Chimeric Antigen by the Fusion of SARS-CoV-2 Receptor Binding Domain with the Extracellular Domain of Human CD154: A Promising Improved Vaccine Candidate

Autor: Ileanet Ávalos, Thailin Lao, Elsa María Rodríguez, Yasser Zamora, Alianet Rodríguez, Ailyn Ramón, Gilda Lemos, Ania Cabrales, Monica Bequet-Romero, Dionne Casillas, Ivan Andújar, Luis Ariel Espinosa, Luis Javier González, Yanitza Alvarez, Yamila Carpio, Mario Pablo Estrada

Publikováno v: Vaccines, Vol 10, Iss 6, p 897 (2022)

COVID-19 is a respiratory viral disease caused by a new coronavirus called SARS-CoV-2. This disease has spread rapidly worldwide with a high rate of morbidity and mortality. The receptor-binding domain (RBD) of protein spike (S) mediates the attachme

Externí odkaz: https://doaj.org/article/0e5e344f03334568b68b67f412e3b629

In-solution buffer-free digestion allows full-sequence coverage and complete characterization of post-translational modifications of the receptor-binding domain of SARS-CoV-2 in a single ESI–MS spectrum

Autor: Gertrudis Rojas, Yury Valdés-Balbín, Dayana Pérez-Martínez, Vicente Verez-Bencomo, Sum Lai Lozada Chang, Isabel González, Ivan Andújar, Daniel G. Rivera, Enso Onill Torres, Luis Javier González, Gleysin Cabrera, Darielys Santana-Medero, Marika Vitikainen, Julio Palacio, Mark Emalfarb, Marta Ayala, Camila Canaán-Haden, Diamilé Roche, Lourdes Hernández, Vladimir Besada, Saloheimo Markku, Mónica Becquet, Glay Chinea, Eulogio Pimentel, Elías Nelson, Yassel Ramos, Luis Ariel Espinosa, Gerardo Guillén, Kathya Rashida de la Luz Hernández, Tamy Boggiano, Beatriz Pérez-Massón, Miladys Limonta, Alejandro Martín, Ronen Tchelet

Publikováno v: Espinosa, L A, Ramos, Y, Andújar, I, Torres, E O, Cabrera, G, Martín, A, Roche, D, Chinea, G, Becquet, M, González, I, Canaán-Haden, C, Nelson, E, Rojas, G, Pérez-Massón, B, Pérez-Martínez, D, Boggiano, T, Palacio, J, Lozada Chang, S L, Hernández, L, de la Luz Hernández, K R, Markku, S, Vitikainen, M, Valdés-Balbín, Y, Santana-Medero, D, Rivera, D G, Vérez-Bencomo, V, Emalfarb, M, Tchelet, R, Guillén, G, Limonta, M, Pimentel, E, Ayala, M, Besada, V & González, L J 2021, ' In-solution buffer-free digestion allows full-sequence coverage and complete characterization of post-translational modifications of the receptor-binding domain of SARS-CoV-2 in a single ESI–MS spectrum ', Analytical and Bioanalytical Chemistry, vol. 413, no. 30, pp. 7559-7585 . https://doi.org/10.1007/s00216-021-03721-w
Analytical and Bioanalytical Chemistry

Subunit vaccines based on the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 provide one of the most promising strategies to fight the COVID-19 pandemic. The detailed characterization of the protein primary structure by mass spectro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a152c916511b98ca4c2e2db9258c9d7
https://cris.vtt.fi/en/publications/3a525b22-9b0f-4e75-afdb-cb7866f4d80e

Stability-indicative and conformation-specific enzyme linked immunosorbent assay for analysis of recombinant human gamma interferon

Autor: Héctor, Santana, Yaima, González, Dayami, Dorta, Reinaldo, Blanco, Yener, Viltres, Joaquín, González, Luis Ariel, Espinosa, Alberto, Leyva, Iraldo, Bello

Publikováno v: Journal of Pharmaceutical and Biomedical Analysis. 222:115070

Recombinant human interferon gamma (rhIFN-γ) is a promising molecule for the treatment of several diseases. A pair of conformation-specific monoclonal antibodies (mAbs) against rhIFN-γ was selected from generated hybridoma cell lines to design a se

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1112f8bbecdd6f84d45b849348dca610
https://doi.org/10.1016/j.jpba.2022.115070

Targeting the hydrophilic regions of recombinant proteins by MS via in-solution buffer-free trypsin digestion

Autor: Elias N Rodríguez, Luis Ariel Espinosa, György Marko-Varga, Luis Javier González, Yassel Ramos, Aniel Sanchez, Vladimir Besada, Lázaro Betancourt, Mónica Bequet-Romero

Publikováno v: European Journal of Mass Spectrometry. 26:230-237

A desalting step using reversed phase chromatography is a common practice prior to mass spectrometry analysis of proteolytic digests in spite of the detrimental exclusion of the hydrophilic peptides. The detection of such peptides is also important f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c717ede40394c74342f9016a75b449c
https://doi.org/10.1177/1469066719893492

SARS-CoV-2 RBD-Tetanus Toxoid Conjugate Vaccine Induces a Strong Neutralizing Immunity in Preclinical Studies

Autor: Enrique Noa, Ubel Ramirez, Tammy Boggiano, Luis Javier González, Gretchen Bergado, Vicente Verez-Bencomo, Laura Rodriguez, Yury Valdés-Balbín, Raine Garrido, Luis Ariel Espinosa, Yanet Climent, Rocmira Perez, Eduardo Ojito, Kalet León Monzón, Jean-Pierre Soubal, Sonsire Fernández, Daniel G. Rivera, Darielys Santana-Mederos, Felix Cardoso, Claudia Acosta, Lauren Quintero, Yanelys Cabrera Infante, Fabrizio Chiodo, Anamary Suarez, Sum Lai Losada, Tays Hernández, Reinaldo Oliva, Franciscary Pi, Manuel G. Ricardo, Belinda Sánchez Ramírez, Dagmar García-Rivera, Annet Valdes, Mario Landys, Cheng Fang, Yanira Méndez, Juliet Enriquez, Yassel Ramos, Humberto Gonzalez, Guang-Wu Chen, Ernesto Relova-Hernández, Tania Carmenate, Françoise Paquet, Gertrudis Rojas, Mildrey Farinas

Publikováno v: ACS Chemical Biology
ACS Chemical Biology, American Chemical Society, 2021, 16 (7), pp.1223-1233. ⟨10.1021/acschembio.1c00272⟩

Controlling the global COVID-19 pandemic depends, among other measures, on developing preventive vaccines at an unprecedented pace. Vaccines approved for use and those in development intend to use neutralizing antibodies to block viral sites binding

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47ed20c7d44f94f684c5d3d1cfeb572b
https://pubmed.ncbi.nlm.nih.gov/34219448