Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Luis A, Ugozzoli"'
Publikováno v:
Analytical Biochemistry. 344:33-42
Quantitative reverse transcription polymerase chain reaction (qRT-PCR) conducted in real time is a powerful tool for measuring messenger RNA (mRNA) levels in biological samples. Multiplex PCR is defined as the simultaneous amplification of two or mor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c7bb1560a4fb3f8f2f77964c3f5d49a
https://doi.org/10.1016/j.ab.2005.06.026
https://doi.org/10.1016/j.ab.2005.06.026
Autor:
Anthony A, Killeen, John W, Breneman, Arlene R, Carillo, Luis A, Ugozzoli, Jimmie D, Lowery, Jason, Liu, Craig S, Hixson
Publikováno v:
Clinical Chemistry. 49:1050-1057
Background: Two mutations in HFE, G845A (amino acid substitution C282Y) and C187G (H63D), are associated with hereditary hemochromatosis. We developed and validated a novel method, linked linear amplification (LLA), for detection of these two mutatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b5be3be15d684c17b491f75bb5be881
https://doi.org/10.1373/49.7.1050
https://doi.org/10.1373/49.7.1050
Autor:
John W. Breneman, R. Bruce Wallace, Jimmie D. Lowery, Richard D. Press, Antonio A. Reyes, C S Hixson, Luis A. Ugozzoli
Publikováno v:
Clinical Chemistry. 47:31-40
Background: Linked Linear Amplification (LLA) is a new nucleic acid amplification method that uses multiple cycles of primer extension reactions. The presence of nonreplicable elements in LLA primers renders primer extension products unusable as temp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::70e42c670020eeb22bb4bb74c6d3c858
https://doi.org/10.1093/clinchem/47.1.31
https://doi.org/10.1093/clinchem/47.1.31
Autor:
Renzo Galanello, Antonino Giambona, A. Loutradi, Aurelio Maggio, L. Macioni, F. Locati, Jimmie D. Lowery, Luis A. Ugozzoli, Ching-I Patsy Lin, M. Boussiou, Antonio A. Reyes, R. Bruce Wallace, A. Re
Publikováno v:
American Journal of Hematology. 59:214-222
We describe the evaluation of the Bio-Rad BeTha Gene 1 kit (Bio-Rad Laboratories, Hercules, CA), a DNA-probe assay designed for the qualitative determination of the eight most common Mediteranean β-thalassemia mutations. The kit utilizes the princip
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::cbbf923870d941b7b7667c359a0b2b69
https://doi.org/10.1002/(sici)1096-8652(199811)59:3<214::aid-ajh6>3.0.co
https://doi.org/10.1002/(sici)1096-8652(199811)59:3<214::aid-ajh6>3.0.co
Autor:
Ching I P Lin, Elena Cardillo, Luis A. Ugozzoli, Matthew Go, R. Bruce Wallace, Paola Carrera, Antonio A. Reyes, Maurizio Ferrari, Jlmmie D. Lowery
Publikováno v:
Clinical Chemistry. 43:40-44
We can detect the β-globin gene sickle cell mutation by using an assay based on the ligase chain reaction. The simultaneous amplification of the human growth hormone gene in the same reaction serves as a control for the amount of template DNA or amp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2531a345a1c66ce4ee3ae79b9bd579f5
https://doi.org/10.1093/clinchem/43.1.40
https://doi.org/10.1093/clinchem/43.1.40
Autor:
Luis A. Ugozzoli
Publikováno v:
Clinical Chemistry. 50:1963-1965
After the completion of the Human Genome Project, the goal for biomedical research is to apply genomic approaches for the improvement of human health (1). The study of human DNA variation promises to have a great impact on understanding how genetic f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b0094b4a524b55e7fbef93c51fb2ff0
https://doi.org/10.1373/clinchem.2004.039784
https://doi.org/10.1373/clinchem.2004.039784
Publikováno v:
Journal of Visualized Experiments.
The use of siRNA mediated gene knockdown is continuing to be an important tool in studies of gene expression. siRNA studies are being conducted not only to study the effects of downregulating single genes, but also to interrogate signaling pathways a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::435c0bd7a93b156d4c9dee61b3fe48ff
https://doi.org/10.3791/1904-v
https://doi.org/10.3791/1904-v
Publikováno v:
Journal of Visualized Experiments : JoVE
It is becoming increasingly apparent that electroporation is the most effective way to introduce plasmid DNA or siRNA into primary cells. The Gene Pulser MXcell electroporation system and Gene Pulser electroporation buffer (Bio-Rad) were specifically
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f97beddcf06235b4fb231927c643160
https://doi.org/10.3791/1662-v
https://doi.org/10.3791/1662-v
Publikováno v:
Journal of Visualized Experiments : JoVE
It is becoming increasingly apparent that electroporation is the most effective way to introduce plasmid DNA or siRNA into primary cells. The Gene Pulser MXcell electroporation system and Gene Pulser electroporation buffer were specifically developed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73250e270226c2d529fa1e5fe97f0be6
http://europepmc.org/articles/PMC2763291
http://europepmc.org/articles/PMC2763291
Publikováno v:
Methods. 2:42-48
A rapid, non-radioactive approach to the diagnosis of sickle cell anemia is described based on an allele specific polymerase chain reaction (ASPCR) in which the 3'-terminal nucleotide of one of the primers of the primer set forms a match with one all
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8a682c840b54e2a0e9e068aa74760608
https://doi.org/10.1016/s1046-2023(05)80124-0
https://doi.org/10.1016/s1046-2023(05)80124-0