Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins: Case Study of the Mouse 5-HT3 Receptor

Autor: Aline Desmyter, Horst Vogel, Lamia Mebarki, Takashi Tomizaki, Ruud Hovius, Romain Wyss, Sonja Minniberger, Xiao-Dan Li, Christophe Moreau, Lucie Peclinovska, Ghérici Hassaine, Cédric Deluz, Hugues Nury, Luigino Grasso, Henning Stahlberg

Publikováno v: Methods in Molecular Biology
Methods in Molecular Biology, Humana Press/Springer Imprint, 2018, pp.139-168. ⟨10.1007/978-1-4939-7151-0_8⟩
Methods in Molecular Biology, 2018, pp.139-168. ⟨10.1007/978-1-4939-7151-0_8⟩
Methods in Molecular Biology ISBN: 9781493971497
Methods in Molecular Biology-Membrane Protein Structure and Function Characterization
Methods in Molecular Biology, Membrane Protein Structure and Function Characterization

International audience; There is growing interest in the use of mammalian protein expression systems, and in the use of antibody-derived chaperones, for structural studies. Here, we describe protocols ranging from the production of recombinant membra

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b97109ac8dfda1b9855a018b3376074a
https://hal.archives-ouvertes.fr/hal-01802945