Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Lukas Deweid"'
Autor:
Lea Kubitz, Sebastian Bitsch, Xiyan Zhao, Kerstin Schmitt, Lukas Deweid, Amélie Roehrig, Elisa Cappio Barazzone, Oliver Valerius, Harald Kolmar, Julien Béthune
Publikováno v:
Communications Biology, Vol 5, Iss 1, Pp 1-14 (2022)
A small-size engineered enzyme, ultraID, is presented for proximity-dependent biotinylation, that shows efficient labeling in mammalian cell culture, E. coli and S. cerevisiae.
Externí odkaz:
https://doaj.org/article/bda3a7b2012d4b0897455b223b4048a3
Autor:
David Fiebig, Jan P. Bogen, Stefania C. Carrara, Lukas Deweid, Stefan Zielonka, Julius Grzeschik, Björn Hock, Harald Kolmar
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 10 (2022)
Yeast-surface display (YSD) is commonly applied to screen Fab immune or naïve libraries for binders of predefined target molecules. However, reformatting of isolated variants represents a time-intensive bottleneck. Herein, we present a novel approac
Externí odkaz:
https://doaj.org/article/07962c2c56b2491d9d889fe9fed966c1
Autor:
Hendrik Schneider, Lukas Deweid, Thomas Pirzer, Desislava Yanakieva, Simon Englert, Bastian Becker, Dr. Olga Avrutina, Prof. Dr. Harald Kolmar
Publikováno v:
ChemistryOpen, Vol 8, Iss 3, Pp 354-357 (2019)
Abstract Antibody‐drug conjugates (ADCs) are multicomponent biomolecules that have emerged as a powerful tool for targeted tumor therapy. Combining specific binding of an immunoglobulin with toxic properties of a payload, they however often suffer
Externí odkaz:
https://doaj.org/article/76a29a2aef284969bb7d60f746c1ce7c
Autor:
Arturo Macarrón Palacios, Julius Grzeschik, Lukas Deweid, Simon Krah, Stefan Zielonka, Thies Rösner, Matthias Peipp, Thomas Valerius, Harald Kolmar
Publikováno v:
Frontiers in Immunology, Vol 11 (2020)
The B-cell receptor (BCR) is a key player of the adaptive immune system. It is a unique part of immunoglobulin (Ig) molecules expressed on the surface of B cells. In case of many B-cell lymphomas, the tumor cells express a tumor-specific and function
Externí odkaz:
https://doaj.org/article/468b049489af42f28de5409908cf76d0
Autor:
Adem Hadjabdelhafid-Parisien, Sebastian Bitsch, Arturo Macarrón Palacios, Lukas Deweid, Harald Kolmar, Joelle N. Pelletier
Publikováno v:
RSC Advances. 12:33510-33515
We present an efficient approach for tag-free, site-specific conjugation of a fully glycosylated antibody using microbial transglutaminase (mTG). We created variants of trastuzumab where a single surface-exposed residue of the human crystallizable fr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55ac72cddf9497062599eadfdfa35f61
https://doi.org/10.1039/d2ra05630e
https://doi.org/10.1039/d2ra05630e
Autor:
David, Fiebig, Jan P, Bogen, Stefania C, Carrara, Lukas, Deweid, Stefan, Zielonka, Julius, Grzeschik, Björn, Hock, Harald, Kolmar
Publikováno v:
Frontiers in bioengineering and biotechnology. 10
Yeast-surface display (YSD) is commonly applied to screen Fab immune or naïve libraries for binders of predefined target molecules. However, reformatting of isolated variants represents a time-intensive bottleneck. Herein, we present a novel approac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::b12d4f8553931ee2187aa79a0573efd3
https://pubmed.ncbi.nlm.nih.gov/35620472
https://pubmed.ncbi.nlm.nih.gov/35620472
Autor:
Cappio Barazzone E, Kerstin Schmitt, Sebastian Bitsch, Lukas Deweid, Julien Béthune, Oliver Valerius, Zhao X, Harald Kolmar, Roehrig A, Kubitz L
Proximity-dependent biotinylation (PDB) combined with mass spectrometry analysis has established itself as a key technology to study protein-protein interactions in living cells. A widespread approach, BioID, uses an abortive variant of the E. coli B
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::82fc4a484bd841b28cad5cab0d3ec5a7
https://doi.org/10.1101/2021.06.16.448656
https://doi.org/10.1101/2021.06.16.448656
Autor:
Lea, Kubitz, Sebastian, Bitsch, Xiyan, Zhao, Kerstin, Schmitt, Lukas, Deweid, Amélie, Roehrig, Elisa Cappio, Barazzone, Oliver, Valerius, Harald, Kolmar, Julien, Béthune
Publikováno v:
Communications biology. 5(1)
Proximity-dependent biotinylation (PDB) combined with mass spectrometry analysis has established itself as a key technology to study protein-protein interactions in living cells. A widespread approach, BioID, uses an abortive variant of the E. coli B
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::8bd969fc323c9890bccb378a078bf034
https://pubmed.ncbi.nlm.nih.gov/35788163
https://pubmed.ncbi.nlm.nih.gov/35788163
Autor:
Lukas Deweid, Aileen Ebenig, Hans-Lothar Fuchsbauer, Olga Avrutina, Norbert E. Juettner, Harald Kolmar
Publikováno v:
ChemBioChem. 20:2411-2419
Microbial transglutaminase (mTG) has recently emerged as a powerful tool for antibody engineering. In nature, it catalyzes the formation of amide bonds between glutamine side chains and primary amines. Being applied to numerous research fields from m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5abd977bd2c4ec93558b6a61ba967443
https://doi.org/10.1002/cbic.201900101
https://doi.org/10.1002/cbic.201900101
Autor:
Desislava Yanakieva, Simon Englert, Bastian Becker, Thomas Pirzer, Harald Kolmar, Lukas Deweid, Olga Avrutina, Hendrik Schneider
Publikováno v:
ChemistryOpen
Antibody‐drug conjugates (ADCs) are multicomponent biomolecules that have emerged as a powerful tool for targeted tumor therapy. Combining specific binding of an immunoglobulin with toxic properties of a payload, they however often suffer from poor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a2392451f18976a794e7873304bdc098
http://europepmc.org/articles/PMC6437811
http://europepmc.org/articles/PMC6437811