Zobrazeno 1 - 10
of 40
pro vyhledávání: '"Lucyna Lubkowska"'
Autor:
Manchuta Dangkulwanich, Toyotaka Ishibashi, Shixin Liu, Maria L Kireeva, Lucyna Lubkowska, Mikhail Kashlev, Carlos J Bustamante
Publikováno v:
eLife, Vol 2 (2013)
During transcription elongation, RNA polymerase has been assumed to attain equilibrium between pre- and post-translocated states rapidly relative to the subsequent catalysis. Under this assumption, recent single-molecule studies proposed a branched B
Externí odkaz:
https://doaj.org/article/7fa5950ab09845afa9ad63fb17e24c40
Publikováno v:
J Mol Biol
RNA polymerase can cleave a phosphodiester bond at the 3′ end of a nascent RNA in the presence of pyrophosphate producing NTP. Pyrophosphorolysis has been characterized during elongation steps of transcription where its rate is significantly slower
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8dd65b2d874c66050fc7f14e781d0c79
https://doi.org/10.1016/j.jmb.2019.04.020
https://doi.org/10.1016/j.jmb.2019.04.020
Autor:
Maria L. Kireeva, Joshua Turek-Herman, Mikhail Kashlev, V. V. Chasov, Cyndi Trang, Lucyna Lubkowska, Gayane Matevosyan
Publikováno v:
Nucleic Acids Research
Translocation of RNA polymerase (RNAP) along DNA may be rate-limiting for transcription elongation. The Brownian ratchet model posits that RNAP rapidly translocates back and forth until the post-translocated state is stabilized by NTP binding. An alt
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c7870c51e2ef287e819d813829a3543
https://pubmed.ncbi.nlm.nih.gov/29771376
https://pubmed.ncbi.nlm.nih.gov/29771376
Autor:
Mikhail Kashlev, Gary X. Shaw, Ding Jun Jin, Yun-Xing Wang, Smita Kakar, Lucyna Lubkowska, Xinhua Ji, Yan Ning Zhou, Xianyang Fang
Publikováno v:
Journal of Biological Chemistry. 290:23656-23669
Members of the Swi2/Snf2 (switch/sucrose non-fermentable) family depend on their ATPase activity to mobilize nucleic acid-protein complexes for gene expression. In bacteria, RapA is an RNA polymerase (RNAP)-associated Swi2/Snf2 protein that mediates
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8a18be4ab01c444f65f7796db7d28bdd
https://doi.org/10.1074/jbc.m114.618801
https://doi.org/10.1074/jbc.m114.618801
Autor:
Maria L. Kireeva, Mikhail Kashlev, Omar Herrera-Asmat, Daniel G. Guerra, Lucyna Lubkowska, Carlos Bustamante
Recent publications have shown that active RNA polymerase (RNAP) from Mycobacterium tuberculosis (MtbRNAP) can be produced by expressing all four subunits in a single recombinant Escherichia coli strain [1-3]. By reducing the number of plasmids and c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e17ee4e15133bda2ba54a45545b1f2ba
https://hdl.handle.net/20.500.12866/4737
https://hdl.handle.net/20.500.12866/4737
Autor:
Carolyn Court, Donald L. Court, Lucyna Lubkowska, Ding J. Jin, Mikhail Kashlev, Adam R. Parks
Publikováno v:
Nucleic Acids Research
Transcriptional slippage is a class of error in which ribonucleic acid (RNA) polymerase incorporates nucleotides out of register, with respect to the deoxyribonucleic acid (DNA) template. This phenomenon is involved in gene regulation mechanisms and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b2cde7b24f603f6852eeb34359aa824
https://doi.org/10.1093/nar/gku203
https://doi.org/10.1093/nar/gku203
Publikováno v:
Nucleic Acids Research
Cancerous and aging cells have long been thought to be impacted by transcription errors that cause genetic and epigenetic changes. Until now, a lack of methodology for directly assessing such errors hindered evaluation of their impact to the cells. W
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::637100b968bfa09fa9aca4309afff3e1
https://doi.org/10.1093/nar/gkt698
https://doi.org/10.1093/nar/gkt698
Autor:
Deanna Gotte, Maria L. Kireeva, Lucyna Lubkowska, Jeffrey N. Strathern, Jordan D. Irvin, Francisco Malagon, Brenda Shafer, Mikhail Kashlev, Ding Jun Jin
Publikováno v:
Journal of Biological Chemistry. 288:2689-2699
The fidelity of RNA synthesis depends on both accurate template-mediated nucleotide selection and proper maintenance of register between template and RNA. Loss of register, or transcriptional slippage, is particularly likely on homopolymeric runs in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f8a3dde217f1102f686e3d4cc43196ba
https://doi.org/10.1074/jbc.m112.429506
https://doi.org/10.1074/jbc.m112.429506
Significance How does RNA polymerase (RNAP) accurately and orderly control elongation of RNA in nanosystems dominated by random thermal fluctuations? Our statistical approach challenges this open question by identifying a linkage between thermal fluc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::600776c07965c23f5bd09f36dcc67cb1
https://europepmc.org/articles/PMC5127303/
https://europepmc.org/articles/PMC5127303/
Autor:
Patrick Cramer, Jeffrey N. Strathern, Alan C. M. Cheung, Mikhail Kashlev, Maria L. Kireeva, Celine Walmacq, Thomas Carell, Chengcheng Ye, Lucyna Lubkowska, Deanna Gotte
Publikováno v:
Molecular Cell
Summary UV-induced cyclobutane pyrimidine dimers (CPDs) in the template DNA strand stall transcription elongation by RNA polymerase II (Pol II). If the nucleotide excision repair machinery does not promptly remove the CPDs, stalled Pol II creates a r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a94bdcb243e0129877a3707e39b6d01