45, Iss 7, Pp 1347-1354 (2004)
This study tests the hypothesis that autoantibodies to oxidation epitopes on oxidized LDL (OxLDL) promote the clearance of OxLDL from the plasma. Human LDL (hLDL) was injected into immune-competent apolipoprotein E-deficient (apoE−/−) mice and im
Externí odkaz:
https://doaj.org/article/9478b38a3ef74a04a801d02efc49b586
Publikováno v:
Journal of Lipid Research, Vol 39, Iss 7, Pp 1372-1381 (1998)
To examine the association of apolipoprotein (apo) E with nascent hepatic lipoproteins we have prepared stable transfectants of the rat hepatoma cell line McA RH7777 expressing the human apoE3 cDNA. When the nascent lipoproteins secreted from control
Externí odkaz:
https://doaj.org/article/954d7dddb59e4fac9b11928fb5edb477
Autor:
Ahmed Rohaim, Tomasz Slezak, Young Hoon Koh, Lydia Blachowicz, Anthony A. Kossiakoff, Benoît Roux
Publikováno v:
Journal of General Physiology. 154
Engineered antibody fragments (Fabs) have made major impacts on structural biology research, particularly to aid structural determination of membrane proteins. Nonetheless, Fabs generated by traditional monoclonal technology suffer from challenges of
Publikováno v:
J Mol Biol
Barium (Ba2+) is a classic permeant blocker of potassium (K+) channels. The “external lock-in effect” in barium block experiments, whereby the binding of external K+ impedes the forward translocation of the blocker, provides a powerful avenue to
Autor:
João Medeiros-Silva, Ahmed Rohaim, Bram J. A. Vermeulen, Federico Napoli, Jing Li, Felix Kümmerer, Markus Weingarth, Benoît Roux, Lydia Blachowicz
A large class of K+ channels display a time-dependent phenomenon called C-type inactivation whereby prolonged activation by an external stimulus leads to a non-conductive conformation of the selectivity filter. C-type inactivation is of great physiol
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::584d3febcdde4720cb75246281db98d3
https://doi.org/10.1101/2021.09.22.461404
https://doi.org/10.1101/2021.09.22.461404
Autor:
Ahmed Rohaim, Bram J. A. Vermeulen, Jing Li, Felix Kümmerer, Federico Napoli, Lydia Blachowicz, João Medeiros-Silva, Benoît Roux, Markus Weingarth
Publikováno v:
Nature communications. 13(1)
C-type inactivation is of great physiological importance in voltage-activated K+ channels (Kv), but its structural basis remains unresolved. Knowledge about C-type inactivation has been largely deduced from the bacterial K+ channel KcsA, whose select
1H, 15N, and 13C resonance assignments of the intrinsically disordered SH4 and Unique domains of Hck
Publikováno v:
Biomolecular NMR Assignments. 13:71-74
Hemopoietic cell kinase (Hck) is an important signaling enzyme and a potential drug target for HIV infections and Bcr/Abl-chronic myeloid leukemia. The protein shares the same SH4-Unique-SH3-SH2-kinase multi-domain architecture as the other 8 members