Prokaryotic expression and characterization of the heterodimeric construction of ZnT8 and its application for autoantibodies detection in diabetes mellitus

Autor: Natalia I. Faccinetti, Luciano L. Guerra, Adriana V. Sabljic, Silvina S. Bombicino, Bruno D. Rovitto, Ruben F. Iacono, Edgardo Poskus, Aldana Trabucchi, Silvina N. Valdez

Publikováno v: Microbial Cell Factories, Vol 16, Iss 1, Pp 1-15 (2017)

Abstract Background In the present work we described the recombinant production and characterization of heterodimeric construction ZnT8-Arg-Trp325 fused to thioredoxin using a high-performance expression system such as Escherichia coli. In addition,

Externí odkaz: https://doaj.org/article/ea68f49773c742448d3236f15cbe0ee1

Characterization of insulin antibodies by Surface Plasmon Resonance in two clinical cases: brittle diabetes and insulin autoimmune syndrome.

Autor: Aldana Trabucchi, Ruben F Iacono, Luciano L Guerra, Natalia I Faccinetti, Andrea G Krochik, María C Arriazu, Edgardo Poskus, Silvina N Valdez

Publikováno v: PLoS ONE, Vol 8, Iss 12, p e84099 (2013)

In this study, the characterization of insulin (auto)antibodies has been described, mainly in terms of concentration (q), affinity (Ka) and Ig (sub)isotypes by Surface Plasmon Resonance (SPR) in two particular clinical cases of individuals with sever

Externí odkaz: https://doaj.org/article/5293088c885d49cc8dedcd4d6d431c54

Decellularized esophageal tubular scaffold microperforated by quantum molecular resonance technology and seeded with mesenchymal stromal cells for tissue engineering esophageal regeneration.

Autor: Marzaro M; Pediatric Surgery Department AULSS2 Treviso, Treviso, Italy., Pozzato G; Telea Electronic Engineering Vicenza, Vicenz, Italy., Tedesco S; Telea Biotech Vicenza, Vicenz, Italy., Algeri M; Department of Pediatric Onco-Hematology and Cell and Gene Therapy, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy., Pozzato A; Telea Electronic Engineering Vicenza, Vicenz, Italy., Tomao L; Department of Pediatric Onco-Hematology and Cell and Gene Therapy, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy., Montano I; Department of Pediatric Onco-Hematology and Cell and Gene Therapy, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy., Torroni F; Digestive Endoscopy and Surgical Unit, Bambino Gesù Children's Hospital, Rome, Italy., Balassone V; Digestive Endoscopy and Surgical Unit, Bambino Gesù Children's Hospital, Rome, Italy., Contini ACI; Digestive Endoscopy and Surgical Unit, Bambino Gesù Children's Hospital, Rome, Italy., Guerra L; Digestive Endoscopy and Surgical Unit, Bambino Gesù Children's Hospital, Rome, Italy., D'Angelo T; Digestive Endoscopy and Surgical Unit, Bambino Gesù Children's Hospital, Rome, Italy., Federici di Abriola G; Digestive Endoscopy and Surgical Unit, Bambino Gesù Children's Hospital, Rome, Italy., Lupoi L; Cen.Ri.S. Policlinico Gemelli UNICATT Rome, Rome, Italy., Caristo ME; Cen.Ri.S. Policlinico Gemelli UNICATT Rome, Rome, Italy., Boškoski I; Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Digestive Endoscopy Unit, Rome, Italy.; Università Cattolica del Sacro Cuore, Centre For Endoscopic Research Therapeutics and Training (CERTT), Rome, Italy-CERTT Gemelli, Rome, Italy., Costamagna G; Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Digestive Endoscopy Unit, Rome, Italy.; Università Cattolica del Sacro Cuore, Centre For Endoscopic Research Therapeutics and Training (CERTT), Rome, Italy-CERTT Gemelli, Rome, Italy., Francalanci P; Pathology Department Bambino Gesù Children's Hospital, Rome, Italy., Astori G; Advanced Cellular Therapy Laboratory, Haematology Unit, San Bortolo Hospital, Vicenza, Italy., Bozza A; Advanced Cellular Therapy Laboratory, Haematology Unit, San Bortolo Hospital, Vicenza, Italy.; Consorzio Per la Ricerca Sanitaria (CORIS) of the Veneto Region, Padova, Italy., Bagno A; Department of Industrial Engineering, University of Padova, Padova, Italy., Todesco M; Department of Industrial Engineering, University of Padova, Padova, Italy., Trovalusci E; Pediatric Surgery Department AULSS2 Treviso, University of Padova, Padova, Italy., Oglio LD; Digestive Endoscopy and Surgical Unit, Bambino Gesù Children's Hospital, Rome, Italy., Locatelli F; Department of Pediatric Onco-Hematology and Cell and Gene Therapy, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.; Department of Pediatrics, Sapienza University of Rome, Roma, Italy., Caldaro T; Digestive Endoscopy and Surgical Unit, Bambino Gesù Children's Hospital, Rome, Italy.

Publikováno v: Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2022 Oct 04; Vol. 10, pp. 912617. Date of Electronic Publication: 2022 Oct 04 (Print Publication: 2022).

Evaluation of Gastroesophageal Reflux Disease 1 Year after Esophageal Atresia Repair: Paradigms Lost from a Single Snapshot?

Autor: Tambucci R; Digestive Endoscopy and Surgery Unit, Bambino Gesù Children's Hospital-IRCCS, Rome, Italy; Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy. Electronic address: renato.tambucci@gmail.com., Isoldi S; Digestive Endoscopy and Surgery Unit, Bambino Gesù Children's Hospital-IRCCS, Rome, Italy; Women's and Children's Health Department, Pediatric Gastroenterology and Hepatology Unit, Sapienza University of Rome, Rome, Italy., Angelino G; Digestive Endoscopy and Surgery Unit, Bambino Gesù Children's Hospital-IRCCS, Rome, Italy., Torroni F; Digestive Endoscopy and Surgery Unit, Bambino Gesù Children's Hospital-IRCCS, Rome, Italy., Faraci S; Digestive Endoscopy and Surgery Unit, Bambino Gesù Children's Hospital-IRCCS, Rome, Italy., Rea F; Digestive Endoscopy and Surgery Unit, Bambino Gesù Children's Hospital-IRCCS, Rome, Italy., Romeo EF; Digestive Endoscopy and Surgery Unit, Bambino Gesù Children's Hospital-IRCCS, Rome, Italy., Caldaro T; Digestive Endoscopy and Surgery Unit, Bambino Gesù Children's Hospital-IRCCS, Rome, Italy., Guerra L; Digestive Endoscopy and Surgery Unit, Bambino Gesù Children's Hospital-IRCCS, Rome, Italy., Contini ACI; Digestive Endoscopy and Surgery Unit, Bambino Gesù Children's Hospital-IRCCS, Rome, Italy., Malamisura M; Digestive Endoscopy and Surgery Unit, Bambino Gesù Children's Hospital-IRCCS, Rome, Italy., Federici di Abriola G; Digestive Endoscopy and Surgery Unit, Bambino Gesù Children's Hospital-IRCCS, Rome, Italy., Francalanci P; Department of Pathology, Bambino Gesù Children's Hospital-IRCCS, Rome, Italy., Conforti A; Neonatal Surgery Unit, Department of Medical and Surgical Neonatology, Bambino Gesù Children's Hospital-IRCCS, Rome, Italy., Dall'Oglio L; Digestive Endoscopy and Surgery Unit, Bambino Gesù Children's Hospital-IRCCS, Rome, Italy., De Angelis P; Digestive Endoscopy and Surgery Unit, Bambino Gesù Children's Hospital-IRCCS, Rome, Italy.

Publikováno v: The Journal of pediatrics [J Pediatr] 2021 Jan; Vol. 228, pp. 155-163.e1. Date of Electronic Publication: 2020 Sep 09.

Evolution of the medical practices and modes of death on pediatric intensive care units in southern Brazil.

Autor: Kipper DJ; Pediatric Intensive Care Unit, Hospital São Lucas, School of Medicine, Pontifícia Universidade Católica do Rio Grande do Sul, Brazil., Piva JP, Garcia PC, Einloft PR, Bruno F, Lago P, Rocha T, Schein AE, Fontela PS, Gava DH, Guerra L, Chemello K, Bittencourt R, Sudbrack S, Mulinari EF, Morais JF

Publikováno v: Pediatric critical care medicine : a journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies [Pediatr Crit Care Med] 2005 May; Vol. 6 (3), pp. 258-63.