Zobrazeno 1 - 10
of 28
pro vyhledávání: '"Luuk Loeff"'
Autor:
Luuk Loeff, Alena Kroupova, Igor Asanović, Franziska Boneberg, Moritz Pfleiderer, Andrè Ferdigg, Fabian Ackle, Javier Martinez, Martin Jinek
RTCB is the catalytic subunit of the metazoan tRNA ligase complex (tRNA-LC) that plays essential roles in tRNA biogenesis and unfolded protein response. The catalytic center of RTCB contains a conserved cysteine that is susceptible to metal ion-induc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ca3b0c21d637b3838e4ce747a29ad133
https://doi.org/10.1101/2023.04.06.535761
https://doi.org/10.1101/2023.04.06.535761
Cas9 is a CRISPR-associated endonuclease capable of RNA-guided, site-specific DNA cleavage1-3. The programmable activity of Cas9 has been widely utilized for genome editing applications4-6. Despite extensive studies, the precise mechanism of target D
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::13f05f616275d95068c6cdde87077925
https://doi.org/10.1101/2021.09.16.460614
https://doi.org/10.1101/2021.09.16.460614
Publikováno v:
Nature. 609(7925)
Cas9 is a CRISPR-associated endonuclease capable of RNA-guided, site-specific DNA cleavage1–3. The programmable activity of Cas9 has been widely utilized for genome editing applications4–6, yet its precise mechanisms of target DNA binding and off
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93aea3f29f3570b6b5dc4d742c57703b
https://pubmed.ncbi.nlm.nih.gov/36002571
https://pubmed.ncbi.nlm.nih.gov/36002571
Publikováno v:
Patterns, 2(5)
Patterns
Patterns
Summary Single-molecule techniques allow the visualization of the molecular dynamics of nucleic acids and proteins with high spatiotemporal resolution. Valuable kinetic information of biomolecules can be obtained when the discrete states within singl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::afa47564f60017af13dee51cbdf2a084
http://resolver.tudelft.nl/uuid:9ceae8c7-ef89-463f-a128-122055fa48e4
http://resolver.tudelft.nl/uuid:9ceae8c7-ef89-463f-a128-122055fa48e4
Publikováno v:
Nature
CRISPR-Cas immunity protects prokaryotes against invading genetic elements1. It uses the highly conserved Cas1-Cas2 complex to establish inheritable memory (spacers)2-5. How Cas1-Cas2 acquires spacers from foreign DNA fragments (prespacers) and integ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a8ee76b4eeaae7a2e1e75dd173c849f
CRISPR-Cas immunity protects prokaryotes against foreign genetic elements. CRISPR-Cas uses the highly conserved Cas1-Cas2 complex to establish inheritable memory (spacers). It remains elusive how Cas1-Cas2 acquires spacers from cellular DNA fragments
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::697ea93a9439ef65f9ab048b834b5d58
https://doi.org/10.1101/608976
https://doi.org/10.1101/608976
Autor:
Chirlmin Joo, Luuk Loeff
Publikováno v:
Biophysics of RNA-Protein Interactions ISBN: 9781493997244
Prokaryotes are constantly threatened by a large array of viruses and other mobile genetic elements. The evolutionary arms’ race between these prokaryotes and their invaders has resulted in a wide arsenal of defence mechanisms that enable the host
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f74188310802161c1805625e44f9e324
https://doi.org/10.1007/978-1-4939-9726-8_9
https://doi.org/10.1007/978-1-4939-9726-8_9
Autor:
Luuk Loeff, Mohamed Fareh, Kyu-Hyeon Yeom, Malwina Szczepaniak, Chirlmin Joo, Anna C. Haagsma
Publikováno v:
Methods, 105
The genome and transcriptome are constantly modified by proteins in the cell. Recent advances in single-molecule techniques allow for high spatial and temporal observations of these interactions between proteins and nucleic acids. However, due to the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c3618cea24261069f183b815fa1e9201
https://doi.org/10.1016/j.ymeth.2016.03.022
https://doi.org/10.1016/j.ymeth.2016.03.022
Publikováno v:
Molecular Cell
Molecular Cell 70 (2018) 3
Molecular Cell, 70(3), 385-394
Molecular Cell 70 (2018) 3
Molecular Cell, 70(3), 385-394
CRISPR-Cas provides RNA-guided adaptive immunity against invading genetic elements. Interference in type I systems relies on the RNA-guided Cascade complex for target DNA recognition and the Cas3 helicase/nuclease protein for target degradation. Even
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e06541f8d0e2a3cb7185696a88db825
Autor:
Dinshaw J. Patel, V. Narry Kim, Mohamed Fareh, Boseon Kim, Chirlmin Joo, Sisi Li, Dhirendra K. Simanshu, Luuk Loeff, Hyeshik Chang, Minju Ha
Publikováno v:
The EMBO Journal, 34 (13), 2015
The EMBO Journal
The EMBO Journal
Terminal uridylyl transferases (TUTs) function as integral regulators of microRNA (miRNA) biogenesis. Using biochemistry, single-molecule, and deep sequencing techniques, we here investigate the mechanism by which human TUT7 (also known as ZCCHC6) re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba94ce4c5ba6e04b710239392bc19d21
http://resolver.tudelft.nl/uuid:9c13bc50-f1f4-4b0b-9912-7b160b3dde5a
http://resolver.tudelft.nl/uuid:9c13bc50-f1f4-4b0b-9912-7b160b3dde5a