Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown

Autor: Lucy I. Crouch, Marcelo V. Liberato, Paulina A. Urbanowicz, Arnaud Baslé, Christopher A. Lamb, Christopher J. Stewart, Katie Cooke, Mary Doona, Stephanie Needham, Richard R. Brady, Janet E. Berrington, Katarina Madunic, Manfred Wuhrer, Peter Chater, Jeffery P. Pearson, Robert Glowacki, Eric C. Martens, Fuming Zhang, Robert J. Linhardt, Daniel I. R. Spencer, David N. Bolam

Publikováno v: Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020)

Epithelial cells that line the gut secrete complex glycoproteins that form a mucus layer to protect the gut wall from enteric pathogens. Here, the authors provide a comprehensive characterisation of endo-acting glycoside hydrolases expressed by mucin

Externí odkaz: https://doaj.org/article/34729e0035e248979eeaf2cddb4c661b

Plant N -glycan breakdown by human gut Bacteroides

Autor: Lucy I. Crouch, Paulina A. Urbanowicz, Arnaud Baslé, Zhi-Peng Cai, Li Liu, Josef Voglmeir, Javier M. Melo Diaz, Samuel T. Benedict, Daniel I. R. Spencer, David N. Bolam

Publikováno v: Proceedings of the National Academy of Sciences. 119

The major nutrients available to the human colonic microbiota are complex glycans derived from the diet. To degrade this highly variable mix of sugar structures, gut microbes have acquired a huge array of different carbohydrate-active enzymes (CAZyme

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b22d1f386ecd6734335a17f7785a34c8
https://doi.org/10.1073/pnas.2208168119

Drug‐dependent inhibition of nucleotide hydrolysis in the heterodimeric ABC multidrug transporter PatAB from Streptococcus pneumoniae

Autor: Charlotte Guffick, Pei‐Yu Hsieh, Anam Ali, Wilma Shi, Julie Howard, Dinesh K. Chinthapalli, Alex C. Kong, Ihsene Salaa, Lucy I. Crouch, Megan R. Ansbro, Shoshanna C. Isaacson, Himansha Singh, Nelson P. Barrera, Asha V. Nair, Carol V. Robinson, Michael J. Deery, Hendrik W. van Veen

Funder: Croucher Foundation; Id: http://dx.doi.org/10.13039/501100001692
The bacterial heterodimeric ATP-binding cassette (ABC) multidrug exporter PatAB has a critical role in conferring antibiotic resistance in multidrug-resistant infections by

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81186872eaab2b4eaf3f31791f082d64
https://www.repository.cam.ac.uk/handle/1810/333932

A novel glycosidase plate-based assay for the quantification of galactosylation and sialylation on human IgG

Autor: Daniel I. R. Spencer, Osmond D. Rebello, Paulina A. Urbanowicz, Richard A. Gardner, David N. Bolam, Lucy I. Crouch, David Falck

Publikováno v: Glycoconjugate Journal
Glycoconjugate Journal, 37(6), 691-702. SPRINGER

Changes in human IgG galactosylation and sialylation have been associated with several inflammatory diseases which are a major burden on the health care system. A large body of work on well-established glycomic and glycopeptidomic assays has repeated

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4b247cb22d18f5a8fe56e6bb530159b
https://pubmed.ncbi.nlm.nih.gov/33064245

Engineered photoproteins that give rise to photosynthetically-incompetent bacteria are effective as photovoltaic materials for biohybrid photoelectrochemical cells

Autor: Michael R. Jones, David J. K. Swainsbury, Vincent M. Friebe, David A. Szabo, Lucy I. Crouch, Juntai Liu, Raoul N. Frese

Publikováno v: Faraday Discussions
Liu, J, Friebe, V M, Swainsbury, D J K, Crouch, L I, Szabó, D, Frese, R N & Jones, M 2017, ' Engineered photoproteins that give rise to photosynthetically-incompetent bacteria are effective as photovoltaic materials for biohybrid photoelectrochemical cells ', Faraday Discussions . https://doi.org/10.1039/c7fd00190h
Faraday Discussions, 207, 307-327. Royal Society of Chemistry
Liu, J, Friebe, V M, Swainsbury, D J K, Crouch, L I, Szabo, D A, Frese, R N & Jones, M R 2018, ' Engineered photoproteins that give rise to photosynthetically-incompetent bacteria are effective as photovoltaic materials for biohybrid photoelectrochemical cells ', Faraday Discussions, vol. 207, pp. 307-327 . https://doi.org/10.1039/c7fd00190h

We address concerns that PufX-deficient RCLH1 complexes from photosynthetically-incompetent bacteria may not be suitable as photovoltaic materials for incorporation into biohybrid photoelectrochemical cells.
Reaction centre/light harvesting prot

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e41db5891f01a410740a8d1fc2351e4f
https://doi.org/10.1039/c7fd00190h